The Quikchange cycling parameters are listed below: 1.95 degrees, 2 min 2. 95 degrees, 20 sec 3. 60 degrees, 10 sec 4. 68 degrees, 2 min 5. Repeat steps 2-4 18 times 6. 4 degrees, indefinitely Match each step above to the correct function(s) listed below. Write your selection(s) for each step. You may have more than one answer for each. 1.initial denaturation 2. dNTP pairing 3. renaturation 4. Primer annealing 5.new DNA extension
The Quikchange cycling parameters are listed below: 1.95 degrees, 2 min 2. 95 degrees, 20 sec 3. 60 degrees, 10 sec 4. 68 degrees, 2 min 5. Repeat steps 2-4 18 times 6. 4 degrees, indefinitely Match each step above to the correct function(s) listed below. Write your selection(s) for each step. You may have more than one answer for each. 1.initial denaturation 2. dNTP pairing 3. renaturation 4. Primer annealing 5.new DNA extension
Chapter16: Adult And Pediatric Dosages Based On Body Surface Area
Section: Chapter Questions
Problem 12SST
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The Quikchange cycling parameters are listed below:
1.95 degrees, 2 min
2. 95 degrees, 20 sec
3. 60 degrees, 10 sec
4. 68 degrees, 2 min
5. Repeat steps 2-4 18 times
6. 4 degrees, indefinitely
Match each step above to the correct function(s) listed below. Write your selection(s) for each step. You may have more than one answer for each.
1.initial denaturation
2. dNTP pairing
3. renaturation
4. Primer annealing
5.new DNA extension
6. polymerase unwinding
7. refrigeration
8.secondary denaturation
9. degradation of excess DNA
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