The number of cells in a culture is estimated based on turbidity. If the following standards are used for comparison: Number of cells/mL Optical Density (measure of turbidity) 0.025 0.050 000'000'৮ 000 000'000' కరరక How many cells are present in a culture that has an optical density of 0.075? O A. Less than 1,000,000 O B. Between 2,000,000 and 4,000,000 O C. 16,000,000 O D. Between 4,000,000 and 8,000,000 Reset Selection

The number of cells in a culture is estimated based on turbidity. If the following standards are used for comparison: Number of cells/mL Optical Density (measure of turbidity) 0.025 0.050 000'000'৮ 000 000'000' కరరక How many cells are present in a culture that has an optical density of 0.075? O A. Less than 1,000,000 O B. Between 2,000,000 and 4,000,000 O C. 16,000,000 O D. Between 4,000,000 and 8,000,000 Reset Selection

Comprehensive Medical Assisting: Administrative and Clinical Competencies (MindTap Course List)

6th Edition

ISBN:9781305964792

Author:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Publisher:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Chapter30: Assisting With Minor Surgery

Section: Chapter Questions

Problem 2CR

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Look at the table below and answer the following questions: Culture 1/10 1/50 1/100 1/200 dilution OD Reading 1 2.0 0.36 0.18 0.11 OD Reading 2 1.9 0.35 0.18 0.08 OD Reading 3 1.8 0.33 0.16 0.09 1. Which 2 dilution levels of the culture are the best to use to compute for its optical density? 2. What is the average optical density of the culture? (use values from 2 dilution levels)
Time point (min) Absorbance of culture at 660nm Approximate cell concentration Approximate # cells in 1mL extract 0 0.298 1.49 x 108 cells/mL 1.49 x 108 cells 10 0.316 1.58 x 108 cells/mL 1.58 x 108 cells 20 0.374 1.87 x 108 cells/mL 1.87 x 108 cells 30 0.429 2.145 x 108 cells/mL 2.145 x 108 cells 40 0.512 2.56 x 108 cells/mL 2.56 x 108 cells 50 0.544 2.72 x 108 cells/mL 2.72 x 108 cells 60 0.607 3.035 x 108 cells/mL 3.035 x 108 cells a. Using these data, prepare a growth curve of this strain ofEscherichia coli (E. coli).b. Estimate the doubling time for this strain of E. Coli. Clearly showhow you estimated this value from the empirical data presented.
Now prepare a 500 ml media having ¼ strength MS with 5mM BAP, 5.5 mg/l Kn and coconut water 10%. Stocks are MS Macro 10X, micro 20X, FeEDTA 10X, Organic 100X, BAP and Kn 5gm/100ml. If you need anything else then you add accordingly. BAP has a MW of 225.3. Sucrose and Myo-inositol is not mentioned in the question. These you need to add. Consistency of the medium is also mentioned.
the optical density of your cell culture is 0.92. How much of this culture should you add to 500 mL of LB to obtain a starting optical density of 0.1?
AY AY * 00 HI T 00 2 3. ment10: Connect Regis.. M https://Ims.mh.. nemical Content of Media i Saved Help Save & Exit Chec Chemically defined medium Complex medium 2 Enriched medium Fastidious Heterotroph 9 Autotroph Match each of the options above to the items below. Uses inorganic carbon as a carbon source for building its own organic molecules. A type of organism that has complex nutrient requirements and requires a nutritionally dense environment. A media containing extracts from other other organisms. A medium with specifically added nutrients to enhance the growth of fastidious organisms. An organism that uses organic carbon as its carbon source. A medium of which the exact chemical composition is known. MacBook Air DD F7 000 000 F2 F4 F5 F8 %23 2$ 4. 9- } 1 Y | 7.
If 0.1 ml is added to 99.9 ml medium, this results in a dilution of: Select one: а. 10-3 b. 10-4 С. 10-1 d. 10-5 e. 10-2
A urine sample is diluted 1:1,000. If 0.1 ml were put into a Petri dish with melted agar, how many CFU/ml are in the urine if 158 colonies grow?
1mL Stock #1 1mL 2000060 9mL #2 9mL wwwww #3 4. 1mL 0000000 A 9mL wooooo #4 0.1m/L O 1mL 5000000 B 9mL #5 1mL 1mL 9mL wwwwww #6 0.1mL 1mk O. D Using the picture serial dilution scheme and the following information (plate A has 276 colonies, plate B has 298, plate C has 2, and plate D has 30), calculate the average number of colony forming units per mL in the stock tube. Make sure to only use countable plates. Round your answer to the nearest one. Write only the number with any needed commas or decimals. Do not include units.
Nicotiana tabacum cells are cultured to produce a polysaccharide gum. The reactor used is a stirred-tank reactor with an initial volume of 100 L. The maximum specific rate of growth of the culture is 0.18 d-1 and the yield coefficient of substrate in biomass is 0.5 gX/gS. The concentration of the limiting substrate in the medium is 3% (m/v). The reactor is inoculated with 1.5 g/L of cells and operated in batch until the substrate is exhausted, when the medium is fed with a constant flow rate of 8 L/d. The fed batch occurs in a quasi-steady state condition.a) Estimate the time of the batch step and the concentration of cells reached in this phase, considering exponential cell growth.b) The fed batch phase is carried out for a period of 40 days. What is the final concentration of cells in the reactor?c) The bioreactor is available for the process for 275 days a year, with an interval of 24 hours between each cultivation. What is the most advantageous operating mode for the process…
Calculate the amount of phycocyanin in Sample 1 in mg where A620=0.204 and A650=0.061, taking into account the dilution factor as per question 6, and the total volume of extract as per question 4. Note your answer to 2 decimal places Sample 1 Total Extract Volume (ml) is 45 Dilution factor is 100 A280 is 1.07 A620 is 0.221 A650 is 00.97 Specific Absorbance Ratio (SAR) A620/A280 is 0.206
You are tasked to prepare 1000mL of 2 media preparations, however, instead of using a powder agar, you will be preparing the desired concentration from a prepared stock solution. Complete the information in the table and show your computation. Volume of Volume of diluent Desired Stock Solution stock sol'n needed to make Concentration needed 1000mL media 25% 12.5% 5% 1.5%
Serial dilution = previous dilution x dilution 11 mL [2] 3 mL Colony count: 100 0.1 mL sample [1] 9.9 mL 5 mL 0.1 ml. Cokony count Dilution: 10-1 10-3 [3] 150 Dilution factor (DF): [4] [5] [6] Compute for the CFU/mL of the sample. Show your solution and express your final answer to 2 significant figures.