Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 14.5, Problem 1COMQ
For a riboswitch that controls transcription, the binding of a small molecule such as TPP controls whether the RNA
a. has an antiterminator or terminator stem-loop.
b. has a Shine-Dalgarno antisequestor or the Shine-Dalgarno sequence within a stem-loop.
c. is degraded from its 5′ end.
d. has an antiterminator or terminator stem-loop and has a Shine-Dalgarno antisequestor or the Shine-Dalgarno sequence within a stem-loop.
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Which of the following statements is true regarding transcriptional attenuation of the trp operon?
a. The leader peptide encodes a protein that binds to the operator site
b. Translation of the leader peptide stalls when tryptophan levels are high
c. Stalling of the ribosome in region 1 of the leader peptide inhibits formation of the transcriptional terminator
sequence
d. This kind of mechanism can only occur in prokaryotes
e. Both c. and d. are true
Arrange the statements in their proper order by writing the corresponding letter
(e.g. A) for each statement in the space provided below.
A. The single-stranded RNA would complement the target RNA.
B. Gene expression is inactivated once the mRNA is no longer accessible for
translation.
C. The risk-induced silencing complex which is composed of RNA and protein
subunits is formed.
D. Double-stranded, non-coding RNA is cleaved by Dicer.
E. The mRNA can be cleaved or remain bound by the RISC.
1.
2.
3.
4.
5.
Choose the item in column 2 that best matches each item in column 1.
A. Provides information for production of protein
B. Binds to promoter
C. Promoter has a box A and box B consensus sequences
D. Autocatalytic RNA molecules
E. Associated with transcription termination
F. Polymerization of ribonucleotides to form RNA molecules
G. 7-methyl guanosine
H. MRNA prior to processing
select - 1. Core RNA polymerase
select
2. rho (p) factor
select
3. hnRNA
select - 4. RNA
select -5. RNA polymerase holoenzyme
select
6. Ribozymes
select - 7. 5' MRNA cap
select
8. MRNA
Chapter 14 Solutions
Genetics: Analysis and Principles
Ch. 14.1 - 1. A repressor is a __________ that _________...Ch. 14.1 - 2. Which of the following combinations will cause...Ch. 14.2 - 1. What is an operon?
a. A site in the DNA where...Ch. 14.2 - The binding of _______ to lac repressor causes lac...Ch. 14.2 - Prob. 3COMQCh. 14.2 - How does exposing an E. coli cell to glucose...Ch. 14.3 - When tryptophan binds to trp repressor, this...Ch. 14.3 - During attenuation, when tryptophan levels are...Ch. 14.3 - Operons involved with the biosynthesis of...Ch. 14.4 - 1. Translation can be regulated by
a....
Ch. 14.4 - Prob. 2COMQCh. 14.5 - 1. For a riboswitch that controls transcription,...Ch. 14 - 1. What is the difference between a constitutive...Ch. 14 - In general, why is it important to regulate genes?...Ch. 14 - Prob. 3CONQCh. 14 - Transcriptional regulation often involves a...Ch. 14 - An operon is repressible-a small effector molecule...Ch. 14 - Prob. 6CONQCh. 14 - 7. What is enzyme adaptation? From a genetic point...Ch. 14 - Prob. 8CONQCh. 14 - Prob. 9CONQCh. 14 - Prob. 10CONQCh. 14 - Mutations may have an effect on the expression of...Ch. 14 - 12. Would a mutation that inactivated lac...Ch. 14 - Prob. 13CONQCh. 14 - 14. As shown in Figure 14.12, four regions within...Ch. 14 - 15. As described in Chapter 13, enzymes known as...Ch. 14 - 16. The combination of a 3–4 stem-loop and a...Ch. 14 - 17. Mutations in tRNA genes can create tRNAs that...Ch. 14 - Prob. 18CONQCh. 14 - Prob. 19CONQCh. 14 - A species of bacteria can synthesize the amino...Ch. 14 - 21. Using three examples, describe how allosteric...Ch. 14 - How are the actions of lac repressor and trp...Ch. 14 - 23. Transcriptional repressor proteins (e.g., lac...Ch. 14 - Answer the following questions that pertain to the...Ch. 14 - Chapter 21 describes a blotting method known as...Ch. 14 - As described in experimental question E2 and also...Ch. 14 - Prob. 4EQCh. 14 - Explain how the data shown in Figure 14.9 indicate...Ch. 14 - Prob. 6EQCh. 14 - Lets suppose you have isolated a mutant strain of...Ch. 14 - Prob. 1QSDCCh. 14 - Prob. 2QSDC
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- The phosphorylation of the RNA polymerase II tail is important for the following EXCEPT a.the formation of transcription initiation complex b.RNA processing c.the dissociation of most general transcription factors d.initiation of transcription by RNA polymerase IIarrow_forwardTranscription in prokaryotic cells O A. is initiated at a promoter and uses only one strand of DNA (the template strand) to synthesize a complementary RNA strand. B. occurs in the nucleus, whereas translation occurs in the cytoplasm. C. is terminated at a stop codon. D. does not need a promoter. O E. is initiated at a start codon with the help of initiation factors and the small subunit of the ribosome. 5arrow_forwardThere is Hyaluronic acid synthesis occuring in Group X Strep and it is controlled by an operon with 3 genes, called hasXYZ. Based on the 3-line diagram model, a. How many ribosome binding sites are there for the protein? b. How many promoters are there for the genes? c. How many start codons are there for the protein? d. How many RNA Polymerase binding locations are there for the genes? e. How many proteins will be fully functional? f. How many mRNA strands are made?arrow_forward
- David Pribnow studied the base sequences of promoters in bacteria and bacterial viruses. He found two conserved regions in these promoters (the -10 box and the -35 box). These two regions of the promoter Select one: a. signal the initiation site b. bind the sigma subunit that is associated with RNA polymerase O c. attach the correct nucleotide triphosphate to the template DNA strand d. separate the two DNA strandsarrow_forwardAfter treating cells in tissue culture with an HDAC inhibitor, which of the following is the most likely outcome? A. Repression of transcription B. Promoter methylation C. Enhanced splicing D. Activation of transcription E. Depends on the cell typearrow_forwardWhich of the following is not true regarding gene regulation that involves DNA bending? a. The precise distance between the regulatory sequence and the promoter is important. b. Effect can be to repress transcription c. Effect can be to activation transcription d. Regulated genes can be thousands of base pairs away from the regulatory sitesarrow_forward
- Which of the following statements about trp operon regulation is TRUE? A. Low trp levels result in trp repressor binding. O B. High trp levels result in transcriptional antitermination. C. Low trp levels cause the ribosome to fall off at the trp codons in the upstream ORF. O D. High trp levels cause the ribosome to fall off at the trp codons in the upstream ORF. O E. Low trp levels result in transcriptional antitermination. QUESTION 34 Consider the following partial diploid for the lac operon system. lacl+ O+ lac Z+Y-A- lacls O+ 1. 2. lac Z-Y+ A+ In this configuration, which of the following statements is TRUE? A. With no lac present, ZYA will all be expressed. B. with lac present none of the genes (ZYA) will be expressed. O C. With no lac present, only YA will be expressed. D. None of these answers is correct. E. With no lac present, only Z will be expressed.arrow_forwardWhich of the following statements are true about eukaryotic mRNA?a. The sigma factor is essential for the correct initiationof transcription.b. Processing of the nascent mRNA may begin beforeits transcription is complete.c. Processing takes place in the cytoplasm.d. Termination is accomplished by the use of a hairpinloop or the use of the rho factor.e. Many RNAs can be transcribed simultaneously fromone DNA templatearrow_forwardDescribe the following a. Core enzyme RNA polymerase b. Sigma factor c. Promoter consensus sequence d. Rho Dependent Factor e. Intrinsic Factor f. Abortive Initiationarrow_forward
- A principle function of 5' and 3' end modifications of eukaryotic mRNA is: a. to ensure that all nucleotides are phosphorylated b. to protect RNA from nucleolytic degradation c. to guide the removal of introns d. to serve as binding sites for translation release factorsarrow_forwardControl of the trp operon relies on which of the following: a. High levels of tryptophan causing a pause (but not termination) in transcription b. Low levels of tryptophan causing a pause (but not termination) in transcription c. Allolactose binding to the inhibitor to prevent it from binding to the promoter d. Tryptophan binding to the inhibitor to prevent it from binding to the promoterarrow_forwardDefine the following terms: a. chemotaxis b. lac operon c. 80S ribosome d. mRNA secondary structure e. mRNA scanningarrow_forward
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