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- Most of the ultraviolet absorption of proteins at 280 nm is due to their content of a. Glutamate b. Tryptophan c. Alanine d. Aspartate4) Paxlovid is treatment for COVID-19 that was recently approved for use because it can drastically reduce the risk for hospitalization and death when taken early in the infection. It is actually a package of two different antiviral drugs. One of those is Nirmatrelvir, which is a modified tripeptide protein mimetic designed to covalently bind to and inhibit the main protease (MPro) from the SARS-CoV-2 virus. This protease recognizes and hydrolyzes peptide bonds at specific points in their sequence, and it is essential for viral replication. a. Below is one of the sequences that Mpro recognizes and cleaves. It cleaves the peptide bond between the glutamine and the glycine. Draw out the structure of a peptide with this sequence, including the appropriate stereochemistry for biological amino acids and assuming the peptide is at physiological pH (pH = 7.4). SGVTFQGKF b. What would the net charge of the peptide be at physiological pH?. c. At what pH would the peptide be net neutral?Temperature 4 °C 21 °C 30 °C 37 °C 90 °C Absorbance at zero min 1.300 0.952 0.876 0.914 0.903 Absorbance after 10 min of incubation 1.160 0.743 0.042 0.016 0.758 Aomin – A10min 0.140 0.209 0.834 0.898 0.145 Enzyme activity in 10 min (umoles) Enzyme activity (umoles pyruvate/min) CALCULATIONS: show calculations Where extinction coefficient is 6.22 x 103
- Q12 and Q13 and Q 14 pleeeeeeeeeease"The coronavirus disease (COVID-19) is an infectious disease caused by a new strain of coronavirus. This new virus and disease were unknown before the outbreak began in Wuhan, China, in December 2019. On 30 January 2020, the Philippine Department of Health reported the first case of COVID-19 in the country with a 38-year-old female Chinese national. On 7 March, the first local transmission of COVID-19 was confirmed. WHO is working closely with the Department of Health in responding to the COVID-19 outbreak." Aki and her family were alarmed with the news they watched and so first thing in the morning they rush to the nearest convenient store to secure disinfectant and sanitizers but unfortunately the store already had empty shelves of the essentials they needed. They went to other stores searching and to their dismay they acquired nothing. They were on their way home when she suddenly remembered her past lesson on "solutions", and so she immediately ran back to the store and purchase…You performed a kinetic study of the enzyme lipase and you obtained the following double reciprocal (Lineweaver-Burk) plot: 4 y = 4.3511x + 1.8889 %3D 1 -0.5 -0.3 -0.1 0.1 0.3 0.5 0.7 0.9 1/[S] When you performed the same kinetic study in the presence of an inhibitor, you obtained a new line that had y-intercept 3 and x-intercept -0.3. Based on this information select all correct statements about this inhibitor. This is mixed inhibitor. When this inhibitor is present apparent Km of enzyme decreases. This inhibitor prevents substrate from binding to the enzyme. This is uncompetitive inhibitor. When this inhibitor is present apparent Vmax of enzyme decreases. When this inhibitor is present apparent Km of enzyme increases. This inhibitor can bind to either enzyme or enzyme-substrate complex.
- Situation: “The coronavirus disease (COVID-19) is an infectious disease caused by a new strain of coronavirus. This new virus and disease were unknown before the outbreak began in Wuhan, China, in December 2019. On 30 January 2020, the Philippine Department of Health reported the first case of COVID-19 in the country with a 38-year-old female Chinese national. On 7 March, the first local transmission of COVID-19 was confirmed. WHO is working closely with the Department of Health in responding to the COVID-19 outbreak.” Aki and her family were alarmed with the news they watched and so first thing in the morning they rush to the nearest convenient store to secure disinfectant and sanitizers but unfortunately the store already had empty shelves of the essentials they needed. They went to other stores searching and to their dismay they acquired nothing. They were on their way home when she suddenly remembered her past lesson on “solutions”, and so she immediately ran back to…Situation: “The coronavirus disease (COVID-19) is an infectious disease caused by a new strain of coronavirus. This new virus and disease were unknown before the outbreak began in Wuhan, China, in December 2019. On 30 January 2020, the Philippine Department of Health reported the first case of COVID-19 in the country with a 38-year-old female Chinese national. On 7 March, the first local transmission of COVID-19 was confirmed. WHO is working closely with the Department of Health in responding to the COVID-19 outbreak.” Aki and her family were alarmed with the news they watched and so first thing in the morning they rush to the nearest convenient store to secure disinfectant and sanitizers but unfortunately the store already had empty shelves of the essentials they needed. They went to other stores searching and to their dismay they acquired nothing. They were on their way home when she suddenly remembered her past lesson on “solutions”, and so she immediately ran back to…Situation: “The coronavirus disease (COVID-19) is an infectious disease caused by a new strain of coronavirus. This new virus and disease were unknown before the outbreak began in Wuhan, China, in December 2019. On 30 January 2020, the Philippine Department of Health reported the first case of COVID-19 in the country with a 38-year-old female Chinese national. On 7 March, the first local transmission of COVID-19 was confirmed. WHO is working closely with the Department of Health in responding to the COVID-19 outbreak.” Aki and her family were alarmed with the news they watched and so first thing in the morning they rush to the nearest convenient store to secure disinfectant and sanitizers but unfortunately the store already had empty shelves of the essentials they needed. They went to other stores searching and to their dismay they acquired nothing. They were on their way home when she suddenly remembered her past lesson on “solutions”, and so she immediately ran back to…