Calculate the molecular weight of protein in sample A. Given the protein migration distance is 26 mm and the migration distance of the dye front (gel length) is 6.5 cm.
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1. Calculate the molecular weight of protein in sample A. Given the protein migration distance is 26 mm and the migration distance of the dye front (gel length) is 6.5 cm.
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- (a) 0.25 Turbidity (AU) 0.20- 0.15- 0.10- 0.05- 0.00+ 0 Wt Mutant 1 Mutant 2 Mutant 3 Mutant 4 2 8 Incubation time (h) 6 10 Define turbidity. • How is turbidity related to protein aggregation? • Where is protein with no aggregation for any condition? • Where is protein with maximal aggregation for any condition? • What area of the plot tells you about the rate of aggregation for any condition? 12 • What area of the plot tells you about total aggregation for any condition? • Slow, medium, and fast rates of aggregation are shown - which samples fall into each category? ● Two amounts of total aggregation are shown - which samples fall into each category? • Name two samples that have the similar rates of aggregation, but different amounts of total aggregation. • Name two samples that have different rates of aggregation, but similar amount of total aggregation. ● Why is protein turbidity a useful experiment to perform for a protein especially if you are interested in protein aggregation?A mixture of five proteins are analyzed by MALDI-TOF. Their molecular weigths are 3 kD, 22 kD, 100 kD, 225 kD and 515 kD. A peak appears in the mass spectrum at 33.3 kD. Which protein most likely gave this peak?The A280 of a protein sample loaded onto a gel was determined to be 0.767 (1.00 cm path length, after subtracting the blank). The total volume of this sample was 428 µL. 19.0 µL of this protein sample was mixed with 19.0 µL of 2X laemalli sample buffer and then 12.0 µL of the entire sample was loaded into the gel and electrophoresed. Calculate the amount of protein that was loaded into the gel (in µg).
- If you have the following proteins? Protein MW, kDa 67 pl 3.5 A В 45 7.3 C 45 5.0 D 14.5 7.3 E 67 9.5 a. Describe the methods you will use to separate protein B and D from the rest of the proteins and obtain each in pure form monitor the success of your isolation workflow? Explain | b. How would the native PAGE profile at pH 8 look like for the pure proteins B and D? How would youThis is an SDS-PAGE gel of the protein insulin. The first lane is the molecular weight standard marker. The second lane NR is the native, non-reduced protein (MW 5.7kDa). The third lane is the protein treated with beta-mercaptoethanol. Please explain what is shown in the NR lane versus the R lane.1) If there were two distantly protein, the best BLOSUM & PAM matrix is: ① BLOSUM 45 and PAM 250 ② BLOSUM 45 and PAM 1 ③ BLOSUM 80 and PAM 250 ④ BLOSUM 80 and PAM 1 Give typing answer with explanation and conclusion
- A packing plant fills bags with cement. The weight X kg of a bag of cement can be modelled by a Normal distribution with mean 50 kg and standard deviation of 2kg. a) If a bag is selected is at random, find the probability that the weight of the bag is greater than 55 kg. b) If three bags are selected at random, find the probability that 2 bags weigh more than 55 kg. c) Compute the probability in part a using RStudio.Leni, Isko and Ping are trying to determine the molecular weight of their crude protein samples using SDS-PAGE. After running their samples, they obtained the following bands in their gel. Ladder Leni Isko Ping 1200 Da A 1000 Da 900 Da C 800 Da 700 Da F 600 Da 500 Da 400 Da 300 Da 200 Da 100 Da H Dye front a) Which protein/s is/are present in Leni's sample but not present in Isko and Ping's sample? b) Which protein/s is/are present in Isko's sample but not present in Leni and Ping's sample? c) Using the regression line, calculate the molecular weight of each protein subunits (A-H). |- |- |- 001 2 3 4. 6. 10A protein sample of unknown concentration was placed in a cuvette with a path length of 1 cm and the sample was found to have an A280 value of 2.92. If the protein of interest has an extinction coefficient of 44240 M-1 cm-1 and the molecular weight of the protein is 47.411 kDa, what is the concentration of protein in mg/mL?
- This SDS-PAGE loading buffer has SDS (a denaturing detergent), β-mercaptoethanol (a reducing agent), Coomassie Blue (a dye), and glycerol. You will need to dilute your protein samples such that the final concentration of the loading buffer is 1x, meaning that you will add 5 parts of the protein sample for every 1 part of 6x buffer. Calculate how much of the 6x loading buffer you will need to add to each sample. Then, add the appropriate amount of 6x SDS-PAGE loading buffer to the samples. Store the samples in the -20°C freezer. What is the final concentration of glycerol in each of the samples?A protein has a molecular mass of 400 kDa when measured by size-exclusion chromatography. When subjected to gel electrophoresis in the presence of sodium dodecyl sulfate (SDS), the protein gives three bands with molecularmasses of 180, 160, and 60 kDa. When electrophoresis is carried out in the presence of SDS and dithiothreitol, three bands are again formed, this time with molecular masses of 160, 90, and 60 kDa. Determine the subunit composition of the protein.Assume you have an SAR of 0.206 for sample 1; 0.271 for sample 2; and 0.606 for sample 3, what does this indicate? A larger increase in the purity of phycocyanin is seen after the use of ammonium sulphate, which removes other proteins A larger increase in the purity of phycocyanin is seen after the use of polyethylenimine, which removes other proteins A larger increase in the purity of phycocyanin is seen after the use of polyethylenimine, which removes nucleotides A larger increase in the purity of phycocyanin is seen after the use of ammonium sulphate, which removes nucleotides