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- What mass of sodium glycolate (NaC2H3O3) should be added to 400.0 mL of 1.00 M glycolic acid to produce a buffer solution with a pH of 4.00? Ka = 1.47 x 10-4. Please indicate the full solutions.What would be the working stock concentration (in molarity) if you diluted 250 uL of a stock concentration of 10 uM in a total volume of 1 mL?Calculate the unknown concentration of the PROTEIN C with an absorbance value of A412 given the standard curve indicated in the table below. Write your final answer (NUMBER ONLY) in two decimal places rounded off. Protein concentration (µg/mL) 0 0.02 0.04 0.06 0.08 0.10 Your answer APIENT Absorbance 0.000 0.161 0.284 0.438 0.572 0.762
- How much of the enzyme proteinase k (solute) is required to make 250ml of a solution with a concentration of .01mg/ml? (Weight/volume ratio)Calculate pNF concentration in each cuvette using Beer-Lambert’s Law. ε = 18,000 M-1 cm-1; b = 2.00 cm Cuvette 1 pNF 1 mL Water 2 mL Absorbance 0.546Calculate the molarity (M) of a 65% (w/v) copper sulfate solution (m.w. = 160.0 g/mol).
- You are supplied with the following: NaCl (Mr = 58.443 g/mol) 250 mM Tris-Cl, pH 8 solution (1 Litre) EDTA, sodium salt (Mr = 380.2 g/mol) 13 % Sodium dodecyl sulfate solution Proteinase K solution (150 mg dissolved in 1 ml ddH2O) You need a digestion buffer consisting of the following: 23.5 mM NaCl 750 uM Tris-Cl, pH 8 38 mM EDTA, pH 8 1.2 % Sodium dodecyl sulfate 1.75 mg/ml proteinase K Calculate: How will you prepare 750 ml of the digestion buffer? Show all your steps and calculations and remember to explain how exactly you will make it up.What mass of sodium glycolate (NaC2H3O3) should be added to 400.0 mL of 1.00 M glycolic acid to produce a buffer solution with a pH of 4.00? Ka = 1.47 x 10-4Calculate the theoretical pH of a Tris buffer (pH 8.0) at 0 ºC. Assume that room temperature is 22 ºC