What are the disadvantages of using the mouth in filling a pipette? Why do we use exponential notation in counting bacteria? Why is serial dilution necessary?
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- What are the disadvantages of using the mouth in filling a pipette?
- Why do we use exponential notation in counting bacteria?
- Why is serial dilution necessary?
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- The process of collecting characteristics of your unknown bacterium can be helped by the use of a dichotomous key. A dichotomous key is a flowchart that can be used to identify an organism. a) in your own words explain how to create and use a dichotomous key to identify unknown bacteriaPlease describe the pros and cons of the following methods for counting bacterial cells. 1) Petroff-Hausser counting chamber 2) Coulter Counter 3) Plate Count1) both streak plating and pour plating produce isoluated colonies. What is the underlying explanation for why both methods work; that is, what are both methods doing with repect to the bacterial cells? 2) If streak plates failed to produce isolated colonies, describe two things that you could do to improve your chance of generating isolated colonies. 3) Why do we care so much about producing isolated colonies? what is an isolated colony composed of? What can you do with an isolated colony?
- You take 10 ml of a stock solution, which is at a concentration of 1000 phage/ml, and dilute it to a total of 100 ml. From the resulting solution you take 5 ml and dilute it to 25 ml, and from the latter you take 5 ml and make a total of 20 ml. a) It will be possible to know how many bacteriophage particles there will be in 1 ml of the last solution b) What is the dilution factor in each step, in the same order in which the dilutions are made? c) What is the total serial dilution factor?How would you produce a 10^-1 dilution if a 3 mL bacterial sample using the entire 3 mL volume? suppose your professor handed you a test tube with 2.0 mL of an E. coli broth culture in it and told you to make a 10^-1 dilution of the entire culture. Explain how you would do this. Show your calculations.You perform a ten-fold serial dilution of a bacterial culture to determine the number of colony forming units (CFU) per mL in the culture. You then do a plate count with these growth results (no. of colonies): 1:10: too many to count; 1:100 too many to count; 1:1,000: 126 colonies; 1:10,000 14 colonies; 1:100,000: no growth The number of CFU per mL in the original culture was: 1 ml 1 ml 1 ml 1 ml Original inoculum Dilutions 1,000 126 9 ml broth in each tube 126,000 10,000 140,000 1:10 1 ml 1:100 1 ml 1:1000 1 ml 1 ml 1:10,000 1 ml 1:100,000 1 ml
- You were asked to prepare a dilution series of a bacterial culture where only 3 tubes will be used (all with 5 mL total solution). Draw a schematic diagram to make tube 1 have a 10-2 dilution, tube 2 have 10-3 dilution, and tube 3 to have 10-5 dilution You were instructed to dilute an antibiotic solution 1/10, redilute 1/25, and again 1/50, then you need to make 100mL of each dilution. How would you go about preparing this dilution series? (Present your answer in a schematic diagram)You have used morphological observations and biochemical tests to identify two unknown bacterial isolates in your practical sessions, briefly describe three other techniques that can be used to identify unknown bacteria (you can use any reliable online resources). Note: These must not be based on biochemical tests (enzyme use, acid production etc) or morphological characteristics (size, shape, structures).Use the following passage to answer 2 consecutive questions. A urine specimen was collected in a container and placed on the central desk for delivery at 10:00 am. The specimen is not sent to the lab until 5 hours later (3:00 pm). The sample time was marked by the central clerk as collected at 4:00 pm. The bacterial species has a generation time of 30 minutes at room temperature. There are 100 bacterial cells/ml at the time of collection (10:00 am). The laboratory will cite a bacterial infection at more than 1000 bacterial cells/ml.
- what is the most efficient way to solve this dilution word problem?How can colony morphology be used in the identification of bacterial species? What are the environmental factors that affect the growth of bacteria? What is agar (or agar-agar)? Is this different from nutrient agar? Why? How is xylol used to clean the lens of a microscope? What may happen if xylol is constantly used for this purpose? For what purpose would you adjust each of the following microscope components during a microscopy exercise? iris diaphragm coarse adjustment knob fine adjustment knob condenser mechanical stage controlwhat is the color of the capsule and color of the bacterial cell of streptococcus pneumoniae respectively? what is the color of the capsule and color of the bacterial cell of klebsiella pneumoniae respectively?