Q: Identify The most commonly used stationary phase in paper chromatography.
A: The question is based on the concept of chromatography. It is a technique of separation and…
Q: Which of the following factors DOES NOT affect retention time of a sample undergoing normal phase…
A:
Q: What would happen if we use longer columns and select adsorbing materials with 200-400 mesh sizes…
A: Solution is given in the following steps:
Q: Are the number of spots shown on SDS-PAGE electrophoresis dependant on whether the protein is a…
A: It is to explain that whether the number of spots shown on SDS-PAGE electrophoresis is dependent on…
Q: What are the limitations of using molecular/size exclusion chromatography?
A: In SEC, mass is not measured so much as the hydrodynamic volume of the polymer molecules, that is,…
Q: Capillary electrophoresis differs from other chromatographic methods because:
A: It uses unique detectors.
Q: what is the difference between liquid phase extraction and solid phase extraction
A: A question based on analytical process that is to be accomplished.
Q: What are the different technical considerations in performing the serum protein electrophoresis…
A: Answer - The serum protein electrophoresis (SPEP) test measures specific proteins in the blood to…
Q: How is matrix selected in soft ionization? Give three examples.
A: Soft ionization is a type of ionization where a laser strikes a matrix of small molecules convert…
Q: Is it possible to detect metal complexes of organic acids with thin layer chromatography?
A: Thin layer chromatography, abbreviated as TLC, is used to identify the element or compound present…
Q: What is the impact of the shape of the solvent front in CE (Capillary Electrophoresis) compare to…
A: Capillary electrophoresis (CE) is an analytical technique that separates ions based on their…
Q: Differentiate between isocratic elution and gradient elution. Why is gradient elution sometimes a…
A:
Q: In reverse phase chromatography which analyte will elute first, most polar or least polar?
A: In reverse phase chromatography, polar solvent is used as a mobile phase.
Q: How do quenchers affect the duration of chemiluminescence reactions?
A: The presence of quencher retards the excitation of compound from its singlet state to triplet state.…
Q: What are the advantages of using a multidimensional HPLC separation for the analysis of complex…
A: Since you have asked multiple questions, we will solve the first one for you. For remaining…
Q: What is the difference between Mohr and serological pipette?
A: Difference between Mohr and serological pipette is given below.
Q: The plot of retention time versus detector signal in chromatography called?
A: In the GC-plot, the detector response signal is at the y-axis, and the retention time is at the…
Q: What kind of samples can be analyzed by AFM? What are the applications of AFM?
A: Atomic Force Microscopy (AFM) is a powerful technique that can image virtually any type of surface,…
Q: Why does the efficiency (decreased plate height) of liquid chromatography increase as the stationary…
A: to improve resolution in HPLC by Increasing column length. by Decreasing particle size. by…
Q: Differentiate between gel filtration and gel permeation chromatography.
A: Given : We have to tell what is the difference between gel filtration and gel permeation method.
Q: Analysis of protein purity using two-dimensional electrophoresis could involve
A:
Q: 3 Compare: a) Capillary Electrophoresis with GC and HPLC. b) Separation Versus Preconcentration.
A:
Q: ) -SH -OCH₂ -NO₂ silica column 20% CHCI₂ 6 Time (min) 8 -CIN 10 -OH -CO₂CH₂-COCH, CHO 12 -NH₂ 400…
A: Silica Column is eluted by 29% CHCl3. Now in the given graph elution time of different compounds are…
Q: Explain the term gradient elution mode in HPLC
A: Given : We have to tell about gradient elution term .
Q: What are the main differences in column size, operating pressure, sample volume and run time between…
A: Chromatography is a technique which is used for separating the components, or solute of a mixture…
Q: What is electrophoresis due to?
A: Interpretation: What is electrophoresis due to?
Q: what are advangtage and disadvantage of using an Atomic Force Microscopy and explain each
A: Atomic force microscopy is a technique by which we study the different type of surfaces like…
Q: Peak Retention Time (minutes) Area 1.201 136501204 acetone 2.011 1253056 toluene ortho-product…
A:
Q: 11. (8pts) how can silica gel Fsa TLC plates can be visualized ( give two methods? :
A: In the given question we have to visualise the F254 TLC plate, there are two method by which we can…
Q: Why does a molecularly imprinted polymer selectively bind a desired analyte?
A: Molecularly imprinted polymer selectively bind a desired analyte has to be explained.
Q: In general, increasing the molecular weight of a Gd-based MR contrast agent results in an increase…
A: True. The proton relaxivity of a Gd(III) compound is determined by several factors and can be varied…
Q: What properties should the stationary-phase liquid for gas chromatography possess?
A: Desirable properties of the stationary phase for GC include low volatility, thermal stability,…
Q: The total retention time of dodecane on a 15 m x 0.25 mm i.d. DB-5 GC column was 8.0 min. The column…
A: Given: Number of theoretical plates=N=25600Retention time=tR=8.0 min
Q: The resulting retention volumes for a series of polystyrene standards Molar mass (g/mol) Retention…
A: Given: Molar mass (g/mol) log(Mw) Retention volume (mL) 640000 5.80618 12.14 483000 5.683947…
Q: What is Jon chromatographys
A: Given To know about the chromatography
Q: What is the retention factor of a sample when after injectiion the void time (tM) took 4.18 sec? The…
A:
Q: Describe the following gel matrices used in gel filtration chromatography and discuss the types of…
A:
Q: Z and E -oct-3-en-2-ol is hard or easy to separate in thin layers in chromatography?
A: Greater the polarity difference between the two components of a mixture easier will be the…
Q: The particle size of the silica gel influences the quality of the separation in column…
A: The column chromatography involves the separation of a single compound from the mixture of fluid.…
Q: What is stationary phase used is liquid chromatography? What compounds does it bind best and why?
A: Liquid chromatography is a separation technique on the basis of colour in which the mobile phase is…
Q: How does capillary zone electrophoresis (CZE) works.
A: Capillary zone electrophoresis is a technique used for separating the individual components from a…
Q: Gel permeation chromatography depends on the degree of species segregation of a sample
A: Given Gel permeation chromatography depends on the degree of species segregation of a sample…
Q: In thin layer chromatography what is the mobile phase?
A: Chromatography is a technique used for the separation of non-volatile liquids.
Q: How is matrix selected in soft ionization? Give examples.
A: In mass spectrometry soft ionization techniques are used to observe and analyse mainly the molecular…
Q: Name types of gels (sieving matrix) that may be used for gel filtration chromatography and discuss…
A: The types of gels for gel filtration chromatography include, Dextran Polyacrylamide beads/ agarose…
What is 2D gel electrophoresis
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- What kind of samples can be analyzed by AFM? What are the applications of AFM?Whose method should be used for the mass production of black ink? What is the retention factor (Rf) of the most polar and least polar spot present in the standard ink?A thermal conductivity detector (TCD) is used in a) HPLC b) GC c) size exclusion chromatography d) capillary electrophoresis
- A gel filtration column has a radius (r) of 0.80 cm and a length (l) of 20.0 cm. Calculate the total volume of the column, which is equal to pr2l.Why chromatography is an important tool for analysis of organic compounds?Can you write a report on protein electrophoresis Q&A please Title: Protein Electrophoresis Aim of Experiment: Determination of individual protein in serum
- 29. What exactly is relative retention and retention time? How do you solve problems like this?A supporting electrolyte is usually added to the analyte solution to minimize solution resistance.minimize solution conductivity.make the analyte less soluble in solution.increase the scan rate.What is the stationary phase in thin layer chromatography and its polarity? Choices: Cellulose - non-polar Cellulose - polar Silica - non-polar Silica - polar