The Quikchange cycling parameters are listed below: 1.95 degrees, 2 min  2. 95 degrees, 20 sec  3. 60 degrees, 10 sec 4. 68 degrees, 2 min  5. Repeat steps 2-4 18 times  6. 4 degrees, indefinitely  Match each step above to the correct function(s) listed below. Write your selection(s) for each step. You may have more than one answer for each.  1.initial denaturation  2. dNTP pairing  3. renaturation  4. Primer annealing  5.new DNA extension

Curren'S Math For Meds: Dosages & Sol
11th Edition
ISBN:9781305143531
Author:CURREN
Publisher:CURREN
Chapter16: Adult And Pediatric Dosages Based On Body Surface Area
Section: Chapter Questions
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The Quikchange cycling parameters are listed below:

1.95 degrees, 2 min 

2. 95 degrees, 20 sec 

3. 60 degrees, 10 sec

4. 68 degrees, 2 min 

5. Repeat steps 2-4 18 times 

6. 4 degrees, indefinitely 

Match each step above to the correct function(s) listed below. Write your selection(s) for each step. You may have more than one answer for each. 

1.initial denaturation 

2. dNTP pairing 

3. renaturation 

4. Primer annealing 

5.new DNA extension 

6. polymerase unwinding 

7. refrigeration 

8.secondary denaturation 

9. degradation of excess DNA

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