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- Table 4- Determination of the optimum pH of catechol oxidase enzyme Potato extract (mL) Tube # 2d 3d 4d 5d 6d 7d dH₂O (mL) 0 0 0 0 0 0 Catechol (mL) 6 6 6 6 6 6 pH buffer added 4mL pH 2 4mL pH 4 4mL pH 6 4mL pH 7 4mL pH 8 4mL pH 10 1 1 1 1 1 1 Absorbance (0 mins) A: 0.010 Start Time: 3:24 A: 0.008 Start Time: 3:34 A: 0.023 Start Time: 3:36 A: -0.006 Start Time: 3:36 0.018 A: Start Time: 3:36 A: 0.011 Start Time: 3:36 Absorbance (after 10 mins in 40°C water bath) -0.030 A: Take reading at: 3:44 A: 0.154 Take reading at: 3:46 A: 0-105 Take reading at: 3:46 A: 0-132 Take reading at: 3:46 A: 0.074 Take reading at: 3:46 A: 0.018 Take reading at: 3!46 Q15) What is the enzyme's optimum pH(s)? Answer: Q16) Use the difference between 2nd and 1st absorbance to support your conclusion for the optimum pH. Subtract Omin from 10min absorbance -0.04 0.0012 0.082 0.138 0.056 0.007Table 2. Volume of BSA, protein content, and absorbance readings of reference solutions Solution Volume of BSA standard solution (μL) Protein content(μg/mL) Absorbance value At 595 nm 1 0 0 0 2 10 1 0.022 3 30 3 0.065 4 50 5 0.106 5 70 7 0.178 6 100 10 0.299 7 120 12 0.380 Make a graph by plotting the absorbance values versus the BSA protein content (in μg) for theseven reference solutions. When constructing the graph, be…5. Examine the following data obtained by a group of students: Tube Results of Lugol's iodine Test Yellow Results of Benedict's Test Blue 1 Orange precipitate Blue 2 3 4 5 Light yellow Dark Purple Dark yellow Yellow Cloudy blue Blue Answer the following questions and explain your answer. a. Which solution contains starch? 3 b. Which solution is most likely glucose? c. Which solution contains the amino acid proline? d. Which solution contains an amino acid other than proline? e. Which tube probably contains only water? f. From the data provided, can you tell which tube contains fats or oils? g. From the data provided, can you tell which tube contains protein? Results of Ninhydrin Test Violet Clear Clear Yellow Clear
- You are growing yeast cells in your lab. The Yeast Growth Medium contains 56 mM glucose (Mol. Weight 180 g/mol) and 20 mM HEPES (MW 238 g/mol). The yeasts only grow if the medium is at the exact pH=6.8. You have HEPES and glucose powder, water, hydrochloric acid (HCl) and Sodium hydroxide (NaOH), all the necessary equipment to prepare the 100 ml that you need. Calculate and explain how you make the Yeast Growth Medium:1. Provide an example of a liquid that has a pH less than 7, one that is neutral and one that is greater than 7. 2. Yeast incubated in NaCl only or Sugar solution?, at room temperature or on ice?The reults for the macroscopic part: 0.30M glycerin – solution was translucent (could see text behind the test tube) 0.15M NaCl – solution was opaque (could not see text behind the test tube) 0.30M NaCl – solution was opaque (could not see text behind the test tube) 0.15M glucose – solution was translucent (could see text behind the test tube) 0.30M glucose – solution was opaque (could not see text behind the test tube) 0.30M Urea – solution was translucent (could see text behind the test tube) Results for microscopic part: 0.30M glycerin – no cells present 0.15M NaCl – normal sized cells 0.30M NaCl – crenated (shrunken and star-shaped) cells 0.15M glucose – no cells present 0.30M glucose – normal sized cells 0.30M Urea – no cells present Determine the osmolarity (hypoosmotic, isosmotic, or hyperosmotic) and tonicity (hypotonic, isotonic, hypertonic) of the following solutions.In which solutions did the osmolarity NOT match the tonicity? For those solutions, why did the osmolarity…
- Give Explanation Detailed Solution of sub parts..(no need handwritten answer)Table 3. Testing for Starch Results ww ww m www Hypothesis (Starch +/-) Starch Present? Sample Initial Color Final Color Potato (1) Onion (2) Starch (3) Water (4) W Words: 363 age: 2 Page: 2 of 4Match the following terms:___Solute (a) A mixture of two or more substances___Mixture in which the molecules of the sub-___Solution stances are evenly distributed___Solvent (b) The medium in which substances are dissolved (c) The substance dissolved in the solvent (d) Two or more substances that combine in any proportion and are not chemi- cally bound
- Part I. For each of the following four questions: calculate and describe how the requested solution would be made. Please show all of your calculations. In all cases the diluent or solvent will be water. Also, assume the smallest volume you can accurately pipette is 1ul. (CaClh: Molecular weight = 111.0 - NaCl: Molecular weight= 58.44) 1. From a 3M CaCh stock solution, you need to make 600 ml of 9 mM CaClh. Indicate how you would make it. 2. You have a 20ul sample of DNA that you want to run in a gel. You are given 5X track dye; how much track dye do you add to your sample so that the track dye concentration in the sample is 1X? 3. You need to make 6 liters of 20% NaCl solution. Indicate how you wvould make it firom powdered NACI.Another source of phosphorus in water is known as organic phosphorus which commonly finds its way into natural bodies of water from human and animal waste and food residues. If you wish to measure the phosphorus content due to organic matter in the water sample using the vanadomolybdophosphoric acid method, you must first "digest" the sample to convert the organic phosphorus to orthophosphate. O True O FalsePrepare 1L 20X Trace minerals stock in a media bottle: Fill out the last column of this table: Component NaCl MnSO4.H₂O H3BO3 CaSO4.5H2O NazMo04.2H₂O COSO4.7H2O KI CaCl2.2H₂O NiCl2 ZnSO4.7H2O FeSO4(NH4)2. 6H20 1X concentration 0.06 g/L 0.024 g/L 0.5 mg/L 0.5 mg/L 2 mg/L 0.0023 mg/L 0.1 mg/L 0.2 g/L 0.0025 mg/L 12 mg/L 35 mg/L 20X (Provide unit)