What advantages do cDNA libraries provide over genomic DNA libraries? Describe cloning applications where the use of a genomic library is necessary to provide information that a cDNA library cannot.
Q: What is a database? What types of information are stored within adatabase? Where does the…
A: The complete set of chromosomes present in the organism is known as the genome. Bioinformatics…
Q: Which of the following cloning vectors have the minimal features required for cloning ? Answer =
A: Answer :: Brief description about cloning Cloning is a process that allows scientists to create…
Q: Starting with a sample of RNA that contains the mRNA for theβ-globin gene, explain how you could…
A: DNA is the genetic material that carries genetic information in the form of coded nucleotide…
Q: What is an advantage of making a cDNA library rather than a genomic library?
A: DNA library or the gene library is the collection of DNA sequences from different organisms. These…
Q: Diagram a method for creating a cDNA library
A: cDNA library ( COMPLEMENTARY DNA) It is a combination of cloned cDNA fragments which is inserted…
Q: What can serve as footprints to identify genome sites that are or have been occupied by transposable…
A: A genome is a collection of an organism's genetic information.
Q: Why do geneticists studying eukaryotic organisms often construct cDNA libraries, whereas…
A: A cDNA (complementary deoxyribonucleic acid) library is a combination of cloned cDNA fragments…
Q: List three independent techniques you could use toidentify DNA sequences encoding human geneswithin…
A: A genome is referred to as genetic material of the organism that composed of DNA or RNA. The genome…
Q: A DNA library is a collection of clones, each containing a different fragment of DNA, inserted into…
A: According to the question, A DNA library is a collection of clones, each containing a different…
Q: Why is cDNA used for cloning?
A: Cloning is the production of organisms, which are genetically identical to their parents. These…
Q: How are probes used to screen DNA libraries? Explain how a synthetic probe can be prepared when the…
A: A probe is an oligonucleotide stretch of DNA (Deoxyribonucleic Acid) or RNA (Ribonucleic Acid) which…
Q: What major advantage does cloning mammalian genes frommRNA or using synthetic genes have over PCR…
A: Cloning by using mRNA requires DNA with desirable genes obtained using restriction enzymes which are…
Q: If I clone a complete eukaryotic gene, including the eukaryotic promoter region, ligate it into a…
A: Transformation is the process in which a bacterium takes up DNA from the environment, that has been…
Q: What is a cDNA library, and for what purpose can it be used?
A: In genetics, the term cDNA library refers to the library containing the group of cloned copies of…
Q: What types of cells are used for cloning rDNA?
A: rDNA is called recombinant DNA which contains DNA from multiple sources. Recombinant DNA is it's the…
Q: What is RNA sequencing ? Why it is important ?
A: The biochemical material that is carried from the preceding generation to the succeeding generation…
Q: Briefly explain how synthetic probes are created to screen a DNA library when the protein encoded by…
A: Synthetic probe is fragment of DNA. to screen a DNA library RNA of variable length approximately 100…
Q: what are similarities and differences between chain-termination and reversible terminator…
A: Gene sequencing aids in the development of gene therapy, a form of treatment that replaces faulty…
Q: Although a number of different animals have been successfully cloned, the process of creating cloned…
A: A living organism, which is genetically identical to that of the other organism is known as a Clone.…
Q: You are trying to study the effects of Drug A on the expression of Gene X in a tumor sample (lane…
A: Reverse transcription enzyme chain reaction (RT-PCR) may be a variation of normal PCR that involves…
Q: What are the advantages of Next Generation Sequencing?
A: DNA sequencing is a biochemical method for determining the order of nucleotide bases, A, G, C, and…
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A: Essential elements of plasmid cloning vector Presence of Origin of replication for autonomous…
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A: The following is the DNA information received by gel electrophoresis: In lanes 1 and 2, each…
Q: What are the main differences between whole genome sequencing and whole exome sequencing?
A: whole genome sequencing is sequencing the entire genome of the organism, where as whole exome…
Q: Explain about preparation of cDNA libraries ?
A: cDNA (short for complementary deoxyribonucleic acid) is produced from messenger RNA in a process…
Q: "Complementary DNA (cDNA) libraries offer certain advantages over genomic libraries". Explain how ?
A: Introduction The cDNA library is a collection of mRNA segments cloned into independent vector…
Q: Consider the following human genetic diseases: hemophilia, Down syndrome, cystic fibrosis, and brain…
A: CRISPR(Clustered Regularly Interspaced Short Palindromic Repeats) Cas genome editing technology…
Q: You have two samples you have to sequence: one is a cloned plasmid that you want to verify the…
A: Sequencing of DNA refers to the biochemical methods for determining the order of the nucleotide…
Q: Suppose that a human genomic library is prepared by exhaustive digestion of human DNA with the Eco…
A: The human genome project was started in the year 1990, the main objective of this genome project is;…
Q: Suppose that a human genomic library is prepared by exhaustive digestion of human DNA with the EcoRI…
A: No, because most humans genes are much longer than 4kb.
Q: CRISPR One of the more recent advances in biotechnology is the development of the CRISPR gene…
A: Given information: The CRISPR technology is the most recent advancement in the field of…
Q: After Create a second cDNA strand complementary to the first. After the reaction is completed, the…
A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…
Q: In large-genome sequencing projects, the initial data usually reveal gaps where no sequence…
A: Primer walking or directed sequencing is a sequencing method for sequencing DNA fragments that are…
Q: CRISPR RNA (crRNA) in a bacterial cell: is a snippet of genetic material from a virus stored in a…
A: The CRISPR/Cas9 or clustered regularly interspaced short palindromic repeats and CRISPR-associated…
Q: Suppose you were interested in generating a PCR amplicon including the bracketed sequence below.…
A: PCR or Polymerase Chain Reaction is a specialized type of method which is used to obtain copies of…
Q: In your own words, describe the series of steps necessary to clone a gene.
A: Cloning is the process of producing genetically identical copies of an individual by natural or…
Q: List the steps to make a genomic library. What steps would change if you wanted to make a cDNA…
A: You have asked two questions. The first one has been answered. To get the answer for the second…
Q: What is an STS? How are STSs generated experimentally? What are the uses of STSs? Explain how a…
A: BASIC INFORMATION GENE MAPPING This is a technique to identify the position of elements in the…
Q: PCR errors during library amplification are one possible source of false positive results. If an…
A: PCR is a polymerase chain reaction. This is a biotechnology used to amplify the DNA copies.
Q: Why is genome editing by CRISPR-Cas advantageous over traditionalmethods for creating knockout or…
A: Two crucial components make up the CRISPR-Cas9 system, which modifies DNA. These include the Cas9…
Q: List the advantages and disadvantages of using plasmids as cloning vectors. What advantages do BACs…
A: Cloning vectors are small DNA pieces that are maintained stably in an organism. This vector can a…
What advantages do cDNA libraries provide over genomic DNA
libraries? Describe cloning applications where the use of a
genomic library is necessary to provide information that a cDNA
library cannot.
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- Describe the difference between Sanger based sequencing and Next Generation Sequencing (NGS). Why is NGS advantageous over Sanger based sequencing?Briefly explain how synthetic probes are created to screen a DNA library when the protein encoded by the gene is known.A DNA library is a collection of clones, each containing a different fragment of DNA, inserted into a cloning vector. What is the difference between a cDNA library and a genomic DNA library?
- List the steps to make a genomic library. What steps would change if you wanted to make a cDNA library instead?Transcriptome analysis involves two separate methodologies: gene expression and RNA seq analyses. The 10 items below are a scrambled listing of the steps used in the two procedures. Identify the steps involved in RNA seq from the list below. Use the numbers in the list to refer to each step. Once the steps for RNA seq have been identified, write the steps in the order in which they are performed during the experiment. (1) DNA sequencing (2) Allow for hybridization and wash excess cRNA. (3) Mix labeled cRNA with array chip. (4) PCR amplification (5) Measure fluorescence intensity to determine abundance of transcripts. (6) Add labeled cRNA at each microarray location. (7) Map cDNA sequences to the genome of the organism to determine identity and abundance of transcripts. (8) mRNA isolation from cells (9) Prepare fluorescently labeled cRNA probes (10) cDNA synthesisThe technique of fluorescence in situ hybridization (FISH) is described. This is another method for examining sequence complexity within a genome. In this method, a DNA sequence, such as a particular gene sequence, can be detected within an intact chromosome by using a DNA probe that is complementary to the sequence.For example, let’s consider the β-globin gene, which isfound on human chromosome 11. A probe complementary to theβ-globin gene binds to that gene and shows up as a brightly colored spot on human chromosome 11. In this way, researchers can detectwhere the β-globin gene is located within a set of chromosomes. Becausethe β-globin gene is unique and because human cells are diploid(i.e., have two copies of each chromosome), a FISH experimentshows two bright spots per cell; the probe binds to each copy ofchromosome 11. What would you expect to see if you used thefollowing types of probes?A. A probe complementary to the Alu sequenceB. A probe complementary to a tandem array near…
- Whole-exome sequencing (WES) is helping physicians diagnose a genetic condition that has defied diagnosis by traditional means. The implication here is that exons in the nuclear genome are sequenced in the hopes that, by comparison with the genomes of nonaffected individuals, a diagnosis might be revealed. (a) What are the strengths and weaknesses of this approach? (b) If you were ordering WES for a patient, would you also include an analysis of the patient’s mitochondrial genome?Human genomic libraries used for DNA sequencing are often made from fragments obtained by cleaving human DNA with Haeiii in such a way that the DNA is only partially digested; that is, not all the possible HaeIII sites have been cleaved. What is a possible reason for doing this?You are trying to study the effects of Drug A on the expression of Gene X in a tumor sample (lane 3). You perform an RT- PCR reaction using a set of prímers to amplify a region of Gene X that is about 600bp in length. The forward and reverse primers are on 2 separate exons. You run your target CDNA fragment on an agarose gel (lane 3). Lanes 1 and 2 are hypothetical control lanes to test for 9DNA contamination. 1 3 1000 bp 600 bp Based on the gel image, which of the following statements is correct? 1. The samples loaded in lanes 1 and 2 were not reverse transcribed prior to PCR. 2. Results from lane 2 suggest that 9DNA contamination is present. 3. The upper band in lane 2 represents 9DNA. 4. The fact that lane 1 is blank is indicative that Drug A doesn't lead to expression of Gene X. O A. 1, 2, and 3 O B. 1 and 3 O C. 2 and 4 O D. 4 only O E. All of 1, 2, 3 and 4 are correct