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You have obtained experimental kinetic data for two versions of the same enzyme, a wild-type and a mutant differing from the wild-type at a single amino acid. The data are given in the table. Vmax KM (µmol min-1) (mM) Wild-type 100 10 Mutant 1 0.1 Compare the kinetic parameters of the two versions using the data in the table. Assuming a two-step reaction scheme in which k-j is much larger than k2, which of the following statements are correct? The mutant version has a higher affinity for the substrate. The wild-type version requires a greater concentration of substrate to achieve Vmax - The wild-type version has a higher affinity for the substrate. The mutant version requires a greater concentration of substrate to achieve Vmax -

You have obtained experimental kinetic data for two versions of the same enzyme, a wild-type and a mutant differing from the wild-type at a single amino acid. The data are given in the table. Vmax KM (µmol min-1) (mM) Wild-type 100 10 Mutant 1 0.1 Compare the kinetic parameters of the two versions using the data in the table. Assuming a two-step reaction scheme in which k-j is much larger than k2, which of the following statements are correct? The mutant version has a higher affinity for the substrate. The wild-type version requires a greater concentration of substrate to achieve Vmax - The wild-type version has a higher affinity for the substrate. The mutant version requires a greater concentration of substrate to achieve Vmax -

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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You have obtained experimental kinetic data for two versions of the same enzyme, a wild‑type and a mutant differing from the wild‑type at a single amino acid. The data are given in the table.

 
Compare the kinetic parameters of the two versions using the data in the table.
 
Assuming a two-step reaction scheme in which ?−1 is much larger than ?2, which of the following statements are correct?
 
  • The mutant version has a higher affinity for the substrate.
  • The wild‑type version requires a greater concentration of substrate to achieve ?maxVmax.
  • The wild‑type version has a higher affinity for the substrate.
  • The mutant version requires a greater concentration of substrate to achieve ?maxVmax.

Calculate the initial velocity of the reaction catalyzed by the wild‑type enzyme when the substrate concentration is 10 mM.

The reaction equilibrium is reached once there is no net change in the concentration of the substrate or the product.

Based on the data table and your initial velocity calculation, the reaction equilibrium is altered more in the direction of the product by

  1. wild type version
  2. mutant version
  3. neither version
  4. both versions

 

 

You have obtained experimental kinetic data for two versions of the same enzyme, a wild-type and a mutant differing from the wild-type at a single amino acid. The data are given in the table. Vmax Км (µmol min-1) (mM) Wild-type 100 10 Mutant 1 0.1 Compare the kinetic parameters of the two versions using the data in the table. Assuming a two-step reaction scheme in which k_j is much larger than k2, which of the following statements are correct? The mutant version has a higher affinity for the substrate. The wild-type version requires a greater concentration of substrate to achieve Vmax - The wild-type version has a higher affinity for the substrate. The mutant version requires a greater concentration of substrate to achieve Vmax ·
Transcribed Image Text:You have obtained experimental kinetic data for two versions of the same enzyme, a wild-type and a mutant differing from the wild-type at a single amino acid. The data are given in the table. Vmax Км (µmol min-1) (mM) Wild-type 100 10 Mutant 1 0.1 Compare the kinetic parameters of the two versions using the data in the table. Assuming a two-step reaction scheme in which k_j is much larger than k2, which of the following statements are correct? The mutant version has a higher affinity for the substrate. The wild-type version requires a greater concentration of substrate to achieve Vmax - The wild-type version has a higher affinity for the substrate. The mutant version requires a greater concentration of substrate to achieve Vmax ·
Calculate the initial velocity of the reaction catalyzed by the wild-type enzyme when the substrate concentration is 10 mM. -1 Vo µmol min The reaction equilibrium is reached once there is no net change in the concentration of the substrate or the product. Based on the data table and your initial velocity calculation, the reaction equilibrium is altered more in the direction of the product by the wild-type version. the mutant version. neither version. both versions.
Transcribed Image Text:Calculate the initial velocity of the reaction catalyzed by the wild-type enzyme when the substrate concentration is 10 mM. -1 Vo µmol min The reaction equilibrium is reached once there is no net change in the concentration of the substrate or the product. Based on the data table and your initial velocity calculation, the reaction equilibrium is altered more in the direction of the product by the wild-type version. the mutant version. neither version. both versions.
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