1.a. Jimmy has tasked Carl with aliquoting 18 ul of distilled water into microcentrifuge tubescontaining restriction enzyme. What size micropipettor should Carl use for the most accuratetransfer?b. List the correct volumes for each of the pipettors in the image below. For the P10 provide thevolume in ul and for P200 provide the volume in ul and ml.P10P200149.2. You are given the restriction enzyme Trll with the recognition sequence 5'...TGA^TCA... 3' tocut DNA.a) Would this cut result in blunt or sticky ends? Explain your answer.b) Restriction Enzyme Pmel contains the recognition sequence 5' ...GTTT^AAAC... 3'. Whichrestriction enzyme would result in more cuts, Trll or Pmel? Explain your answer.3. 1. You are doing a DNA extraction using a Wizard kit as the first step to prove Joe Goldberg isguilty of murdering either his girlfriend, her best friend, her brother or her neighbour. Your tubecontains a pellet and a supernatant after adding cold 70% ethanol and centrifuging.a) What do you discard (pellet or supernatant)? Why?b) There was a large amount of blood found on the blanket in Joe's glass box. How much of theblanket do you need for your DNA extraction? Why?c) What solution would you use to resuspend the pellet of the DNA in preparation for PCR?N3

Those exploring chemical characteristics and analyzing reactions, in general, utilize volumetric…

1. a. Jimmy has tasked Carl with aliquoting 18 ul of distilled water into microcentrifuge tubes containing restriction enzyme. What size micropipettor should Carl use for the most accurate transfer? b. List the correct volumes for each of the pipettors in the image below. For the P10 provide the volume in ul and for P200 provide the volume in ul and ml. P10 P200 1 4 2 9. N3

1. a. Jimmy has tasked Carl with aliquoting 18 ul of distilled water into microcentrifuge tubes containing restriction enzyme. What size micropipettor should Carl use for the most accurate transfer? b. List the correct volumes for each of the pipettors in the image below. For the P10 provide the volume in ul and for P200 provide the volume in ul and ml. P10 P200 1 4 2 9. N3

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter10: Reconstitution Of Powdered Drugs

Section: Chapter Questions

Problem 7.2P

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