Student Lab Report 4 Notes BIOL 3060 (F23)

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York University *

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3060

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Biology

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Dec 6, 2023

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pdf

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Created by Marishia Agard, TA Coordinator of BIOL 3060 (Fall 2023) 1 Lab 4: Cricket Sensory Nerve Student Notes: In addition to this student lab report notes, please ensure you are still following the lab report marking scheme (that your TA provided for you in the first lab) – you could also watch the video on e-class that goes over how to write a formal lab report for BIOL 3060 Note: This student lab report notes ONLY provide an overview of the following components of the lab report: • Introduction • Methods • Results • Discussion • References Please follow the original lab component scheme, which includes: title page, abstract, introduction, methods, results, discussion, references, and appendix For more information on the other sections of the lab report (title page, abstract, etc.), please see the Student Lab Report Notes for Lab 1 (found on e-class). Lab 4 Overview: Lab 4 examines compound action potentials (CAPs) from sensory spines on the isolated leg from the common cricket ( Acheta domesticus ). These sensory spines are connected to a sensory neuron, which is responsible for detecting external stimuli from the organism's environment. Therefore, any external stimuli that distort the sensory spine will trigger the opening of mechanical-gated ion channels that elicit an action potential to allow electrical impulses to pass through the nerves. In addition, this lab examines how different stimuli , sensory spines , and angles of deflection affect the compound action potentials. Introduction: • Should start the introduction with background information (keywords). Please remember that you should start general and then talk about more specific topics closely related to the lab (upside-down pyramid). Must use references (primary and secondary references) to support background information (Do not use the lab manual as a source!) • Some topics to include in background information: o Sensory hairs/spines in insects (overall importance? – avoid predators) → relate to the nervous system of the animal. o What is a sensory neuron? Describe the components of the sensory neuron. What is the function/role of a sensory neuron in the nervous system? o What is a compound action potential? Describe the stages of an action potential. Nerve impulses and functions of the mechanically gated ion channels.

Created by Marishia Agard, TA Coordinator of BIOL 3060 (Fall 2023) 2 o Introduce the concepts covered in the various exercises [different stimuli examined, the angles of deflection, different spines or sensory hairs respond differently to stimuli]. • Re-introduce rationale and objective. • You are required to include a hypothesis for each exercise (#1-4), and it must be supported by previous literature (primary reference). • Conclude with brief method. Methods: • Please cite the following in the method section to receive the full marks: o ADInstruments. (2010). Cricket Sensory Nerve. • Nothing is else is required for the method section. Results: • You will be responsible for analyzing your data (saved on your USB) collected from the lab. Please refer to the lab manual on how to analyze the data for each exercise. • The results text: You should talk about all results from ALL 4 exercises from the lab. Make sure to highlight trends and patterns you observed. Should be specific and include quantitative data (mean±SEM for key data points). Ensure you refer to the figures/tables in the results text. • The results figures: o Bar graph (exercise 1) → must plot the mean±SEM of the amplitudes measured from 15 spikes for each stimulus ( spontaneous, tap, puff, and tap ) – refer to page 10 of the lab manual. The bar graph would have the following axes: y-axis: Compound action potential amplitude ( 𝜇 V) and x-axis: Stimuli (spontaneous, tap, puff, and touch). Note: The Table on page 10 of the lab manual would be Table 1 in the appendix of the lab report. o Table (exercise 2) → Required to make a table for exercise 2 (page 11 of lab manual). Must include BOTH raw and mean±SEM of the amplitude for 15 spikes each spine . o Bar graph (exercise 3) → must plot the mean±SEM of amplitude for 15 spikes for each angle of deflection – refer to page 12 of the lab manual. The bar graph would have the following axes: y-axis: Compound action potential amplitude ( 𝜇 V) and x-axis: angles of deflection (0 o , 90 o , 180 o , 270 o ). Note: The Table on page 12 of the lab manual would be Table 2 in the appendix of the lab report. • Include figure/table captions (Reminder: Caption title must be bolded, and the caption should be black 12pt Times New Roman font). The figure caption should be detailed by including the following: brief method on how the data was collected, key results, sample size, stats, and mean ± SEM. • No interpretation of the results. • Summary of the results for each exercise:

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