What amino acids are possibly present in each sample tests? Explain why.
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Tests for Qualitative Analysis of Proteins and Amino Acids:
- Ninhydrin Test
- Biuret Test
- Xanthoproteic Test
- Millon's Test
- Hopkins-Cole Test
- Sakaguchi Test
- Lead Acetate Test
What amino acids are possibly present in each sample tests? Explain why.
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- What is the color results of tyrosine,phenylalanine,alanine in millon test? Results and reference plsPlease explain the theoretical background (positive color reactions and what is being detected) involved in the different qualitative test for proteins that has been isolated intact or hydrolyzed. Please directly answer the theoretical background (positive color reactions and what is being detected) of each test GENERAL: BIURET - NINHYDRIN SPECIFIC XANTHOPROTEIC TEST - MILLON'S TEST - HOPKINS-COLE TEST - SAKAGUCHI TEST - LEAD ACETATE TEST - NITROPRUSSIDE TEST - FOLLIN'S REACTION - SULLIVAN'S TEST - PAULY REACTION - LIEBERMAN'S TEST -Why glucose (monosaccharide) and maltose (disaccharide) give positive Benedict test?
- Match the objective of the test. -Mucic acid Test -Seliwanoff's Test -Bial's Test -Nylander's Test -Barfoed's Test Detects optical activity of reducing sugar Confirms the presence of pentose Differentiates reducing monosaccharide from reducing disaccharide Detects presence of glucose in urine Confirms presence of polyhydroxyaldehyde Identifies the presence of C=O in ketohexose Detects presence of brain sugar General test for polymers of polyhydroxy aldehyde and ketonesName the reagent test for amino acids and proteinsGive the chemical principles involved in a positive Biuret test, Millon’s Test and Sakaguchi Test. What is the chemical basis of the Ninhydrin Test, Hopkins – Cole test and Sulfur reaction test? What chemical structure should be present in an amino acid or protein to give a positive Xanthoproteic test?
- Qualitative and Quantitative Tests for Amino Acids and ProteinsComplete the table: Test Reagents Positive Result Positive Indication Ninhydrin test Xanthoproteic acid test Millon's test Hopkins Cole test Pauly's diazo Test Lead sulphide test Histidine test Isatin test Folin’s McCarthy Sullivan Test Nitroprusside TestWhy is it not advisable to use adhesive mixture if protein histological investigation are contemplatedWhat chemical structure should be present in an amino acid or protein to give a positive Xanthoproteic test? What chemical test could be used to differentiate a protein from an amino acid? Explain.
- What does translucent spot mean with regards to lipid? Identify the control for this test. 1. Oil 2. Cheese 3. Water 4. CheeseThe table below summarizes the results for Millon's test. Provide the correct remarks from the results if Negative for Millon's Test or Positive for Millon's Test and if Contains phenolic hydroxy group or Does not contains phenolic hydroxy group.concentrated lye has a pH of 7 Bial's test uses concentrated HCI as a dehydrating acid and orcinol + traces of ferric chloride as condensation reagent. The test reagent dehydrates pentoses to form furfural. Furfural further reacts with orcinol and the iron ion present in the test reagent to produce a bluish or green product O red precipitate yellow solution light pink or pink solution Other: In the process of soapmaking, it is important that you add sodium hydroxide slowly to water because set at a hiah temperature