PROBLEM 2: A biochemist dliscovers and purifies a new enzyme, generating a purification tablebelowThe step ofproteinpurificationTotalTotalSpecificTotalThe multi-Totalactivityyicld(%)cnzymefoldprotcin(mg)maltifoldactivityincrease inpurification(units)purityWhole-cell lysate8.4LO500l00420040% ammonium22.2952.649.641803990sulfateIon exchange90.22516.6368378755.7Gel filtration505000to01191.811.9(a) From the information given in the table, calculate the specific activity of the enzyme,total yield (%), multi-fold increase in purity, and total multifold purification after eachpurification procedure.b) Which of the purification procedures used for the enzyme is most effective (Le., givesthe greatest relative increase in purity)?(c) Which step - ion-exchange or gel filtration chromatography - causes the greatest lossof the protein.(d) If you wanted to use only one purification step, which technique wouldyouchoose?

Specific activity of enzymes is defined as the activity of the enzyme per milligram of the total…

Answered: (a) From the information given in the…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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(a) From the information given in the table, calculate the specific activity of the enzyme, total yield (%), multi-fold increase in purity, and total multifold purification after each purification procedure,