equal volume of cell suspension + equal volume of Trypan Blue). Take this into account when determining cell number. Any dilution listed in table is an additional dilution. Live and dead cells listed below represent the total number of cells in 5 large counting squares. *1 x 105 cells/ml cells were seeded into each well of the 24 well plate(s) on day 0 to begin the growth study. Plot this value at the zero time point on your graph.

equal volume of cell suspension + equal volume of Trypan Blue). Take this into account when determining cell number. Any dilution listed in table is an additional dilution. Live and dead cells listed below represent the total number of cells in 5 large counting squares. *1 x 105 cells/ml cells were seeded into each well of the 24 well plate(s) on day 0 to begin the growth study. Plot this value at the zero time point on your graph.

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter2: Basic Hematology

Section2.7: Preparing And Staining A Blood Smear

Problem 12RQ

See similar textbooks

Similar questions

HindII --- 5' GTC - GAC 3', HaeIII --- 5' CC - GG 3', EcoRI --- 5' G - AATTC 3' and BamI --- 5' CCTAG - G 3' 5' AGAATTCTTACGCCGGACGTACCTAGGTTTAGTCGACTC CGCCGCCCCTAGGGTCATCA 3' 3' TCTTAAGAATGCGGCCTGCATGGATCCAAATCAGCTGAGGCGGCGGGGATCCCAGTAGT 5' Number of pieces of DNA , and blunt end fragment (s), and sticky end fragment(s)
regular set Clo drepps la) The lv is In fusing 125 drups Imin with ar How long will It taire to Infuse 25oml?
Organ A Organ B Figure 2 i) Identify organs A and B in Figure 2 above. ii) Give a reason for your choice of answer in e (i) above. iii) State the name of the tissue that is specialized for translocation. Explain how it is adapted to carry out its function.
1 10 20 30 40 50 60 70 -I---- 5' АTCGGTCТCGGCTACTACАТАAАСGCGCGCATATATCGAТАТСТАGСТАGСТАТCGGTCTAGGCTACTАC 3' TAGCCAGAGCCGATGATGTATTTGCGCGCGTATATAGCTATAGATCGATCGATAGCCAGATCCGATGATG I--------I--- --I--- --I------ --I--- -I---------I Promoter 80 90 100 110 120 130 140 -I---- 5' CAGGTATсGGTCTGATCTAССТAGCTTCTтсттстстстстсссссGCGGGGGCTGTACTATСATGCGTCG 3' GTCCATAGCCAGACTAGATCGATCGAAGAGAAGAGAGAGAGGGGGCGCCCCCGACATGATAGTACGCAGC -I- -I------- -I--- -I---------I RBS 150 160 170 180 190 200 210 -----I--- ---I-- ------I- ---I---------I---- -I---------I 5' тстCGGCTАСТАCGTAAACGCGCGCATATAтCGATATCTAGCТAGСТАТСGGTстCGGCTACTAсGTAAA 3' AGAGCCGATGATGCATTTGCGCGCGTATATAGCTATAGATCGATCGATAGCCAGAGCCGATGATGCATTT 220 230 240 250 --I----- ---I-- ------I--- -I 5' CCCTATTAGCATGGGTCATATTTGTGTCTGCTTGTTGGGT 3' GGGATAATCGTACCCAGTAGAAACACAGACGAAGAACCCA a. What are the nucleotides of the MRNA from gene Z? b. What are the amino acids encoded by gene Z? ( Vate V
(continued) Section Date 23 Which cell type that you observed lacks a nucleus? What functions would this cell be unable to carry out? Red bload cells -uithout a hucleus they are wable to trans mit gene tic inf and praide inf. 24 Two drugs are injected into the same thigh muscle. Drug A is at body temperature (37°C) and has a molecular weight of 150 g/mol. Drug B has been in the refrigerator (5°C) and has a molecular weight of 600 g/mol. Predict which drug will diffuse into the blood more rapidly. Explain your reasoning. Dhug A uill difuse faster bc it has highen temp and ID moteaule ueight 25 Isotonic saline and 5% dextrose in water are solutions considered isotonic to human blood. What effect on red blood cells would you expect if a patient were given these fluids intravenously? A solution of 10% dextrose in water is hypertonic to human blood. What would happen if you were to infuse your patient with this solution? 26 Gatorade and other sports drinks are actually hypotonic solutions.…
12/ cell determination is the expression of the cell's specialized role true or false
Part I – SymptomsCallie was 26 years old when she opened a bakery called “Callie’s Cupcakes” in downtown San Francisco with herf ancé, Jeremy. Despite the competitive market, her business was booming; everyone loved the clever recipes and thetrendy atmosphere. Between running their fast-growing business and planning for their wedding, Callie hadn’t beenable to keep to her usual eight hours of sleep a night. Although she had always lived a very healthy lifestyle, exercisingdaily and eating healthy, she just hadn’t been feeling herself lately. She was tired all the time, had dif culty breathing,felt stressed, coughed up sputum, consistently ran a low-grade fever, and had lost weight as her appetite decreased.None of these symptoms alone had been particularly alarming so she had put of seeing her physician for a few weeks.Questions1. What are Callie’s symptoms? List all that were mentioned.2. Based on the symptoms presented, what are three possible respiratory infectious diseases Callie…
5’- TTGACATGCG ATGTCGTAGG GATATATAAT CCACACAGTC GACAATTGAC ATCCCGGTCA CCTATAATCG TGAACACATA TAAGGAGTCT GACTATGGTA AGTAAATATG GTCATTAGTT ATGTGAACAG CCATTGGAGT CCACACTGAC TGAGATTAAG AGATGCCGAT AAAGTGGATA GCATTTTAAC TTGACAATGG ACGATCGATC GTAATTACCA ATGTGAATCG TAGTTGCGCA TTTCGGACGT -3’ a) Counting from the first nucleotide (A) of the Shine-Dalgarno consensus sequence, show the sequence of the first 20 nucleotides in the mRNA encoded by this piece of DNA (specify 5’ & 3’ ends). b) What is the sequence of protein X (specify the amino and carboxyl termini)? c)A mutation occurs in the original DNA strand for protein X such that the adenosine nucleotide at position +35 is deleted. What is the effect of this mutation? d) A mutation occurs such in the original DNA for protein X strand such that the adenosine nucleotide at position +48 is changed to a thymine. What is the effect of this mutation?
signiling event initiated by plasma membrane receptors can generally be divided into sex steps list them
Name the following cells
Discuss about cytokinesis
name all the cells by letter