The methodology of choice for measuring therapeutic drug levels is Question 4 options: immunoassay chromatography atomic absorption spectrophotometry spectrophotometry
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The methodology of choice for measuring therapeutic drug levels is
Question 4 options:
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immunoassay |
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|
chromatography |
|
|
atomic absorption spectrophotometry |
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spectrophotometry |
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- What are the 5 important characteristics of spectrophotometric methodsA solution of a dye was analyzed by spectrophotometry, and the following calibration data were collected using a 1 cm cuvette. *graph in attachemnt* Using the calibration data above for the dye solution, what is the dye concentration in a solution with an absorbance (A) = 0.52 if it was measured in a 1 cm cuvette? Group of answer choices 3.0 x 10-6 M 6.0 x 104 M 2.0 x 10-6 M Not enough information is provided.Quantitative Analysis by External Standard Method Calcium in a juice sample is determined by atomic absorption spectrophotometry. A stock solution of calcium is prepared by dissolving 1.834 g of CaCl, 2H20 in water and diluting to 1000.0 mL. A substock calcium solution was prepared by transferring 10.00 mL of the stock to a 100.00 mL volumetric flask and diluting to the mark. Three standard solutions of calcium were prepared by transferring 2.500 mL. 5.00 mL and 10.00 mL of substock to 50.00 mL volumetric flasks and diluting to the mark. The sample is prepared by transferring 1.000 mL of fruit juice from the original bottle to a 25.00 mL flask and diluting to the mark. Strontium chloride is added to all solutions before dilution to avoid phosphate interference. Instrumental response of the standards is measured against the blank, and the following readings are recorded: 9.10, 18.6 and 37.0. The sampie reading is 28.1. Using the multipoint method, find the concentration of calcium in…
- For the determination of Fe3 +, 25 mL of sample taken from sea water is diluted to 100 mL and a quantity of the obtained solution is taken and Fe3 + is determined by atomic absorption spectrophotometer. The absorbance values of the standard and sample solutions are below, and calculate the Fe3 + concentration in sea water in mg / L by drawing the relevant graph.Solution Absorbance1 mg / L Fe3 + 0.04095 mg / L Fe3 + 0.205110 mg / L Fe3 + 0.411615 mg / L Fe3 + 0.620820 mg / L Fe3 + 0.8222Sample Solution 0.5155The validation process comprises four main steps that are : hardware validation, software validation, method validation and system suitability. To evaluate a spectrophotometer’s accuracy by measuring absorbance, a solution of 60.06 mg/L K2Cr2O7 in 0.0050M H2SO4 was carefully prepared together with a 0.0050 M H2SO4 as a reagent blank. If the spectrometer’s signal is properly calibrated, it has been reported that this solution should provide an absorbance of 0.640 +- 0.010 at wavelength 350 nm. Which one of the validations mentioned above had been involved in this process? Explain your choiceIn the process of performing a spectrophotometric determination of Fe, an analyst prepares a calibration curve using a single-beam spectrometer, such as a Spec-20. After preparing the calibration curve, the analyst drops the cuvette used for the method blank and the standards. The analyst acquires a new cuvette, measures the absorbance of the sample, and determines the %w/w Fe in the sample. Will the change in cuvette lead to a determinate error in the analysis? Explain.
- Describe how to use a spectrophotometerA) Given the following set of data, plot the chromatogram (absorbance versus volume of eluate). Vol of eluate (mL) Abs (560 nm) 0.5 0.182 1 0.108 1.5 0.042 2 0.02 2.5 0.015 3 0.083 3.5 0.052 4 0.03 4.5 0.011 0.004 5.5 6. 0.001 6.5 0.001The students conducted the assay for LDH activity using the two serum samples. Each cuvette (path length 1 cm) contained 3ml of a suitable assay buffer (including pyruvate as substrate). 20 microlitres of either serum was added to the cuvette and the absorbance values immediately recorded at the optimum wavelength for a period of 5 minutes (absorbance readings taken every 30 seconds). Protein concentration of serum sample (mg/ml) Change in absorbance at optimum wavelength per minute Control serum (C) 8 -0.04 Diseased serum (D) 7.8 -0.6 1c. Using the molar absorption coefficient of NADH as 6220 M-1 cm-1, and by application of the Beer-Lambert law, estimate the enzyme activity in the two samples (C and D). Express activity as moles per second. 1d. Estimate the specific activity of the two samples (moles per second per microgram).
- what is spectrophotometry? how is it related to Beer's Law?An unknown sample of Cu2+ gave an absorbance of 0.475 in an atomic absorption analysis. Then 5.00 mL of solution containing 75.0 ppm (= µg/mL) Cu2+ was mixed with 45.0 mL of unknown, and the mixture was diluted to 500.0 mL in a volumetric flask. a) Write an expression for the final concentration after dilution. b) Calculate the final concentration of the added standard. c) The absorbance of the new solution was 0.987. Find the initial concentration of Cu2+ in the unknown sampleExplain why is FAAS a good method of quantifying Ca in milk?
