The potentiality of replication of deoxyribose nucleic acid depends on? Covalent bonds between bases Electrovalent bonds between bases and phosphate groups High energy bonds between phosphate groups Hydrogen bonds between bases Hydrogen bonds between base and sugar groups
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The potentiality of replication of deoxyribose
Covalent bonds between bases
Electrovalent bonds between bases and phosphate groups
High energy bonds between phosphate groups
Hydrogen bonds between bases
Hydrogen bonds between base and sugar groups
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- What is the complementary strand to the following nucleic acid sequence? 5’ ATCAGCCG 3’ 5’ TAGTCGGC 3’ 3’ TAGTCGGC 5’ 5’ GCAGTAAT 3’ 3’ GCAGTAAT 3’Match the proteins with their function in DNA replication. unwinds DNA at replication fork forms covalent phosphodiester bonds to join DNA fragments remove primer and replace the RNA with DNA, also has 3'-> 5'exonuclease activity for proofreading synthesizes DNA by adding to the 3' end of the growing strand of DNA adds RNA primer uses an RNA template to facilitate extension of the telomeres in eukaryotes helicase DNA ligase DNA polymerase II introduces a double-stranded break ahead of the replication fork and swivels the cleaved ends around the central axis to relieve the stress of helix unwinding DNA polymerase III DNA primase telomerase DNA Gyrase (topoisomerase)Given the active site diagram below, please identify the residue participating in a charge-charge interation. HO OH HN OH 5 ΝΗ *HN ΝΗ OH O Zn²+ 2 4 5 3 1 2 NH 3
- How many hydrogen bonds exist between this DNA strand and its complementary strand? 5'-GCGACCG-3"DNA Replication: 1. Write in the new (complimentary) strands for each of the two halves of the DNA molecule below to show what the two semi-conserved strands would look like. A G T A C C G G G C A A A C T G C A T T G T G I I I I I I I I I I I I I I I I I I I I I I I I I I T C A T G G C C C G T T T G A C G T A A C A C old strand A G T A C C G G G C A A A C T G C A T T G T G new strand: new strand: old strand T C A T G G C C C G T T T G A C G T A A C A C Gene Expression: 2. Use your DNA strand to construct a part of a messenger RNA molecule. DNA A G T A C C G G G C A A A C T G C A T T G T G mRNA:Match the proteins with their function in DNA replication. unwinds DNA at replication fork forms covalent phosphodiester bonds to join DNA fragments remove primer and replace the RNA with DNA, also has 3'-> 5'exonuclease activity for proofreading synthesizes DNA by adding to the 3' end of the growing strand of DNA adds RNA primer uses an RNA template to facilitate extension of the telomeres in eukaryotes introduces a double-stranded break ahead of the replication fork and swivels the cleaved ends around the central axis to relieve the stress of helix unwinding helicase [Choose ] ✓ helicase telomerase DNA polymerase II DNA polymerase I DNA polymerase III DNA primase DNA ligase D DNA Gyrase (topoiso- merase) exonuclease
- During bacterial replication, concurrent synthesis is assisted by the specific action of gyrase O DNA clamp O helicase single stranded binding proteinsConsider normal B-form DNA. It forms a regular antiparallel double-helical structure with Watson-Crick base-pairing mediated through hydrogen bonding. The base pairs all stack upon one another, with 3.4 Å spacing between them. DNA strands having a complementary sequence will spontaneously form a double-helix in an aqueous solution. In terms of energy, what primarily drives helix formation? O Positive Entropy from base stacking van der Waals interactions O Hoogsteen interactions Positive Enthalpy from Hydrogen Bonding between GC and AT pairs Negative Enthalpy from Hydrogen Bonding between GC and AT pairs O Negative Entropy from base stackingIf a sequence of one strand of DNA is 5'-TGACTATC-3', what is the complementary strand? 5'-ACTGATAG-3' 3'-ACTGATAG-5' 3'-ACTGATAG-3' 3'-TGACTATC-5' 5'-TGACTATC-3'
- Origin of replication [ Choose ] [Choose] Codon Complementary pairs with cytosine in a DNA molecule The site of protein synthesis Ribozyme This molecule can begin the synthesis of a polymer of nucleotides without a primer A short peice of lagging stand DNA Primase A reflection of redundancy in the genetic code RNA which exhibits enzymatic properties Okazaki Fragment The process of making RNA from DNA Helicase DNA polymerase Transcription Enzyme which lays down a segment of RNA on replicating DNA so that DNA nucleotides can polymerize A sequence of three nucletotides which codes for an amino acid Ribosome Point Mutation Chromosome Wobble Position The point on a stand of DNA where replication begins Mitochondria RNA polymerase [ Choose ] GuanineDuring DNA replication, the function of RNA primers is to Group of answer choices serve as a binding site for DNA ligase separate the two strands of the double helix to open replication "bubbles" serve as starting points for DNA strand elongation by DNA polymerase in the 3' - 5' direction prevent new-separated strands of DNA from rejoining serve as starting points for DNA strand elongation in the 5' - 3' direction by DNA polymeraseYou turn up the heat to 95° Which is the most likely outcome? Explain your answer. Both duplexes are now single-stranded oligonucleotides Both duplexes are now individual dNTPs Both duplexes remain duplexes The nitrogenous bases are released from the 1’ C of deoxyribose
