The stock erythrosine B solution is 8.5 × 10-5 M. Calculate the volume of this solution that you must pipet into a 50-mL volumetric flask, which, when diluted to mark with deionized water, will give a final concentration of 1.7 × 10-5 M. Show your calculation setup.
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The stock erythrosine B solution is 8.5 × 10-5 M. Calculate the volume of this solution that you must pipet into a 50-mL volumetric flask, which, when diluted to mark with deionized water, will give a final concentration of 1.7 × 10-5 M. Show your calculation setup.
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- Write your complete solutions on yellow paper, round your answers to the appropriate number of significant figures, and box the final answer. The deadline for submission is at the end of the class. Note: You do not need to write your solutions for the items that can be derived from your calculators. 1. Three students (Saerom, Hayoung, and Jiwon) are asked to determine the ppm Ca in a drinking water sample by titration with EDTA. Each student performed five trials for the sample. The results in ppm Ca are the following: Saerom Hayoung Jiwon a. Get the mean, SD, and % RSD of each student's results. b. If the true value is 35 ppm, calculate the relative error of each student's results. c. Comment on the precision and accuracy of each student's results. The maximum % RSD and the relative error set by the lab is 5.0%. d. Use the following table to summarize your answers. Mean SD %RSD Relative Error Comment 31, 32, 33, 33, 32 35, 34, 34, 35, 34 35, 38, 33, 35, 37 Saerom Mean SD 95% CI…1. What is the concentration of the solution prepared by diluting 200 µl of kanamycin (25 mg/ml) to a final volume of 2 ml? 2. How many microliters of a 0.1 mM Mg(OH)2 solution would be needed to provide 58.32 ng of Mg(OH)2 for a chemical reaction? [Mwt Mg(OH)2 = 58.32 g/mole] 3. The final amount of each primer required in a PCR reaction is 0.025 nanomol. If the total volume of the PCR reaction is equal to 100 µl and the stock concentration of each primer is equal to 10 µM. Calculate the volume of stock primer that needs to be added in order to ensure a primer amount of 0.025 nanomol.Congratulations, you have just been hired at a local laboratory. Your first task is to prepare 10.0 mL of a 1.50 x 10-6 M solution of a small protein (FW: 3600 g/mole). Available to you are an electronic balance that is accurate to the nearest 0.001 g, a 1.00-mL pipette, and 10-mL volumetric flasks. Describe how to prepare the desired solution. (Note that the amount of protein is so small that you will need to make a more concentrated stock solution and dilute it.)
- You obtain an Unknown Sample from the Stockroom. You begin testing the solution through the steps outlined in the flowchart of the experiment’s PDF file. You first add HCl and centrifuge your mixture. You observe the formation of a white precipitate in the bottom of the test tube. After pouring off the supernatant liquid, you add hot water to the white precipitate. Upon addition of the hot water, you have some white precipitate still at the bottom of the test tube, and the supernatant liquid is poured off. To this liquid, potassium chromate (K2CrO4) is added. Upon this addition of potassium chromate, you observe no formation of precipitate. Which of the following is a valid conclusion to draw at this point? (Choose one.) Select one: 1. Hg22+ is definitely present. 2. Pb2+ is NOT present. 3. Ag+ is definitely present. 4. Pb2+ is definitely present.You're at the same pharmaceutical company, and they also make magnesium pills. You weigh out 5 tablets (3.2834 grams). And crush them with a mortar and pestle. You take a subsample of 384 mg and dissolve it in acid, making it to a final volume of 250mL. • You then take 50 mL and dilute it to 250mL volumetrically. • You further dilute the sample by taking 15 ml and diluting it to 250mL. • You run this on a varian AA with your standards and get the following response: Response (ABS) 0.546 1.134 2.135 1.634 ● ● Solution 1.0 ppm Mg 2.0 ppm Mg 4.0 ppm Mg Sample Calculate the amount of Mg in one pill. Line of best fit y = 0.5255x+0.0455Weigh out accurately 9.99 g of copper(II) sulfate pentahydrate (CuSO4·5H2O) by taring using a clean and dry empty 250-ml beaker. 2. Add tap water to the beaker to reach the 50-mL mark on the side of the beaker. 3. Dissolve all the crystals of CuSO4·5H2O in the water using a clean glass rod for stirring. 4. Then using a glass funnel transfer all the blue solution to a 100-mL volumetric flask. Rinse the beaker with about 10 mL of water and transfer the solution to the volumetric flask. Repeat the rinsing of the beaker with another 10 mL of water and transfer the solution to the volumetric flask. This is called quantitative transfer of the solution. 5. Then add enough water to reach the calibration mark of the volumetric flask. (You will need to use a dropper to add the last few drops of water to ensure the meniscus is on the calibration mark). Close the volumetric flask with a stopper and mix the solution well (so that it is homogeneous). 6. You have now prepared a standard solution (or…
- 4. Determine the molarity of a solution prepared by dissolving 155.6 g of citric acid, C3H5O(COOH)3, in water and then diluting the resulting solution to 4500.0 mL. please show workFive white, 500-mg uncoated ascorbic acid (AA) tablets with an average weight of 0.6152-g were pulverized in a mortar. A sample of the powdered ascorbic acid weighing 0.4700-g was placed in an iodine flask and was dissolved in 50-mL H2SO4 then 5-g of KBr was added to the resulting solution. The solution was titrated with 47.81-mL of 0.09640 N STD. KBrO3 to reach a faint yellow endpoint then 3-g KI and 5-mL Starch TS. The blue color solution is then titrated with 2.73-mL of 0.09123 N STD. Na2S2O3 to reach the disappearance of the blue iodostarch complex. MW: KBrO3 = 167.0 ; KIO3 = 214.0 ; Na2S2O3 = 158.11 ; C6H8O6 = 176.12 Compute the milligrams of pure AA per tablet from the assay. 293.3 mg 502.5 mg None of the choices 383.9 mgWhy is water not used as a solvent in extraction of nuts
- You obtain an Unknown Sample from the Stockroom. You begin testing the solution through the steps outlined in the flowchart of the experiment’s PDF file. You first add HCl and centrifuge your mixture. You observe the formation of a white precipitate in the bottom of the test tube. After pouring off the supernatant liquid, you add hot water to the white precipitate. Addition of the hot water dissolves some of the precipitate, but some white precipitate still remains on the bottom of the test tube. You pour off the supernatant liquid, and add ammonia (NH3) to the remaining precipitate. You now observe the formation of a gray-black precipitate. Which of the following is a valid conclusion to draw at this point? Select one: Hg22+ is definitely present. Pb2+ is definitely present. Ag+ could be present, or Hg22+ could be present, or BOTH could be present. Ag+ is definitely presentYou obtain an Unknown Sample from the Stockroom. You begin testing the solution through the steps outlined in the flowchart of the experiment’s PDF file. You first add HCl and centrifuge your mixture. You observe the formation of a white precipitate in the bottom of the test tube. After pouring off the supernatant liquid, you add hot water to the white precipitate. Addition of the hot water dissolves some of the precipitate, but some white precipitate still remains on the bottom of the test tube. Which of the following is a valid conclusion to draw at this point? Pb2+ is definitely present. Hg22+ is definitely present. Ag+ could be present, or Hg22+ could be present, or BOTH could be present. Ag+ is definitely presentA sample containing chlorophenol, C6H4ClOH, was analysed by gravimetric analysis. A 0.783-g of this sample goes through a chemical process allowing the chlorine in the chlorophenol to precipitate as silver chloride, weighing 0.271 g. Calculate the % (wt/wt) of the chlorophenol in the sample, assuming that chlorophenol is the only source of chlorine in the sample. Provide your answer to two decimal places and without units. Avoid using scientific notation.