You obtained the following raw data when setting up a Biuret standard curve:AbsorbancyBSA (mg/ml) 540nm0.15810.2100.2600.3050.3600.4100.45570.5100.5300.550100.554What would the quality of the line-fit (R2 value) be if you do not exclude experimental outliers?(Give you answer to 4 decimal places)O-2N 34 56 o9

The Biuret test is used to find the amount of protein in the given analyte as it checks the presence…

You obtained the following raw data when setting up a Biuret standard curve: BSA (mg/ml) Absorbancy 540nm 0.158 0.210 2 0.260 0.305 0.360 5 0.410 0.455 0.510 8 0.530 9 0.550 10 0.554 What would the quality of the line-fit (R2 value) be if you do not exclude experimental outliers? (Give you answer to 4 decimal places) O1 34 7.

You obtained the following raw data when setting up a Biuret standard curve: BSA (mg/ml) Absorbancy 540nm 0.158 0.210 2 0.260 0.305 0.360 5 0.410 0.455 0.510 8 0.530 9 0.550 10 0.554 What would the quality of the line-fit (R2 value) be if you do not exclude experimental outliers? (Give you answer to 4 decimal places) O1 34 7.

Biomedical Instrumentation Systems

1st Edition

ISBN:9781133478294

Author:Chatterjee

Publisher:Chatterjee

Chapter5: Biomedical Electronics: Digital

Section: Chapter Questions

Problem 5Q

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You record the absorbance of your unknown BSA sample at 0.276. What is the concentration in ug/mL of the unknown assuming you have a standard curve with the line of best fit with y=0.0001x+0.0038 as its equation
The material to be heated is in a long, cylindrical tube, and is viscous enough that any flow inside the tube can be ignored, i.e. the heating is by conduction alone). Also, consider the glass of the tube to be very thin so its thermal resistance can be ignored. The tube diameter is 1 cm and the properties of the material are the same those of water, given by 0.64 W/m*K, 1000 kg/m3, and 4180 J/kg*K, for thermal conductivity, density, and specific heat, respectively. The water bath temperature is 65 °C, and the initial temperature of the material is 27 °C. Without the agitation of the water bath, the heat transfer coefficient can be considered to be 128 W/m2*K. Considering the tube to be a long cylinder, how long does it take for the coldest point to heat up to a temperature corresponding to 99% of the total possible temperature increase? If the surrounding how water is agitated using a stirrer, leading to a considerably large surface heat transfer coefficient, how long does it…
You obtained the following raw data when setting up a Biuret standard curve: Absorbancy BSA (mg/ml) 540nm 0 0.158 1 0.210 2 0.260 3 0.305 4 0.360 5 0.410 6 0.455 7 0.510 8 0.530 9 10 0.550 0.554 After blanking against a biuret-dH20 sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.284 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg/ml (up to 3 significant figures).
You obtained the following raw data when setting up a Bradford standard curve: BSA (mg/ml) Absorbancy 595nm 0 0.225 1 0.310 2 0.420 3 0.510 4 0.610 5 0.720 6 0.810 7 0.915 8 0.950 9 0.980 10 0.990 After blanking against a bradford-dH2O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.570 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).
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