You are provided with a stock standard with a concentration of 100 mg/L, and a solution of unknown concentration. Prepare calibration standards using a 10 mL volumetric flask and a 1 mL piston pipette, and measure on the UV/Vis spectrophotometer (400 nm). Determine the concentration of the unknown after a 1:40 dilution, and provide the concentration of the initial solution. i need to learn how to do the calculations and draw a calibration curve to find concentration of unknown sample after i find the absorbances.
You are provided with a stock standard with a concentration of 100 mg/L, and a solution of unknown concentration. Prepare calibration standards using a 10 mL volumetric flask and a 1 mL piston pipette, and measure on the UV/Vis spectrophotometer (400 nm). Determine the concentration of the unknown after a 1:40 dilution, and provide the concentration of the initial solution. i need to learn how to do the calculations and draw a calibration curve to find concentration of unknown sample after i find the absorbances.
Chapter27: Molecular Fluorescence Spectroscopy
Section: Chapter Questions
Problem 27.9QAP
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You are provided with a stock standard with a concentration of 100 mg/L, and a solution of unknown concentration. Prepare calibration standards using a 10 mL volumetric flask and a 1 mL piston pipette, and measure on the UV/Vis spectrophotometer (400 nm). Determine the concentration of the unknown after a 1:40 dilution, and provide the concentration of the initial solution.
i need to learn how to do the calculations and draw a calibration curve to find concentration of unknown sample after i find the absorbances.
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