Experiment 4A + 4B

You add 1 mL of sample and collect 1 mL fractions from a column with a volume of 20 mL where the beads take up 60% of the column and the protein of interest has a partition coefficient of 0.4. What fraction do you want to keep?

6. ( If the solubility of solute Z is 1.0 g/60 mL in diethyl ether and is 1.0 g/1110 mL in water. a) Determine the partition coefficient for the solute Z in these two solvents. b) If 60 mg of the solute Z were dissolved in 100 mL of water, how much ether would be required to extract 90% of solute Z in a single extraction. c) What percent of the A would be extracted from the aqueous solution in (b) by two 50 mL portions of ether?

An unknown substance "A" was allowed to partition between a mixture of 150 mL water and 80 mL benzene. After titration, you found that 0.75 moles of "A" was dissolved in the water while 0.60 moles dissolved in benzene. Calculate for the partition coefficient of substance "A". What can you infer about the nature of substance "A" based on the value of the partition coefficient?

2) Assume you are decaffeinating your coffee by extracting the caffeine out with dichloromethane. Your particular brand of coffee contains 100 mg of caffeine in a 200 mL cup of water. The partition coefficient of caffeine in dichloromethane/water is 8.0 (it is more soluble in the dichloromethane).Answer the following questions about how much caffeine would still be in the 200 mL of water based on two different approaches. You should solve this algebraically. If you use an equation found on the internet, you will need to show how it was derived to receive credit.a) How much caffeine would remain in the water after extracting once with 200 mL of dichloromethane? Show your work.b) How much caffeine would remain in the water after extracting twice, each time with 100 mL of dichloromethane? Show your work.

Simple extractions are particularly useful for separations where only one component has a favorable partition coefficient. True or False?  True  False

Partition coefficients (Kow = organic solvent concentration/aqueous concentration, usually reported as log Kow) are important for determining how efficiently organic compounds can be extracted from aqueous solutions. For example, consider the two substances below. Which will be more soluble in water? Hexamethylbenzene (log Kow = 6.41) O Diethyl ether (log Kow = 0.83)

hi, expert please help me with this problem.. i need it before 5 pm thank you As part of a lab exercise, your group was assigned an unknown compound X for which you had to determine its partition coefficient between an organic solvent and water. You dissolve and shake 4 grams of X in a mixture of 130 mL benzene and 100 mL of water. At the end, your group discovered that the benzene layer contained 0.5 g of X. Use the information provided to calculate for the partition coefficient. What can you say about the chemical nature of compound X?  I HAVE 2 ANSWERS  (i don't know if it is correct hehehe) 1st Answer: partition coefficient (Kd)=conc. at 1st phase (C1) / conc. at 2nd phase (C2) Kd = C1/C2 = CH2O / CBenzene Kd = (4g-0.5g) / 100 mL / 0.5G/130 mL Kd = 0.035/0.003846154 Kd = 9.099 or 9.1   (my 1st answer) My 2nd Answer is on the picture please CHECK IF CORRECT. IF NOT PLEASE SEND THE CORRECT ANSWER THANK YOU.

Ethyl heptanoate (EH), a food aroma compound, was added to water and allowed to come to equilibrium with a known volume of air and results showed that its partition coefficient, [EHair]/[EHwater] is equal to 1.8 x 10-2. In the same experiment with oil in place of water, its partition coefficient, [EHair]/[EHoil] is found to be lower at 6.0 x 10-6. Account for the difference between the two values.

If the concentration in a bulk solution adjacent to a fibrous medium is at a concentration cA = 1mM, and partition coefficient of K = 0.7, what is the concentration in the %3D leading edge of the fibrous medium? the answer has units of mM (canvas does not allow you to enter units or display the answer with units.) your answer mM

4. At 25 °C, 1.0 g of caffeine will dissolve in 47 mL of water, in 8.1 mL of chloroform, in 86 mL of benzene, or in 370 mL of diethyl ether. Calculate the solubility (in grams/100 mL) of caffeine in these four solvents. Calculate the partition coefficient of caffeine between water and each of the other three solvents. Which of these three organic solvents would be the best choice for extracting caffeine from an aqueous solution?

what can we discuss/say on solvent polarity with regards to partition coefficient?

Three extractions with 50 ml portions of chloroform removed 97% of a solute from 200 ml of an aqueous solution. Calculate the partition coefficient of the solute: KD =Cchloro/Caq- Two weak bases (organic amities) have basic dissociation constants of KbA= 1 x 104 and KbB =1 x 10-8. Both bases have partition coefficients of approximately 10 between chloroform and water. Describe a procedure, making use of the difference in K, for separating the two by extraction. Two weak acids, HX and HY, have partition coefficients, Kp = Cet/Caq, of 5 and 50 respectively, and dissociation constants of 1 x 104 and 1 x 108, respectively. Calculate the distribution ratios of the two acids at integral values of pH of the aqueous solution from pH 4 to pH 11, assuming equal volumes of the two solvents. What is the minimum pH for which the ratio of D values is at least 105?

Chemistry A 50ml aliquot of a 0.1 M solution of a neutral drug was extracted with 2 x 5 ml ethyl acetate. The drug concentration in the combined organic layers was found to be 0.36 M. Calculate the partition coefficient for this drug under these conditions.

The partition coefficient for caffeine between dichloromethane and water is 4.6.  Assume that a 100 mL tea solution (in water) contained 100 mg of caffeine.  What is the theoretical yield of caffeine in the methylene chloride if you extract with one 100 mL portion of methylene chloride?

If the partition coefficient of CBN from water into ethanol is 4, what fraction q of the CBN would remain in a 424 mL aqueous sample if it was extracted with 69 mL of ethanol twice?

At 25 °C, the octanol-water equilibrium partition coefficient KOW of Compound X is 150,000 and its water-gas phase equilibrium partition coefficient KWA is 5000. Estimate the octanol-gas phase equilibrium partition coefficient KOA of Compound X.

Acetone has a partition coefficient of 13 while DCM has a partition coefficient of 4.6. Why is DCM used for the extraction of caffeine from Black teabags?

A 36.0 mg sample of an organic compound (molar mass = 84.0 g/mol) is dissolved in 10.0 mL of water. This aqueous solution is extracted with 5.0 mL of hexane. Separation and analysis of the aqueous phase shows that it now contains 12.0 mg of the organic compound. Calculate the partition coefficient for the compound between hexane and water (Kphexane/water). Your answer should reflect the proper number of significant figures.

Variation in volume of extraction solvent: compound X is dissolved in 20.0 mL of solvent B. Assuming a partition coefficient of PX,AB of 5.0, calculate the percentage of X that remains in solvent B if it is extracted the following volumes of solvent A:   1.0 mL 10.0 mL 20.0 mL 100.0 mL

substance of mass 10 g is dissolved in 50 mL of water. Calculate the total mass of sample that can be extracted into 40 mL of ether, if the ether is used in two 20 mL portions .The partition coefficient(k)of the substance between petroleum ether and water is give 4

c) diethyl ether. After separating the layers, 240 mg of cyclohexanol are recovered from the diethyl ether layer. You approximate that the remaining 10 mg of cyclohexanol must have been dissolved in the aqueous layer. Calculate the partition coefficient. Show work. 250 mg of 1-phenylcyclohexanol are dissolved in a combination of 10 mL of water and 20 mL of

Iodine is being extracted from 200 mL water using 50 mL CCl4. The partition coefficient for I2 being extracted from water into CCl4 is 85. i. Calculate the extraction efficiency when the CCl4 is used in one portion. ii. Show how the extraction efficiency is improved if the solvent is used in two portions.

What is the molar extinction coefficient (e) for o-nitrophenol at lmax?  Explain how you arrived at this value.

You have 150 mL of 0.25 M n-propylamine and you shake this in a mixture of 90 mL of water and 50 mL of an organic solvent and let it achieve equilibrium. You removed the water layer and titrated it using 0.30 M HCl, where end point of the titration was achieved at around 42.5 mL. Calculate for the partition coefficient of n-propylamine and describe the affinity of the compound based on the value you obtain.

How would I go about doing this?At 25 °C, the octanol-water equilibrium partition coefficient KOW of Compound X is 150,000 and its water-gas phase equilibrium partition coefficient KWA is 5000. Estimate the octanol-gas phase equilibrium partition coefficient KOA of Compound X.

When a solution of 1.00 g of X in 100 cm³ of water was shaken with 10 cm3 of ether, 0.80 g of X was transferred to the ether layer. Calculate the partition coefficient of X .between ether and water

If the solubility of a drug is 3.8g/L and in chloroform 226g/L calculate its partition coefficient to 1 decimal place