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- 1. Describe how you would go about making up the following solutions. a) 150ml GTE buffer (resuspension solution) containing, 20mM Tris pH 8, 5000µM EDTA, 1M GlucoseIn your own words, explain the Mechanism of Buffer Action.20. List and explain the fluid pH buffering mechanisms both chemical and organ systems. There are more than one.
- 2. Calculate the pH of a buffer containing 0.1 M sodium acetate and 0.2 M acetic acid (pKa = 4.76).4. Which step consists of purification and buffer exchange?5. A 60-year old woman was admitted to the hospital with a diagnosis of pneumonia. She had beenon a thiazide diuretic for 6 months previously for treatment of heart failure. Her lab results were:K+ 2.1 mMpH 7.64PCO2 32 mm Hg PO2 75 mm Hg HCO3- 33 mMClassify her acid-base disturbance. Hint: graph it out on the pH-HCO3- diagram
- 7. · In the space provided, sketch a titration curve (pH vs H+ ions dissociated) of a phosphate buffer shown below. The pKa of H2PO4 is 7.0. H,PO, НРО, + 9. 8. 7 pH 3 1.0 0.5 H+ dissociated --> b. What is the effective buffering range of this acid? At what pH will you have 25% of the buffer in the form of H2PO4? с.Which of the following buffers has the highest buffering capacity? 0.15 M phosphate buffer at pH 7.2 0.015 M phosphate buffer at pH 7.2 0.01 M phosphate buffer at pH 7.2 0.10 M phosphate buffer at pH 7.2Which of the following is a carbonic acid buffering molecule in a basic solution? Group of answer choices H2CO3 HCO3-
- We usually say that a perfect buffer has its pH equal to its pKa. Give an example of a situation in which it would be advantageous to have a buffer with a pH 0.5 unit higher than its pKa.What is the effect of adding a small amount of a strong acid or strong base to the acidity of a buffer? How would this compare to an unbuffered solution of the same acidity?Which of the following is TRUE if pH is higher than the pka of the buffer? O [weak acid] approximately 0 O [conjugate base] = [weak acid] [conjugate base] > [weak acid] O [conjugate base] < [weak acid]