Three (3) true positives (antigen is present and patient's sera reacted) and three (3) true negatives (antigen is not present and patient's sera did not react): Question 3 options: Is a tool that can be used to increase the probability that any alloantibodies identified is/are correct Is an AABB requirement in order to prove each alloantibody specificity Proves that the antibody procedure was performed correctly Gives a P value of 95%

Three (3) true positives (antigen is present and patient's sera reacted) and three (3) true negatives (antigen is not present and patient's sera did not react): Question 3 options: Is a tool that can be used to increase the probability that any alloantibodies identified is/are correct Is an AABB requirement in order to prove each alloantibody specificity Proves that the antibody procedure was performed correctly Gives a P value of 95%

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter4: Basic Immunology And Immunohematology

Section4.3: Tests For Rheumatoid Factors

Problem 2.1CS

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What is the minimum number of reactive antigen-positive and non-reactive antigen-negative panel cells that must be present in order to identify a red cell antibody with statistical confidence? Please select the single best answer Two Three Five Ten
You just received the properly labeled blood bank specimen on patient Aran Stark. You decide to collect some background information about her known historical antibodies before beginning the work-up knowing that she has a history of anti-E, anti-K, anti-Jk^a, anti-Fy^a, anti-M and anti-Le^a. Which antibody can be neutralized? Which antibody is destroyed with 0.2M DTT treatment? Which antibody reactivity is enhanced by acidification? Which of the antibodies that are typically IgG in nature are destroyed by enzymes? Which are enhanced by enzymes? Which of these antibodies have been known to cause hemolytic transfusion reaction? Which of these antibodies are known to react at room temperature? Which of these antibodies react best at 37C? * When you complete the work-up, you note that the anti-Jk^a antibody is no longer detectable. Can the patient receive red blood cells that contain the Jk^a antigen? Why or why not?
What is a screening test? Immunoassay Enzyme immunoassay (EAI) Enzyme-multiplied immunoassay technique (EMIT) Fluorescence polarization Radioimmunoassay (RIA) Chromatography Thin-layer chromatography (TLC) Gas chromatography (GC) Liquid chromatography (LC) (i.e, high performance liquid chromatography or HPLC) What is a confirmatory test? Hyphenated technique. Combination of two sophisticated technologies (I.e., Gas Chromatography - mass spectrometry or GC-MS) or other modern and acceptable techniques (l.e., LC-MS, GC-MS-MS, or LC-MS-MS).
If the highest dilution with a color change in an ELISA test is 1:64, the antibody titer is 640. true false
Can you please check and answer the questions that not answered, that is not graded https://www.studocu.com/en-us/ document/university-of-arkansas/microbiology/other/immunology-lab-worksheet-student/7960193/view Questions from ELISA Simulation Introduction Document (posted in Lab Module 8 folder): 1. What term is the word “antigen” derived from? 2. Where are antibodies found in the body? Plasma of the blood 3. In general, what is the cause of an autoimmune disease? 4. What kind of antibodies are present in patients with systemic lupus erythematosus (SLE)? 5. What does ELISA stand for? enzyme-linked immunosorbent assay 6. In general, what is an ELISA test used to detect? to measure the concentration of antibodies or antigens 7. In an indirect ELISA, which antibody is linked to the enzyme? (i.e the primary antibody from the blood serum or the secondary anti-human antibody) Questions from HHMI Virtual Immunology Lab Website Diagnosis…
Which of the following media is capable of detecting all clinically significant antibodies while avoiding all clinically insignificant antibodies? Question 1 options: Gel technology Tube technique with PEG No single media is capable of doing this Solid phase
Due to a clerical error, several blood samples at a blood bank were not labeled. The three list below were conducted on each sample to identify each blood type. Test 1 = Unknown sample mixed with “anti-A” serum (contains type A antibodies) Test 2 = Unknown sample mixed with “anti-B” serum (contains type B antibodies) Test 3 = Unknown sample mixed with “anti-Rh” serum (contains type Rh antibodies) (A) When unknown sample A was tested, agglutination (clumping) of red blood cells occurred in test 1, 2 and 3. The blood type of sample A is… (B) When unknown sample B was tested, no agglutination (clumping) of red blood cells occurred in any tests. The blood type of sample B is… (C) When unknown sample C was tested, agglutination (clumping) of red blood cells occurred in test 2 and 3, but not 1. The blood type of sample C is…
The technician decided to antigen type the patient to confirm predictions from the antibody panel. Antigen type results are given below. Given these results, which of the following statements is BEST supported? Rh Kell Duffy Kidd anti-D anti-C anti-E anti-c anti-e anti-K anti-Fya anti-Fyb anti-Jka anti-Jkb 3+ 0 3+ 3+ 0 0 0 3+ 2+ 2+ Question 7 options: A) K and Fya antibodies are likely because patient would not readily produce antibodies against their own cells. B) An anti-e antibody must be present due to the lack of antigen on the patient cells. C) Jka and Jkb antibodies are likely because an autoantibody is present. D) Rh antibodies are likely because the patient is Rh negative.
A technologist failed to notice that the centrifuge had not properly centrifuged the test tubes prepared for antibody identification. The time of centrifugation was 15 seconds instead of 30 seconds. What would be the potential error in the interpretation of this test? can please any one provide me ans?
Patient Testing Solution (Mr. Smith) Reaction Phenotype (Blood Type) Possible Genotypes for blood type (Clumping? Antibody-A Antibody-B | Antibody-Rh clumping no clumping clumping A+ AA or AO 1. a) Using a Punnett square determines one possibility of what the patient's (Mr. Smith) parent's blood types could be. For the Punnett square, ignore the Rh+ and Rh-. b) Could the parents be a possible donor? Explain why or why not? You may consider Rh+ and Rh- here. c)Which donor would you recommend, if any? Explain your choice using concepts and vocabulary from the course. You may use words, drawings or diagrams to clarify your choice. (Approximately 1 paragraph in length). 20 tv MacBook Pro & %23 $ % 2 3 4 7 E R Y P D F K C V 36 MOSISO comm nd
What are the important considerations that you have to remember in antibody screening? What are the important considerations that you have to remember in antibody identification What is the role of antibody screening in pretransfusion compatibility testing?
When a patient exhibits a four-fold or greater rise in antibody titer against a specific microbial antigen between acute-phase and convalescent-phase serum samples, this is considered to be a "seroconversion". Which of the following would be a correct interpretation of a seroconversion? None of the other four answers are a correct interpretation of a seroconversion These findings cannot be interpreted because antibodies aren't specific enough for antigens There is no evidence of current active infection because that would require that the antibody titer is decreasing rather than increasing These results show that the microbe itself has been detected in the patient's sample(s) The results show evidence of current or recent infection by the specific microbe because there has been an active immunologic response to its antigen

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	Is a tool that can be used to increase the probability that any alloantibodies identified is/are correct
	Is an AABB requirement in order to prove each alloantibody specificity
	Proves that the antibody procedure was performed correctly
	Gives a P value of 95%