1. The role of cofactors in the realization of the catalytic activity of enzymes.
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- 3. b. If chymotrypsin is treated with disopropylfluorophosphate, catalysis cannot occur even in the presence of excess substrate concentration. Is this Inhibltion reversible or Irreversible?. Dilsopropylfluorophosphate Hyd1. What is the biological purpose of enzyme regulation, i.e., why is it necessary to regulate enzyme activity?11. (ME.23) Given the pathway below, steps catalyzed by enzymes, are good candidates for regulation and compound negatively regulate enzyme 5. A. All steps with -AG; compound H B. Steps catalyzed by enzymes 2 and 5 only; compound C C. Steps catalyzed by enzymes 4 and 5 only; compound H D. Steps catalyzed by enzymes 2, 4, 5, 6 & 8; compound C E. Steps catalyzed by enzymes 2, 4, 5, & 8; compound H A is a good candidate to AG +0.1 enz7 AG AG AG H AG AG +1.1 -23.3 -0.7 -1.4 -16.7 enz1 enz2 enz3 AG enz4 enz5 -
- 5. a) Why would an enzyme that is effective with one reaction have no effect on another reaction?6. Reciprocal regulation of opposing pathways is necessary to avoid the wasteful synthesis anddegradation of metabolic intermediates. Provide two distinct examples of reciprocal regulation. Bespecific, and be sure to explain the conditions that signal enzyme activation and/or inhibition.1. What are the effects of pH and temperature to catalase? What is the optimum pH and optimum temperature for catalase? 2. Explain why the rate of reaction initially increases with increase in temperature then gradually declines as the temperature is further increased. 3. Is the rate of enzymatic reaction always directly dependent on enzyme concentration? Explain. 4. Explain the effect of substrate concentration on enzyme activity. 5. What is the effect of CuSO, on the enzymatic activity of catalase? 6. Is CuSO4 an activator or inhibitor? If it is an inhibitor, what kind of inhibitor is it?
- All coenzymes are cofactors. However, not all cofactors are coenzymes. Both statements are incorrect O The first statement is incorrect while the second statement is correct The first statement is correct while the second statement is incorrect Both statements are correct40. Which of the following statements about transition state analog inhibitors are true? MARK ALL THAT APPLY. Group of answer choices Provide insight into catalytic mechanisms. Very potent and specific inhibitors. They have difficulty binding to the active site because they resemble the energetically unstable transition state of the substrate. Can be used as immunogens to generate catalytic antibodies.18. Explain why the Krebs cycle can be referred to as the final common pathway of thedegradation of organic compounds.
- 8. In patients with diabetes mellitus type I, the biochemical disorders result from changes in fuel metabolism. One of these signs is acidosis. Explain why such patients have a deviation of blood pH from the norm? For this: b) write the reactions of synthesis and oxidation of these molecules, name the enzymes, coenzymes, reaction localization;2. (Part A) Coenzyme-dependent enzymes can catalyze the general transformations shown below. What would be the best coenzymes to use for the two steps in the scheme, and why? R CO₂H A R SCOA B R R12. Mechanisms of activation of enzymes. Examples of different types.