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Two enzymes are immobilized on the same flat, nonporous surface. For enzyme A, the substrate is S₁. For enzyme B, the substrate is S₂. The product of the first reaction is S₂ S₁ EA S₂ Ев P

Two enzymes are immobilized on the same flat, nonporous surface. For enzyme A, the substrate is S₁. For enzyme B, the substrate is S₂. The product of the first reaction is S₂ S₁ EA S₂ Ев P

Introduction to Chemical Engineering Thermodynamics
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
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Two enzymes are immobilized on the same flat, nonporous surface. For enzyme A, the substrate is S₁. For enzyme B, the substrate is S₂. The product of the first reaction is S₂ S₁ EA S₂ Ев P
Transcribed Image Text:Two enzymes are immobilized on the same flat, nonporous surface. For enzyme A, the substrate is S₁. For enzyme B, the substrate is S₂. The product of the first reaction is S₂ S₁ EA S₂ Ев P
a) Figure 1 depicts the rate of the first reaction on the surface as a function of local concentration of S₁. If the bulk concentration of S₁ is 100 mg/L, and the mass-transfer coefficient is 4 x 105 cm/s, what is the rate of consumption of S₁ for a 1 cm² surface? What is the surface concentration of S₁? or 4.0 3.0 x106 2.0 mg cm² -s 1.0 20 40 60 S, (mg/l) 80 d[S₂] d[P] dt dt 100 120 Figure 1. Reaction rate data for problem 6. Reaction rate dependence on substrate 1 for reaction catalyzed by EA. b) The rate of the second reaction follows: Vm S2 surface Km + S2 surface Here Km = 5 mg/L (or 5 x 10³ mg/cm³) and Vm" = 4 x 106 mg/cm²-s. The bulk concentration of S2 [S2,bulk] is maintained as 5 mg/L, and the mass-transfer coefficient is the same for S₁ and S₂. Calculate [S2,surface] and the rate of formation of P (assuming all stoichiometric coefficients are one)
Transcribed Image Text:a) Figure 1 depicts the rate of the first reaction on the surface as a function of local concentration of S₁. If the bulk concentration of S₁ is 100 mg/L, and the mass-transfer coefficient is 4 x 105 cm/s, what is the rate of consumption of S₁ for a 1 cm² surface? What is the surface concentration of S₁? or 4.0 3.0 x106 2.0 mg cm² -s 1.0 20 40 60 S, (mg/l) 80 d[S₂] d[P] dt dt 100 120 Figure 1. Reaction rate data for problem 6. Reaction rate dependence on substrate 1 for reaction catalyzed by EA. b) The rate of the second reaction follows: Vm S2 surface Km + S2 surface Here Km = 5 mg/L (or 5 x 10³ mg/cm³) and Vm" = 4 x 106 mg/cm²-s. The bulk concentration of S2 [S2,bulk] is maintained as 5 mg/L, and the mass-transfer coefficient is the same for S₁ and S₂. Calculate [S2,surface] and the rate of formation of P (assuming all stoichiometric coefficients are one)
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