When C-2 is labeled, why does the label survive two full turns of the cycle but then become equally distributed among all four carbons of oxaloacetate by the end of the second cycle? (a) Fate of the carboxyl carbon of acetate unitHO-OxaloacetateMalateFumarateOxaloacetateHO-O00MalateFumarateSuccinate6-7710-4733Malate49OxaloacetateFumarateSuccinyl-CoA(b) Fate of methyl carbon of acetate unitO-S-ColSuccinate-S-CoAHO-O1st turn1st turnSuccinateHO-CCitrateHO-3rd turnSuccinyl-CoAHO-a-ketoglutarateCitrateIsocitrateно-a-ketoglutarateCitrateSuccinyl-CoAIsocitrateHO-Isocitratea-ketoglutarateO(100₂)O00₂)(00₂)(CO₂)(00₂)(CO₂)½ Totalmethyl ClabelOOxaloacetateHO-O-OMalateFumarateHO-G0-7-7MalateOxalcacetateFumarate0-8-5-coHO-923Malate29SuccinateFumarate02999Oxaloacetate2nd turnHO-0-8--0SuccinateSuccinyl-CoA2nd turn110-Succinate0-8-s-cCitrate10-4th turnSuccinyl-CoAa-Ketoglutarate,CitrateHOIsocitrateHO-a-KetoglutarateSuccinyl-CoACitrateHOIsocitrate O(100₂)Isocitratea-Ketoglutarate(00₂)(CD)(00₂)↑(CO₂),All labeledcarboxyl carbonremoved by thesetwo stepsTotalmethyl Clabel

Oxaloacetate is a four-carbon compound, and carbon-2 (C-2) refers to the second carbon atom in the…

Answered: When C-2 is labeled, why does the label…

When C-2 is labeled, why does the label survive two full turns of the cycle but then become equally distributed among all four carbons of oxaloacetate by the end of the second cycle?

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

When C-2 is labeled, why does the label survive two full turns of the cycle but then become equally distributed among all four carbons of oxaloacetate by the end of the second cycle?

(a) Fate of the carboxyl carbon of acetate unit
HO-
Oxaloacetate
Malate
Fumarate
Oxaloacetate
HO-O
00
Malate
Fumarate
Succinate
6-77
10-47
33
Malate
49
Oxaloacetate
Fumarate
Succinyl-CoA
(b) Fate of methyl carbon of acetate unit
O-S-Col
Succinate
-S-CoA
HO-O
1st turn
1st turn
Succinate
HO-C
Citrate
HO-
3rd turn
Succinyl-CoA
HO-
a-ketoglutarate
Citrate
Isocitrate
но-
a-ketoglutarate
Citrate
Succinyl-CoA
Isocitrate
HO-
Isocitrate
a-ketoglutarate
O
(100₂)
O
00₂)
(00₂)
(CO₂)
(00₂)
(CO₂)
½ Total
methyl C
label
O
Oxaloacetate
HO-O-O
Malate
Fumarate
HO-G
0-7-7
Malate
Oxalcacetate
Fumarate
0-8-5-co
HO-9
23
Malate
29
Succinate
Fumarate
029
99
Oxaloacetate
2nd turn
HO-
0-8--0
Succinate
Succinyl-CoA
2nd turn
110-
Succinate
0-8-s-c
Citrate
10-
4th turn
Succinyl-CoA
a-Ketoglutarate,
Citrate
HO
Isocitrate
HO-
a-Ketoglutarate
Succinyl-CoA
Citrate
HO
Isocitrate O
(100₂)
Isocitrate
a-Ketoglutarate
(00₂)
(CD)
(00₂)
↑
(CO₂),
All labeled
carboxyl carbon
removed by these
two steps
Total
methyl C
label

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