Answered: You are designing a phage therapy for a…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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You are designing a phage therapy for a cystic fibrosis patient with an multi-antibiotic resistant Mycobacterium infection. The Mycobacterium infection is preventing the patient from taking immunosuppressant drugs that are needed for a successful lung transplant. The idea is that introducing the right phage to the patient will kill the Mycobacterium cells and allow the patient to go on immunosuppressant drugs that will ensure the new lung is not rejected by the body.

You need to find a phage that infects the Mycobacterium you isolated from the patient.

Which TWO options have the highest chance of success?

Group of answer choices

1.Isolating new phage from soil using a plaque assay with the Mycobacterium isolate

2.Choosing likely candidates from analysis of phage 16S rRNA phylogeny in the tree of life

3.Obtaining a very well-studied phage, such as T4 bacteriophage, that specifically targets E. coli

4.Screening a colleague's library of known Mycobacterium phages for phage that infect the patient's Mycobacterium isolate

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