Using UV-Vis, the result of the absorption of coffee is shown in the graph. Pls. refer to the graph in answering the items below. 1.0- 0.8- 0.6- 0.4 - 0.2- 0.0 240 260 280 300 320 340 360 Wavelength/nm v Based on the data above, caffeine is not absorbed at A. 320 nm · v The absorbance of light with the value of 0.6 is seen at the spectrum of light with a wavelength of В. 260 nm - v the lambda max is seen at C. 260-290 nm Absorbance/a.u

Using UV-Vis, the result of the absorption of coffee is shown in the graph. Pls. refer to the graph in answering the items below. 1.0- 0.8- 0.6- 0.4 - 0.2- 0.0 240 260 280 300 320 340 360 Wavelength/nm v Based on the data above, caffeine is not absorbed at A. 320 nm · v The absorbance of light with the value of 0.6 is seen at the spectrum of light with a wavelength of В. 260 nm - v the lambda max is seen at C. 260-290 nm Absorbance/a.u

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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The concentration and absorbance data below were collected for compound X. A sample of compound X of unknown concentration has a corrected average absorbance of 0.5621 (three replicate measurements). Calculate the y-intercept? Concentration Absorbance (mg/mL) 0.422 10 0.510 15 0.621 0.299 0.222 0.319 O 0.553
A 0.1 g amine-containing compound is dissolved in water then diluted to 100 mL. To get the concentration of amine in this compound, you subject it to spectroscopic analysis. So, you get 1 mL of the previously diluted sample then dilute it again to 250 mL for measurement. Then, you fill 3/4 of a 1-cm cuvette with this diluted sample then you run an analysis using an AAS. The recorded absorbance is 0.545 at 410 nm. Solve for the molecular weight of the compound. The molar absorptivity is 1.23 x 104 cm-1 mol-1 L.
Based on the data, calculate the concentration of analyte in the ORIGINAL sample in ppm. Report to 1 decimal place Sample: A 10.00 mL aliquot of the unknown was placed in a 100.0 mL volumetric flask and made up to the mark will distilled water. The absorbance of this solution is 0.185. Sample + Spike: A 10.00 mL aliquot of the unknown and a 13.00 mL aliquot of the 46.00 ppm stock were both placed in the same 100.0 mL volumetric flask and diluted to the mark with water. The absorbance of this solution is 0.326. ( Please type answer note write by hend)
7. Fill the absorbance value below; Path length 0.4 0.6 0.8 1.0 0. 0.2 25 12.5 6.25 3.125 % T 100 50 Absorbance
Using data from Table 1 plot a graph similar to Figure 4 in the manual that you will label as “Figure A - Bromophenol Blue Stock Solution Absorbance Spectrum”. Remember all graphs need x-y-axis labels and titles. Plot the graph of order 2 polynomial as the best fit curve. The best-fit curve should show the equation of the line, it does not need to pass through the origin (0,0).
The calibration output from a breathalyzer test was plotted. If a sample was tested to have an absorbance of 0.200 and the legal limit was 0.1% ethanol, what is the % ethanol level of alcohol in the person? Enter the numerical value only in decimal form. Absorbance vs. % Ethanol in Final Prepared Solution Absorbance at 580 nm 0.350 0.300 0.250 0.200 0.150 0.100 0.050 0.000 0.00 0.05 0.10 0.15 % Ethanol in Final Prepared Solution 0.20 0.25
Sample (μg) Absorbances (520 nm) Concentration 0.0 0.621 0 2.5 0.599 2.5 5.0 0.556 5 10.0 0.469 10 15.0 0.418 15 20.0 0.291 20 Plot a graph in which y axis represents for absorbance and x-axis represents for concentration From graph, y=mx + c Calculate the vitamin C concentrations of samples by the equation with consideration of dilution factors.
The best fit curve line to an absorbance vs. concentration plot for standard solutions of a dye has a slope of 0.1815 mL/mg and intercept of 0.0477. A sample solution prepared of the same dye has an absorbance of 0.464 at the wavelength used to establish the Beer's law plot. What is the concentration of dye in the prepared solution? Select one: O a. 0.132 mg/mL b. 2.29 mg/mL O c. 0.464 mg/mL O d. 2.82 mg/mL e. 0.0365 mg/mL Next page Law Tutorial Part 2 Jump to... Exp #9 - Laboratory Practical► Worksheet étv A
2. Add the absorbance of your Part III solution to the graph. Llse the of best fit of your qraph to calcuate its line concentration. Concentraton vs Absorbance 1.6 con c: 0.003M 1.4 1.2 0.8 0.6 0.4 0.2 0.0005 0.001 0.0015 0.002 0.0025 0.003 0.0035 Concentration Co.003M) Concentration you have calculated in 2 is CCrO, of Ag CrO4. calculate your experimental ksp of Ag CrO4. = [Cr O4]? Use it to 2. %3D ksp of AgCr Oy = CAg+] [CrO4] Absorbance te Out
The data in the table are used to create a calibration curve for the determination of RNA from its absorbance at 260 nm. Use a spreadsheet and the least-squares method to determine the slope and y-intercept of the best straight line fit to the calibration curve. Do not subtract the blank reading when creating the calibration curve. m= RNA (µg) 0.00 10.29 20.58 30.87 41.16 mass= Absorbance at 260 nm 0.227 0.452 0.786 1.078 1.296 Hg-1 Using the slope and intercept of the calibration curve, determine the quantity of RNA in a sample that gives an absorbance of 0.523. µg
A student weighed out 0.150 g of protein powder and dissolved it in 100 mL of water (Solution 1). The student then diluted this solution by transferring 1 mL into a 25 mL flask and diluting with water (Solution 2). Finally, 1 mL of that solution was transferred to a test tube and combined with 4 mL Bradford reagent. The absorbance of the solution in the test tube was 0.11. Assuming that the best fit linear line of the standard curve was y = 0.04144 x + 0.01521 (μ g mL), calculate the percent protein by mass in the original protein powder.
Make a standard calibration curve wherein the x-axis is the total concentration of standard 1 to 6 and the y axis is their corresponding corrected absorbance ..(Note never include the absorbance reading of the unknown sample when making a standard curve) Absorbance Reading Test Tube / Standard Glucose Concentration (mg/mL) Initial Absorbance Corrected Absorbance Blank 0.00 0.045 0.000 Standard 1 1.00 0.102 0.057 Standard 2 2.00 0.236 0.191 Standard 3 4.00 0.697 0.652 Standard 4 6.00 1.104 1.059 Standard 5 8.00 1.385 1.340 Standard 6 10.0 1.915 1.870 Unknown 2.42 0.361 0.316 Data Set for Quantitative Analysis Tube No. ABSORBANCE TRIAL 1 TRIAL 2 TRIAL 3 TRIAL 4 TRIAL 5 TRIAL 6 1 0.041 0.044 0.046 0.046 0.046 0.046 2 0.102 0.103 0.103 0.102 0.102 0.102 3 0.236 0.235 0.240 0.235 0.237 0.235 4 0.703 0.698 0.696…