Why is 80% ethyl alcohol is considered as the solvent of choice during phytochemical screening? What are the different types of extraction? Differentiate each. Differentiate Primary versus Secondary Metabolites and give examples.
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Answer the following questions.
- Why is 80% ethyl alcohol is considered as the solvent of choice during phytochemical screening?
- What are the different types of extraction? Differentiate each.
- Differentiate Primary versus Secondary Metabolites and give examples.
- How are flowers, fruits and seeds dried?
- Explain why flowers requires greater care in drying.
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- Explain the function of BAP in the preparation of plant tissue culture media.What is the importance of the following components of Hay Infusion-paramecium sp culture? a. hay leaves b. pond water/ river water/ estero water c. oxygen1a)A surface sterilisation protocol was carried out to sterilise leaf and stemexplants for evaluating the efficacy of the protocol. All explants were washedthoroughly with distilled water before treating with 50% Clorox for 20minutes. The explants were then rinsed with distilled water before the explants were cultured on plates containing Murashige and Skoog (MS) basal medium. A total of five plates were prepared for each type of explant and each plate consisted of 10 explants. The results recorded after a week are shown in Table. Discuss and conclude the results obtained.
- In phytochemical determination of natural products, one of the tests involve determination of indoles. Would the presence of plant secondary metabolites interfere with the tests for proteins giving false-positive results?Using a batch culture method, the effects of 3 types of media on the growth of a plant suspension culture was investigated. Figure below has shown the growth of Murashige and Skoog (MS), Gamborg B5, and Vacin and Went media in plant suspension cultures. Discuss on the findings and draw conclusions. With explanation, recommend which medium is best for maintaining suspension culturesExplain why micropropagation of banana is a better way of producing planting materials than traditional methodologies. Describe how to carry out micropropagation for this particular crop, including the type of explant to be used and the use of phytohormones or additives in the culture medium needed for each culture stage, along with justifications.
- 1(a) 1. What is the theory upon which intermittent sterilization is based? (B) Is dry or moist heat more efficient as a sterilizing agent? Why? (C) When would it be necessary to use aseptic techniques when inoculating a tube of nutrient broth with dirt? (D)Why do some microorganisms characteristically form a growth of the pellicle type? What environmental factors might alter the formation of a pellicle? (This is not a graded assignment)what is the chemical name , WSSA Mode of Action # , Mode of Action group name , How does the chemical Kill plant (short sentence) , is it Soil-applied or Foliar applied . For the following herbicides : Atrazine , Dicamba , 2 4-D , Pendimethalin , Halosulfurln , Imazapic , Imazethapyr , Sethoxydim , Paraquatdistinguish between selective, differential, enriched, and minimal media. Give examples of Distinguish between selective, differential, enriched, and minimal. Give examples of each. know on what the selective and differential properties are based.
- Phytomenadione MEDICATION IN EACH CATEGORY OF DRUGS AND APPLY THE 10 R’S TO MEDICATION Fill out all the the blanks in the table below. CATEGORY 2 : DRUGS USED FOR PEDIA PATIENTS ( Phytomenadione) 10 R’s TO MEDICATION BRIEF DISCUSSION OF EACH OF THE 10R’s TO MEDICATION APPLICATION (Phytomenadione) 1. RIGHT DRUG (Phytomenadione) 2. RIGHT DOSE 3. RIGHT TIME 4. RIGHT ROUTE 5. RIGHT PATIENT 6. RIGHT TO EDUCATE 7. RIGHT TO REFUSE 8. RIGHT ASSESSMENT 9. RIGHT EVALUATION 10. RIGHT DOCUMENTATIONWith aid of a component layout diagram, describe the principle of operation of little sister sterilizer.Why does turbidity lose reliability at high cell concentrations when the culture reaches the stationary phase?