This lab examines the relationship between the absorbance of light by a solution at 595 nm and the concentration of the Coomassie Blue dye-BSA protein complex in the solution. State whether the following descriptions of the lab experiment are valid or not, and explain why you say Yes or No: a. The experiment would be significantly more accurate if absorbance readings were recorded for a range of wavelengths, not just for 595 nm b. The experiment has limited accuracy because it does not account for the absorbance of light by the other components (components that are not the dye-protein complex, such as excess dye that is not bound to any protein) of the solution. c. The absorbance reading measures practically all the protein content in the solutions
Enzyme kinetics
In biochemistry, enzymes are proteins that act as biological catalysts. Catalysis is the addition of a catalyst to a chemical reaction to speed up the pace of the reaction. Catalysis can be categorized as either homogeneous or heterogeneous, depending on whether the catalysts are distributed in the same phase as that of the reactants. Enzymes are an essential part of the cell because, without them, many organic processes would slow down and thus will affect the processes that are important for cell survival and sustenance.
Regulation of Enzymes
A substance that acts as a catalyst to regulate the reaction rate in the living organism's metabolic pathways without itself getting altered is an enzyme. Most of the biological reactions and metabolic pathways in the living systems are carried out by enzymes. They are specific for their works and work in particular conditions. It maintains the best possible rate of reaction in the most stable state. The enzymes have distinct properties as they can proceed with the reaction in any direction, their particular binding sites, pH specificity, temperature specificity required in very few amounts.
This question is based on Bovine Serum Albumin
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