Concepts of Genetics (12th Edition)
12th Edition
ISBN: 9780134604718
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino, Darrell Killian
Publisher: PEARSON
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Textbook Question
Chapter 11, Problem 1NST
In the Meselson-Stahl experiment, which of the three modes of replication could be ruled out after one round of replication? after two rounds?
Expert Solution
Summary Introduction
To determine: The reason that in the Meselson– Stahl experiment among the three modes of replication, the mode of replication that could be ruled out after oneround of replication.
Introduction: Messelson and Stahl experiment explained that semiconservative mode of replication used by bacterial cells to produce new DNA molecules.
Explanation of Solution
Messelson grew the E. coli cells for many generations in a medium containing 15NH4Cl. These cells then transferred to 14NH4Cl. After one round of replication, the conservative mechanism of DNA replication was ruled out as the intermediate density of DNA band found in the solution. DNA undergoes the semiconservative mode of replication.
Conclusion
Thus, the conservative mode of replication can be ruled out after one round of replication in case of Meselshon Stahl experiment.
Expert Solution
Summary Introduction
To determine: The reason that in Meselson– Stahl experiment among the three modes of replication the mode of replication that could be ruled out after the second round of replication.
Introduction: Messelson and Stahlstudied the modes of DNA replication in bacteria by using nitrogen and its isotope in the medium. Bands of DNA replication was studied after each round of replication.
Explanation of Solution
Messelson grew the bacterial DNA in N15 and N14 media. After one round of replication in14N media, the band of the intermediate density of DNA was found.
After the second round of replication in14N media, the dispersive model of DNA replication was ruled out. The density of the DNA after the second round of replication was between the intermediate (both dark and light band) and “light” densities of DNA.
Conclusion
Thus, the dispersive model of DNA replication was ruled out after the second round of replication.
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Students have asked these similar questions
In the Meselson Stahl experiment differentiating the possible modes of replication, how many rounds of replication were needed to determine that the following modes of replication were not used in E. coli?
two rounds of replication for conservative; two rounds of replication for dispersive
one round of replication for conservative; two rounds of replication for dispersive
two rounds of replication for conservative; one round of replication for dispersive
one round of replication for conservative; one round of replication for dispersive
none of the above is correct
After two generations of replication in the Meselson and Stahl
experiment, what was the composition of the two bands?
One band was all 14N and one band was half 14N and half 15N.
One band was all 15N and one band was half 14N and half 15N.
One band was all 14N and one band was all 15N.
One band was all 15N and one band was one quarter 14N and three
quarters 15N.
One band was all 14N and one band was one quarter 14N and three
quarters 15N.
How many bands of DNA would be expected in Meselson and Stahl’s experiment after two rounds of conservative replication?
Chapter 11 Solutions
Concepts of Genetics (12th Edition)
Ch. 11 - In the Meselson-Stahl experiment, which of the...Ch. 11 - An alien organism was investigated. When DNA...Ch. 11 - Why might mutations in genes encoding telomerase...Ch. 11 - Although the brother is an immunologically matched...Ch. 11 - Prob. 3CSCh. 11 - HOW DOWE KNOW? In this chapter, we focused on how...Ch. 11 - CONCEPT QUESTION Review the Chapter Concepts list...Ch. 11 - Compare conservative, semiconservative, and...Ch. 11 - Describe the role of 15N in the MeselsonStahl...Ch. 11 - Predict the results of the experiment by Taylor,...
Ch. 11 - What are the requirements for in vitro synthesis...Ch. 11 - In Kornbergs initial experiments, it was rumored...Ch. 11 - How did Kornberg assess the fidelity of DNA...Ch. 11 - Which characteristics of DNA polymerase I raised...Ch. 11 - Kornberg showed that nucleotides are added to the...Ch. 11 - What was the significance of the polA1 mutation?Ch. 11 - Summarize and compare the properties of DNA...Ch. 11 - List and describe the function of the ten subunits...Ch. 11 - Distinguish between (a) unidirectional and...Ch. 11 - List the proteins that unwind DNA during in vivo...Ch. 11 - Define and indicate the significance of (a)...Ch. 11 - Outline the current model for DNA synthesis.Ch. 11 - Why is DNA synthesis expected to be more complex...Ch. 11 - Suppose that E. coli synthesizes DNA at a rate of...Ch. 11 - Several temperature-sensitive mutant strains of E....Ch. 11 - While many commonly used antibiotics interfere...Ch. 11 - Describe the end-replication problem in...Ch. 11 - Many of the gene products involved in DNA...Ch. 11 - In 1994, telomerase activity was discovered in...Ch. 11 - The genome of D. melanogaster consists of...Ch. 11 - Prob. 26ESPCh. 11 - DNA polymerases in all organisms add only 5...Ch. 11 - Assume that the sequence of bases shown below is...Ch. 11 - Reiji and Tuneko Okazaki conducted a now classic...Ch. 11 - Consider the drawing of a dinucleotide below. (a)...Ch. 11 - To gauge the fidelity of DNA synthesis, Arthur...
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- In the Meselson-Stahl experiment on DNA replication, what fraction of the DNA was composed of one light strand and one heavy strand ("hybrid") after one generation of growth in medium containing 14N? After two generations of growth in a medium containing 14N? What fraction of hybrid DNA is expected after n generations of growth in a medium containing 14N?arrow_forwardThis is a three part question: Figure B in Box 4.1 illustrates the results of the Meselson-Stahl experiment after a single cycle of replication in 14N. (a) Explain the results they observed after two rounds of replication in 14N medium. (b) Draw out the expected results if a third round of replication were allowed in 14N medium. (c) Two other models for template-directed replication were considered as alternatives to semi-conservative replication. One of these was conservative replication, in which the parental strands were unpaired, replicated, then reannealed such that the parental strands stayed together and the newly synthesized strands were together. The second model was dispersive replication, in which one strand was used as the template for polymerization, then the polymerase switched to using the other strand as the template, and subsequently switched back and forth between the two strands until bother were fully replicated. Each of these models is ruled out by one of your…arrow_forwarda) "Out of three E.coli DNA polymerases, DNA polymerases 3 has a high processivity and rate of polymerization and therefore better suited for replication of the genome" What is meant by processivity? how does the DNA polymerase 3 maintain high processivity? b) What is a replication fork ?. Give the protein/enzymes of a replication fork and describe their function?arrow_forward
- he Meselson and Stahl experiment provided conclusive evidence for the semiconservative replication of DNA in E. coli. What pattern of bands would they have observed in a CsCl gradient after one generation if replication was conservative? a heavy, a light and an intermediate band one heavy and one light band (no intermediate) one intermediate band one light band one heavy bandarrow_forwardWhat are the three models of DNA replication? With the aid of illustrations, show how the Meselson Stahl experiment come to the conclusion of one model of DNA replication. Is DNA replication bidirectional? How did you arrive at this conclusion? Explain the bacterial replication model that supports this conclusion.arrow_forwardA temperature sensitive mutant is isolated where at the non-permissive temperature replication stops relatively slowly and when the DNA is analyzed it appears to have extra twists ahead of the replication forks. What protein is most likely mutated? a)DnaA b)DNA polymerase I c)DNA gyrase d) DNA polymerase IIIarrow_forward
- If the sequence 5′-AACGC-3′ were damaged by reactive oxygen species, what would be the most prevalent product, and what would be the result of replication? (Note: show both strands after replication)arrow_forwardWhat is meant by semi conservative replication? How did Meselson and Stahl prove it experimentally?arrow_forwardIn terms of the new DNA strands that are generated, what are the differences between replication and conventional polymerase chain reaction?arrow_forward
- What observation in the Meslson and Stahl experiment to decipher the DNA replication model distinguishes the semi-conservative from the dispersive mode of replication? Explain.arrow_forwardWhich of the following statements about DNA polymerase I are true? 1) It requires both template DNA and a primer. 2) It can copy and join the ends of single-stranded circular DNA. 3) It uses only DNA as a template primer. 4) With respect to linear DNA, it copies only the 5′ end and then dissociates. a) Only statements 1 and 3 are true. b) Only statements 1 and 4 are true. c) Only statements 1, 2, and 4 are true. d) All of the listed statements are true.arrow_forwardWhich of the following enzymes remove supercoiling in replicating DNA ahead of the replication fork?a) DNA polymerasesb) Helicasesc) Primasesd) Topoisomerasesarrow_forward
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