The experiment file says "Fill a cuvette with water and blank the spectrophotometer." What are we doing when we do this? Why is this step important
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The experiment file says "Fill a cuvette with water and blank the spectrophotometer." What are we doing when we do this? Why is this step important
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- What are the significance of UV/Vis spectrophotometer in the experiment complexation.What is the reason for the decrease in the ABS value over time when measuring in the spectrophotometer?The following image is a scheme for serial dilutions prepared for spectrophotometric analysis. If the stock solution concentration is 0.05 % (v/v) can you calculate the other tube’s concentrations in % v/v? I've used this with direct dilutions, how would I use this on serial dilutions?
- You may want to use this resource for this problem. If you do, submit the output along with your solution.You have been given a confocal microscope equipped with the following lasers, excitation filters, andemission filters:Laser Emission filter355 nm 410-470 nm405 nm 470-500 nm488 nm 500-550 nm532 nm 570-610 nm561 nm 610-650 nm640 nm 660-700 nm808 nm 720-780 nmYour task is to design an experiment to visualize the following:1. Nuclei2. A fluorescent protein in the cytosol3. A cell membrane marker antibody conjugated with a fluorophore4. Actin filaments5. LysosomesYou may choose from the following fluorophores for each of the five channels:Nuclei Fluorescent protein Membrane marker Actin marker Lysosome trackerDAPI GFP FITC AF488 Phalloidin LysoTracker RedHoechst 33342 YFP WGA-TRITC AF568 Phalloidin LysoTracker DeepRedSYTO Deep Red RFP Cy7 AF594 Phalloidin LysoTracker Blue Part 3.1Choose appropriate fluorophores for each of the subcellular structures to be imaged, taking into…What is Fluorescence Reflectance Imaging (RFI)? ExplainHow can you tell if the current or the gel electrophoresis is already running and what are the common mistakes or problems encountered in gel electrophoresis analysis?
- Is it possible to determine Km and Vmax without using excel? Because we are not allowed to use computer during exam, so I dont understand how its done without using excel?Here are the materials and method for the basic spectrophotometer experiment Materials:1. Paper template2. Scissor3. Light source (i.e. torchlight, portable light)4. Paperboard (black colour)5. Unused compact disc (CD)6. Camera detector (i.e. webcam, laptop, mobile phone) Methods: 1. Make a foldable paper spectrophotometer using the paper template and followthe instruction as in https://publiclab.org/sites/default/files/8.5x11minispec3.8.pdf2. Then, mounted the paper spectrophotometer to the camera detector.3. Download any freely available spectral analyzer.4. Test and observe the wavelength with and without the presence of a light source.5. Finally, observe the wavelength with a different transparent colour sheets (you canchoose any colour). Troubleshooting/ problem(s) encountered for basic spectrophotometer experiment.The field of view (FOV) is the entire circular image we see when looking into the eyepiece. The diameter of the FOV gets smaller as we increase magnification. It can be measured by using a stage micrometer like a ruler, measuring from edge to edge. Notice that the stage micrometer is 1000 microns (µm) in length, and the field of view under the lowest magnification is 5000 µm. Describe how we measure it?
- Imagine you will use a centrifuge in the laboratory indicating rpm and your protocol requires 3500xg, what would your setting be in a given centrifuge with a radius of 10cm? Provide computationsIn spectrophotometry, what is the role of the green background in the set-up? What does it represent in the actual spectrophotometer?WHAT ARE THE Steps in spectrophotometer?