What type of growth is shown on this graph? YEAST POPULATION GROWTH IN A SUGAR SOLUTION OVER A 20-HOUR PERIOD 75 30 15 12 14 16 18 20 Hours density-independent average O logistic O exponential Amount of yeast (mgioc
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- Yeast cells has been cultured on glucose (Table 1). The growth data follows the Monod Equation Table 1: Growth data for yeast cells Glucose concentration (mg/L) 7 10 15 40 200 1000 Specific growth rate (h*¹) 0.066 0.088 0.12 0.294 0.330 0.364 0.419 0.451 0.2 0.304 0.340 0.458 Observed yield, Y'x/s (g/g) a) Calculate the maximum specific growth rate (max) and the substrate constant (Ks) b) Estimate the true yield Y'x/s and the maintainance coefficient ms c) Calculate the doubling time at a concentration of 15 mg/L glucose d) Calculate the substrate consumption rate (rs) assuming a substrate concentration of 40 mg/L and a biomass concentration of 100 mg/LThe following four chemicals commonly found in defined growth media contain all of the necessary atomic macronutrients, namely C, O, N, H, S and P, which microbes require: glucose (C6H12O6), K2HPO4, MgSO4.7H2O, and (NH4)HPO4. Part a. Create a simple diagram to map which growth media chemical contributes atoms to which of the four biological macromolecules (amino acids, polysaccharides, lipids, and ribonucleic acids). Part b. Consider a cell culture broth that originally (before inoculation with cells) contains 5.0 gr/L glucose (C6H12O6), 1.2 gr/L K2HPO4, 0.3 gr/L MgSO4∙7H2O, and 1.0 gr/L (NH4)HPO4. Create a table showing how much of each of the different atoms (C, H, O, K, P, Mg, S, N) are available for incorporation into cell macromolecules (assume 50% of the glucose is catabolized, while the remainder goes for energy (respiration). Express your answers with units of grams of atomic species / liter of culture broth.The chemical reaction equation for the conversion of ethanol (C2H5OH) to acetic acid (CH3COOH) is: C2H5OH + O2 → CH3COOH + H2OIn a vinegar production industry, acetic acid is produced from ethanol during the growth of the bacterium Acetobacter aceti, whose cellular composition is CH1,8O0,5N0,2. The biomass yield from substrate is 0.14 g/g, while the product yield also from substrate is 0.92 g/g. Ammonia is used as a nitrogen source. Based on this information, answer: (a) What is the oxygen demand for acetic acid production in this crop?(b) What are the cooling requirements of a bioreactor containing 3000 L of ethanol (ρ = 789 kg/m³), to be completely converted into acetic acid?
- AaBbCcDd AaBbCcDd AaBbC AaBbCcC AaB AaBbC I Normal 1 No Spac. Heading 1 Heading 2 Title Subtit Paragraph Styles BIO 121 Section Yeast cells use sugars to undergo the chemical reactions of cellular respiration. We will test the ability of yeast cells to sucrose as an energy source for cellular respiration. We will examine if various concentrations of sucrose has an effect on cellular respiration and whether the temperature also plays a role in cellular respiration Answer these questions based on the video posted: Using this data table graph the data from the experiment in the video: Amount of Foam in the Yeast Experiment| Time 20°C/RT 30°C 40°C 50°C 60°C O min Ост Ocm Ocm Ост Ост 10 min 1.5cm 4cm 11cm бст Зст 20 min 2.5cm 9.5cm 17cm 11cm 9cm 30 min 4.5cm 13cm 18cm 10cm 11cm Using the data presented in the video for the yeast experiment draw a line graph. There are time points shown 0, 10, 20 and 30 minutes. Plot a line graph of foam level(s) for each temperature versus time (Time is on…At what sucrose molarity does the trend line cross the 0% difference point on the graph ? A microorganism is grown in a fermenter under steady-state conditions. The microorganism growth follows Monod kinetics and the following stoichiometric equation: C3H6O3 +a O2 +b NH3 → c biomass + d CO2 + e H₂O Given that the biomass yield is 0.415 grams of biomass per gram of substrate, determine the respiratory quotient.
- A batch reactor was initially filled with organic substrate (S0) with an equivalentconcentration of COD = 1000 mg/L and 100 mg/L of bacterial biomass (X0). After 24 hraeration, the biomass in the reactor increased to 300 mg/L, while the substrateconcentration decreased to 200 mg/L as of COD; what is the observed biomass yield (Y)during this biodegradation process?Two microorganisms that use naphthalene as a food source have the same Umax but different Ks values for naphthalene. If both types of microorganisms are inoculated at the same cell density into a liquid culture containing naphthalene as the sole carbon source (i.e. the only food source), which microorganism will outcompete the other: the one with the higher Ks or the one with the lower Ks. Why?.The data below were obtained for the growth of a pure culture of Escherichia coli in nutrient broth at a temperature of 37°C. Determine (1) the specific growth rate and (2) generation time of E. coli and the duration of the (3) lag and (4) log (or exponential) phases. (ln 2 = 0.693) Time (h) 0 1 2 3 4 8 16 32 Bacterial No./mL 104.1 103.9 104.4 105.5 106.5 107.7 108.0 107.6
- Yeast cells grew from 19 to 54 kg dry cells m-3 in 7 hours. During this period 81 g of glycerol was consumed per 1L of the fermentation broth. Determine the average specific growth rate and the cell yield with respect to glycerol.Consider the growth curve shown below. Cells were grown in a medium containing 1 % w/v glucose and 0.5% w/v acetate. Calculate the generation time when growing on glucose and also when growing on acetate. Pay particular attention to the split time axis with different time scales. Glucose generation time = hrs Acetate generation time = hrs 1011 1010 109 108 107 106 105 104 10 15 20 25 30 time (hours) HA log of numbers of bacteria LO39. Tetrahymena thermophili protozoa have a doubling time of 6.5 hrs. when grown using bacteria as a limiting substrate. The protozoa are cultured at steady state in a chemostat using a feed stream containing non-viable bacteria using a feed stream containing non- viable bacteria. The concentration of the non-viable bacteria in the culture volume is 10g/L. If the substrate concentration for protozoa to convert the substrates at half their maximum rate is 12mg/L, what would be the maximum dilution rate for operation of the chemostat at a quasi-steady state? Assume no products are formed and protozoan cell death and maintenance requirements are negligible.