Under what pH conditions can a protein not bind to the beads in a column?     pH = -pKa     pH = pI     pH = 7     pH = pKa In size exclusion/gel filtration chromatography, the elution order is dependent upon     Molecular weight     Concentration     Overall Charge     Enzymatic Activity

Biochemistry
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Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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Chapter1: Biochemistry: An Evolving Science
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  1. Under what pH conditions can a protein not bind to the beads in a column?

       

    pH = -pKa

       

    pH = pI

       

    pH = 7

       

    pH = pKa

    1. In size exclusion/gel filtration chromatography, the elution order is dependent upon

         

      Molecular weight

         

      Concentration

         

      Overall Charge

         

      Enzymatic Activity

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