Using the Michaelis-Mentan graph pictured (graphing initial velocity and PNPP concentration), identify Vmax and Km as well as possible. Then, using the Lineweaver-Burk graph (graphing 1/Vo and 1 / PNPP concentration), label the reciprocals of Vmax and Km on the graph and use them to calculate Km and Vmax
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Using the Michaelis-Mentan graph pictured (graphing initial velocity and PNPP concentration), identify Vmax and Km as well as possible. Then, using the Lineweaver-Burk graph (graphing 1/Vo and 1 / PNPP concentration), label the reciprocals of Vmax and Km on the graph and use them to calculate Km and Vmax.
![[S]
[PNPP] μM
0
200
400
600
800
1000
Initial
Velocity
[PNP]
µM/min
0.0013
0.568
0.979
1.29
1.69
1.87
Initial Velocity ([PNP] µM/min)
2.0
1.5
1.0
0.5
0.0
0
0.568
250
0.979
500
1.29
[PNPP] (μM)
1.69
750
1.87
1000](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F73fa9197-042e-4d32-abe3-15bcfa7699ca%2F05296ddf-001a-43cb-b6d5-431a8f31a450%2Fgy76ckz_processed.png&w=3840&q=75)
![1/[S]
(1/μµM)
0.005
0.0025
0.00167
0.00125
0.001
1/Initial
Velocity
(1/ µM/min)
1.761
1.021
0.7752
0.5917
0.5348
1/Initial Velocity (1 / µM/min)
2.0
1.5
1.0
0.5
0.0
0.001
0.002
0.003
1/ [PNPP] (1/µM)
0.004
0.005](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F73fa9197-042e-4d32-abe3-15bcfa7699ca%2F05296ddf-001a-43cb-b6d5-431a8f31a450%2F257zpj_processed.png&w=3840&q=75)
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- After three minutes, the concentration of drug Zip in the red blood cells is 10 mmoles l-1. What is the average rate of entry of drug Zip into the red blood cells in units of moles min-1 red blood cell-1 during the first three minutes after placing the red blood cells into the bathing solution? Assume that each red blood cell occupies about 1 x 10-13 liters.The standard curve to determine the amount of betacyanin is shown below. You extracted a red pigment from a beet disc (the mass of a disc is 2 g) using 10 ml of 20% ethanol solution. You measured absorbance of the solution above the beet disc every minute for a total time of 20 minutes. The increase in absorbance was linear during a period of time from 1 min to 10 min. The absorbance at 10 min was 0.8. Calculate the amount of betacyanin extracted from 1g of a beet tissue per minute. Explain your calculations. You can use Excel or a calculator.in food microbiology, how do you compute for concentration (M) and absorbance (A) of peroxidase activity on hydrogen peroxide at different pH values? (wavelength used = 415nm). can you please explain the calculations thank you if given: Temperature = 75 C Molar absorptivity coefficient = 10.5 Path length (cm) = 2 I = transmitted light = 0.45