The diagram below shows that of a plasmid containing genes that confers erythromycin (Erm®) and ampicillin (Amp®) resistance to bacteria that expresses these genes. On the outer portion of the diagram are the labels of some of the different restriction sites available for the insertion of foreign DNA. Part Scal Erm" plas02 EcoRI Pu (4.361bp) EcoR V BamHI Amp Sall651 The DNA segment that codes for the protein insulin was inserted into the plasmid using the restriction site BamH I. Which of the following is true if bacteria were able to successfully incorporate a plasmid with the insulin gene insert? The bacteria will not have any resistance to ampicillin or erythromycin. O The bacteria will exhibit resistance to both ampicillin and erythromycin. The bacteria will exhibit resistance to ampicillin but not to erythromycin. O The bacteria will exhibit resistance to erythromycin but not to ampicillin. O The bacteria will randomly exhibit resistance to either ampicillin or erythromycin, but not to both.

Q: In several experiments, you find these orders of genes getting transferred in from the chromosome…

A: F plasmid is a type of plasmid which allows the transfer of genes from one bacteria to the other…

Q: In the following "gene library" cloning experiment. Digested genomic DNA AmpR gene TCR gene TCR is…

A: Please follow step 2 for detailed explanation.

Q: Which statement is NOT the function of restriction enzyme?

A: Answer: It ligates the desired to gene to vector’s plasmid.

Q: The plasmid PSU922 is a circular DNA containing 25000 base pairs. The B-gal gene codes for the…

A: Restriction endonucleases, often known as restriction enzymes, are found in all prokaryotes. The…

Q: A researcher has a plasmid vector that has been cut with a restriction enzyme that cuts after the…

A: Restriction enzymes are also called "restriction endonucleases". They are a group of enzymes that…

Q: etion enzyme Sall cuts the sequence GTCGAC to leave a five base, 5' overhang. The restriction enzyme…

A: A restriction enzyme is classified under the category of a protein. These are isolated from bacteria…

Q: The drawing below depicts a restriction map of a segment of a human chromosome. The cut sites shown…

A: Since we only answer up to 3 sub-parts, we’ll answer the first 3. If you need help with other sub…

Q: You have a 5.3 kb PstI fragment cloned into the PstI site of the vector PUC19, which is 2.7 kb in…

A: In recombinant DNA technology, cloning is a technique that is used to generate identical copies of…

Q: Give 1 example of diseases arising from each of the following mutations and explain each. Point…

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A: Plasmids are used in genetic engineering to increase the number of copies of a gene. A plasmid is a…

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A: Recombinant DNA technology is helpful for different gene related experiments.

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A: Introduction :- Recombinant DNA technology ( also known as genetic engineering) involves the use of…

Q: arac PKAN-R 5,512 bp PARA 4,872 bp BamH PBAD Bam 377 bp kank ampR PBAD-rfp 807 bp Hindill Hindli

A: Answer :  The ristricton enzmye used to make cut in the plasmid.  Both the plasmid should be cut…

Q: Most bacteria which are used in molecular biology are ampicillin sensitive and recA. Plasmid usually…

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Q: Hindl EcoRI ECORV BamHI 373 Salt Pstl. tet amp Plasmid 1000 DBR322 4361 be ori Ndel you were going…

A: Restriction enzymes or restriction endonucleases/exonucleases are enzymes capable of recognizing a…

Q: A eukaryotic gene is added to a mixture containing a restriction enzyme EcoRI and a bacterial…

A: Which media allow growth of bacteria that did not take up any plasmid?

Q: Hey, I need help with this please: Plasmid pRIT450 is 7.0 kb in length and has single PstI, EcoRI,…

A: Given: A Plasmid pRIT450 of  7.0 kb in length. It has single PstI, EcoRI, and BamHI sites. The…

Q: A. Use this data to generate a circular map of the plasmid. Pst I: 10 kb Bam HI: 10 kb Sal I:…

A: Restriction endonucleases are enzymes that cut DNA at specific sites. Restriction endonucleases…

Q: A eukaryotic gene is added to a mixture containing a restriction enzyme EcoRI and a bacterial…

A: The restriction endonuclease is the enzymes that help in the deduction of the DNA from a specific…

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A:

Q: An E. coli Hfr (high frequency recombination) strain is defined as an E. coli strain that Select…

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Q: You are studying a new plasmid, and you digest the plasmid with three restriction enzymes: Eco RI…

A: DNA Plasmid -- A Plasmid is a small extra chromosomal DNA molecule which is physically separated…

Q: pls help very urgent, no explaantion required, pls help asap

A: In molecular biology, a restriction digest is a technique for preparing DNA for analysis or further…

Q: Make a drawing of the double recombinant plasmid containing all four fragments. Label fragment…

A: There are various options for rejoining molecules when the pKAN and pAMP fragments are combined.…

Q: plasmid has a total size of 2686 bp. There is an EcoRI recognition site at position 439, BamHI…

A: Plasmid  It is an extra chromosomal piece of DNA in the cell.

Q: You are studying a new plasmid, and you digest the plasmid with three restriction enzymes: Eco RI…

A: The use of restriction enzyme is an essential tool in mapping the circular and linear DNA plasmids.

Q: If the F plasmid does not have the gene encoding Fino, this F plasmid would Select one: a. become…

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Q: The map of plasmid pUC19 is shown below.  The restriction site coordinate is the position of the…

A: Introduction Plasmid Vectors: these are like the artificial vehicle to deliver our gene of insert…

Q: You have another circular plasmid. Complete and effective digestion of this plasmid with a…

A: The restriction enzymes are endonuclease that means they can cut the DNA from the middle. They are…

Q: You are studying a new plasmid, and you digest the plasmid with three restriction enzymes: Eco RI…

A: The plasmid map can be created by checking the individual band patterns and band patterns with a mix…

Q: You are studying a new plasmid, and you digest the plasmid with three restriction enzymes: Eco RI…

A: Introduction: Gel electrophoresis: The technique of separating DNA fragments as per their size is…

Q: The activity of restriction enzymes may produce fragments with sticky ends.  Sticky ends are   a) a…

A: Correct option is (c). Restriction enzyme, also called as restrictions endonucleases,  is a…

Q: An E. coli genomic DNA fragment is run out on a gel undigested (U), and then with two different…

A: Restriction enzymes are those enzyme which recognise a specific recognition site in DNA and cuts the…

Q: A newly discovered insect has a genomic DNA that does not contain any repetitive DNA or any intronic…

A: Biotechnology is the use of our understanding of biological processes to develop useful applications…

Q: The partial sequence of one strand of a double-stranded DNA molecule is5′ – – –…

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Q: You have two cell cultures, each containing a different plasmid. The first plasmid is -5kbp long and…

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Q: U have the plasmid pUC18/19, which is a circular plasmid that consists of 2686 bp. What would the…

A: A restriction endonuclease or restriction enzyme is a bacterial enzyme that cuts dsDNA into…

Q: An 9 kb circular plasmid is cut with the EcoRI restriction enzyme, and the reaction products are run…

A: Restriction enzymes are special enzymes that are known as nucleotide scissors as their role in cells…

Q: Redraw Figure 10-6 with the goal of adding one EcoRIend and one XhoI end. Below is the Xhol…

A: There are specific regions in the DNA, which have specific sequences called restriction sites,…

Q: The recognition site of some restriction enzymes are shown. Which will produce sticky ends?     A.…

A: Restriction enzymes also known as restriction endonucleases are the enzymes which acts on the…

Q: Which of the following sequences, in the double-stranded form, can probably be recognized by a…

A: Restriction enzymes are those enzymes that specifically bind to specific DNA sequences and these…

Q: Eco R1 Multiple Clone Site (MCS) AmpR Hind 3 LacZ" Plasmid Lacl Amp = Ampicillin Resistance gene Ori…

A: The small DNA piece that is maintained stably in an organism is called a cloning vector. The foreign…

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A: The EcoRI restriction site is present in tetracycline resistance gene. This meand that while…

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A:

Q: RFLP's result from mutations in: 1) genes which specify restriction enzymes 2) restriction enzyme…

A: Introduction :- DNA markers are also called Molecular markers . They represent the position specific…

Q: The recognition sites for the restriction enzymes BamHI, Xbal, and Bglll are G^GATCC, T^CTAGA, and…

A: Since it is the multiple choice question i will provide answer in next step.

Q: FIGURE 2 shows the only suitable DNA restriction site in a plasmid DNA vector that can be cleaved.…

A: Restriction enzymes are the most important tool in genetic engineering. These enzymes have the…

Q: What is a vector? Which vector did you choose? Explain why you made that choice. What enzyme(s) will…

A: Since you have asked a question with multiple subparts, we can only answer the first three subparts…

Bacterial Genomics

The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.

Transformation Experiment in Bacteria

In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.

Plasmids and Vectors

The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.