A mutant form of polypeptide hormone has the following amino acid composition: Asp, Arg, lle, Met, Phe, Pro, Tyr. ValThe following observations are made:Trypsin yields a dipeptide containing Asp and Arg, and a hexapeptide with all the rest.Cyanogen bromide cleavage yields a dipeptide containing Phe and Pro, and a hexapeptide containing all the others.Chymotrypsin cleaves the hormone into two tetrapeptides with the following composition: (Asp, Arg, Tyr, Val) and (lle, Met Phe, Pro)The dipeptide of composition (Pro, Phe) cannot be cleaved by either chymotrypsin or carboxypeptidase.What is the amino acid sequence of this polypeptide?

The amino acid composition of a mutant peptide is Asp, Arg, Ile, Met, Phe, Pro, Tyr, Val. It is an…

Answered: A mutant form of polypeptide hormone…

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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A mutant form of polypeptide hormone has the following amino acid composition:

Asp, Arg, lle, Met, Phe, Pro, Tyr. Val

The following observations are made:

Trypsin yields a dipeptide containing Asp and Arg, and a hexapeptide with all the rest.
Cyanogen bromide cleavage yields a dipeptide containing Phe and Pro, and a hexapeptide containing all the others.
Chymotrypsin cleaves the hormone into two tetrapeptides with the following composition: (Asp, Arg, Tyr, Val) and (lle, Met Phe, Pro)
The dipeptide of composition (Pro, Phe) cannot be cleaved by either chymotrypsin or carboxypeptidase.

What is the amino acid sequence of this polypeptide?

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