2. The promoter (P) is the start site of transcription through the binding of RNA polymerase before actual mRNA production. Mutated promoters (P) cannot bind RNA polymerase, so no mRNA is made. In the table below, make predictions about the expression of ß-galactosidase and permease for different partial diploid (F') genotypes. Do this to mark where there is no E. coli growth on two different types of media - one without lactose, and one with. Use a - enzyme produced, and a + to mark where the enzyme is made. The first genotype has all wild-type alleles, and has b- filled in as an example. Genotype I+P+O+Z+Y+ I+P+OcZ+Y- I-P*O*Z*Y*+ I+P+O+Z-Y/IPO*Z*Y* IP OCZ+Y/IPO+Z-Y* ISP+O+Z+Y/I+P+O+*Z-Y* Lactose absent B-galactosidase + Permease + Lactose present B-galactosidase Permease
2. The promoter (P) is the start site of transcription through the binding of RNA polymerase before actual mRNA production. Mutated promoters (P) cannot bind RNA polymerase, so no mRNA is made. In the table below, make predictions about the expression of ß-galactosidase and permease for different partial diploid (F') genotypes. Do this to mark where there is no E. coli growth on two different types of media - one without lactose, and one with. Use a - enzyme produced, and a + to mark where the enzyme is made. The first genotype has all wild-type alleles, and has b- filled in as an example. Genotype I+P+O+Z+Y+ I+P+OcZ+Y- I-P*O*Z*Y*+ I+P+O+Z-Y/IPO*Z*Y* IP OCZ+Y/IPO+Z-Y* ISP+O+Z+Y/I+P+O+*Z-Y* Lactose absent B-galactosidase + Permease + Lactose present B-galactosidase Permease
Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Chapter16: Regulation Of Gene Expression
Section: Chapter Questions
Problem 2TYK: For the E. coli lac operon, when lactose is present: a. and glucose is absent, cAMP binds and...
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