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- f. e. ? H ㅅㅅ Cu Li (H* workup) 1 EQ Br2 CH3CO2HThe concentration of purified OXA-M290 is tested with a BCA assay. Serial dilutions of a bovine serum albumin (BSA) stock solution are prepared, then pipetted into a 96-well plate; each dilution of the BSA standard is tested in triplicate. Then, bicinchoninic acid and Cu2+ ions are added to all of the wells of the plate. After incubating the plate for 1 hour, a microplate reader is used to measure the absorbance of all of the wells in the plate at 560 nm. This generates the following data: BSA conc. (μg/mL), Replicate 1 Absorbance, Replicate 2 Absorbance, Replicate 3 Absorbance 40, 1.360, 1.403, 1.481 20, 0.750, 0.745, 0.810 10, 0.380, 0.344, 0.398 5, 0.198, 0.160, 0.183 2.5, 0.090, 0.100, 0.085 1.25, 0.038, 0.043, 0.051 0.625, 0.024, 0.028, 0.019 Prepare a calibration curve using these data. You can use Excel, R, SPSS or an equivalent graphing software. In this graph, plot absorbance (y-axis) against the concentration of the protein standard (x-axis). Calculate and plot…(Please answer all questions) 5-33. What are some advantages of using column chromatography to purify reaction products in the microscalelaboratory? 5-34. Discuss the similarities and dissimilarities of TLC, paper, and column chromatography. 5-35. Discuss the similarities and dissimilarities of HPLC and gas chromatography. 5-36. (a) What are the main advantages of using flash chromatography?(b) How can TLC be used in connection with flash chromatography? 5-37. Using the information presented on the right, please identify and explainwhich spot has an Rf value of 0.5.
- PBL 1: An enzyme with a Km = 1 X 102 M was assayed to convert the substrate with initial co ncentration of 3 X 10$M. Find the time required to convert 70% of substrate when Vmax = 2.167 M/min. Km In So 1 - (So – S) t = Vmax S' Vmax S = So - xS,Give the advantages and disadvantages of sequential injection analyzers compared to traditional flow Injection analyzers.percentage purity of an ibuprofen sample was determined by a reversed phase HPLC method as follows: Y Der ● A calibration curve of peak area versus concentration (ug/mL) of pure ibuprofen was constructed The ibuprofen sample for analysis was prepared by taking 10.0204 g of powder and dissolving it in/1Lbf distilled water (solution A). 1 ml of the latter solution was further diluted to IL with distilled water (solution B). Solution B was analysed by HPLC analysis Results The equation for the best-fit line of the calibration curve is: y=26820x-1323.8. ● The average peak area for the ibuprofen sample (solution B) was 266796.2 Calculate the percentage purity of the ibuprofen powder.
- You have a treatment process in which a microbial population consumes a substrate (chemical A) according to Monod kinetics, with Hmax=2 d', Ks = 350 mg/L, and Y 0.15 g cells/g A. What biomass concentration (in mg/L) must be maintained to result in a substrate utilization rate of -1000 mg/L- hr for a concentration of A equal to 5000 mg/L? %3D %3!In quality management, it is very important that you have a good background knowledge in both descriptive and inferential statistics. Since you already took up Biostatistics and Epidemiology during your Second Year, answer BRIEFLY the following questions on basic statistics: 1. Compare and contrast the three most common measures of central tendency mean, median and mode. 2. Differentiate standard deviation from coefficient of variation. 3. What is T-test?2 (1) When performing chemical measurements, replicate experiments are often needed. This is to mitigate (Systematic or Random) error. (2) A CHEM 215 student performed 5 measurements of the wt% of SO4²- of a solid power sample. The results are 8.75%, 8.85%, 8.89%, 8.71%, 8.64%. The standard deviation is 0.10%. What is the confidence interval for this student's experiment at 95% confidence level. The t values for 95% confidence for degree of freedom from 1 to 5 are 12.71, 4.303, 3.182, 2.776, 2.571, respectively. Show your work!
- h) i) j) Ph + НО OSI(CH3)3 Br PPH3 1) "BuLi /THF/rt / 1.5 h H CH3 homo /rt / 1 h 2) -Si(CH3)3 OCH3 TiCl4 CH₂Cl₂/-78°C / 15 min PPh3/1₂ (1 eq) imidazole / THE 0°C-itAn active air sampler was used to trap polyaromatic hydrocarbons (PAHs) in the air above the sea area with massive oil spill. Air was pumped through an activated charcoal adsorbent material at a flow rate of 10 L per min for 12 hrs. The PAHs were then eluted out of the adsorbent material using 10 mL hexane. The eluate was analyzed by GC-MS. Anthracene was one of the PAHs detected and was found to be present at a concentration of 81.1 µg/mL. Determine the concentration of anthracene in the air above the oil spill. Report your answer in mg/m³.Br &. ONa DMF aprotic 2° 4 Swk or Sw2 ?