The total volume of the PCR sample is 50 ul. What volume of the PCR buffer is needed if it is supplied a The total volume of the PCR sample is 50 ul. What volume of the PCR buffer is needed if it is supplied as a 2x buffer? ul (use a whole number, no decimal point)
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- 2 ml 2 ml 2 ml 2 mL 2 mL 4 ml DEA buffer 2x 10 M substrate Based on the serial dilution scheme above, what is the substrate concentration in the last tube (each tube has 4 mL to start)? 2.3x 10-6 M 78x10-4M 6.3x 10-7 M 70x10-7M0.200 M 0.00200 M Test Tube No. [FESCN2*1std (M) Absorbance Fe(NO3)3 (mL) KSCN (mL) 17.98 1.97 0.815 5 (Standard) Edit Edit Edit NG 1 InfoIf an analytical method relies on a measurable property of an analyte that can be correlated with the amount of the said analyte, what type of analytical method is it? Oxidative methods Instrumental methods Blank Correction methods Classical methods
- 26. You are provided with a stock solution - 2 mg/ml BSA, calculate and fill in the blanks in the table below for preparation of the solutions (final volume is 1 mL); and draw a standard curve. Final concentration of BSA BSA stock (uL) Buffer (ul) Absorbance (mg/ml) (540 nm) in protein assay 2 1.5 1 1.1 0.75 0.7 0.5 0.3 0.25 0.1This is a Biochemistry lab question. For the lab we are spectrophotometrically determining the pka of PNP based on different pHs. I have a sigmoid graph of the absborances of PNP at different pHs at 400 nm. I have pH on the x-axis and absorbance on the y-axis. How do a calculate the pka based off of this graph?Tube #: 2 3 4 mL 0.0120 M Pb(NO,), 5.00 5.00 5.00 5.00 2.00 3.00 4.00 5.00 mL 0.0300 M KI mL 0.20 M KNO, 3.00 2.00 1.00 0.00 Total vol. (mL) 10.00 10.00 10.00 10.00 Absorbance of soln. 0.381 0.420 0.470 0.542 [I] at equilibrium (M)
- Data Collection Kinetic Data Collection Time, t (s) Volume of added base (mL) First addition 40 0.50 Second addition 101 1.00 Third addition 160 1.52 Fourth addition 224 1.99 Fifth addition 278 2.49 Sixth addition 334 3.01 Seventh additlon 389 3.48 Calculations Concentration of NaOH stock solution: 0.01 M Concentration of t-BuCl stock solution: 0.2 M Volume of t-BuCl solution: 10 mL stock stock solution added to 25 mL acetone Table view List view Kinetic Data Volume of Moles of Amount of t-Bucl (mol) [-Buc] In[-BuC Time, t (s) added base added base (mL) (mol) First addition 40 0.50 Second additlon 101 1.00 Third addition 160 1.52 Fourth addition 224 1.99 Flfth additlon 278 2.49 Sixth additlon 334 3.01 Seventh addition 389 3.48 Use the data in the table above to plot In[t-BuC]]; vs time (s). Include labelled axis, a title, and a linear trendline. Upload your plot here.calculate the [FESCN2+] using volumes of stock solutions. Presume that all of the SCN- ions react. Next, record the light absorbance values of each standard solution. Volume Volume Volume [FESCN2+] Absorbance Standard Fe(NO3)3 SCN- H2O sample (mL) (mL) (mL) 1 2.50 2.00 20.50 0.1811 2 2.50 1.50 21.00 0.3219 2.50 1.00 21.50 0.4981 4 2.50 0.50 22.00 0.6328 Stock [Fe(NO3)3] = 0.200 M, Stock [SCN-] = 0.0020 M. %3DThe protein content of the unknown samples were determined using BSA as standard. The absorbance values of the standard solutions and samples are as follows. BSA concentration (mg/mL) Absorbance 0.000 20 0.110 40 0.192 60 0.248 80 0.325 100 0.400 120 0.460 a. What is the equation of the line? b. Determine the protein concentration of samples in ug/mL. Sample dilutions 1 in 14 A540 0.150 1 in 5 1 in 8 0.380 0.235
- What is two types of ionization method in GC MSD?A solution of potassium permanganate of unknown concentration was analyzed against a standard of various concentrations. Table 1. Data for Standard Calibration Curve Absorbance at 525 nm Potassium permanganate Standard (mol/Liter) 0.00040 0.104 0.00080 0.258 0.00120 0.453 0.00160 0.682 0.00200 0.894 What is the concentration of potassium permanganate if its absorbance is 0.465? none of the above 0.00137 M KMnO4 O 0.00155 M KMnO4 0.00117 M KMnO4 0.00153M KMnO4If Jotaro Kujo measured the amount of current that passes through a sample to measure the amount of analyte in the sample, what kind of method did he use? Volumetric method Spectroscopic method Gravimetric method Electroanalytical method