The absorbance for a dye sample of unknown concentration is measured and the corrected absorbance is 0.666. The standard curve created is found to have this linear equation: y = 0.153x + 0.043 Calculate the concentration (ug / mL) of the dye sample. 4.54 3.33 4.07 5.17 2.07
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The absorbance for a dye sample of unknown concentration is measured and the corrected absorbance is 0.666. The standard curve created is found to have this linear equation: y = 0.153x + 0.043 Calculate the concentration (ug / mL) of the dye sample.
4.54
3.33
4.07
5.17
2.07
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Solved in 2 steps
- The absorbance of an unknown dye solution is measured to be 1.06 by a spectrometer. The calibration plot of the spectrometer is provided below. What is the molar concentration of the unknown sample (in mole L)? Absorbance vs. Molarity 18 y3 67024x-0.0343 16 14 12 1 0.8 0.6 0.4 0.2 01 0.2 0.000005 00001 0000015 Concentration of solutions, mol/L 0.00002 0.000025 000003 0 163e-5 O 2.35e-6 0 163 0 235 AbsorbanceThe spectroscopic data in the table is generated with five solutions of known concentration. Concentration (M) 0.0133 m= 0.0266 0.0532 0.106 0.213 Absorbance 0.1271 What is the intercept of the linear regression line? 0.08531 0.5388 1.069 Use a spreadsheet program, such as Microsoft Excel, to graph the data points and determine the equation of the best-fit line. 1.954 What is the slope of the linear regression line formed by these points? M-1A solution of potassium permanganate of unknown concentration was analyzed against a standard of various concentrations. Table 1. Data for Standard Calibration Curve Absorbance at 525 nm Potassium permanganate Standard (mol/Liter) 0.00040 0.104 0.00080 0.258 0.00120 0.453 0.00160 0.682 0.00200 0.894 What is the concentration of potassium permanganate if its absorbance is 0.465? none of the above 0.00137 M KMnO4 O 0.00155 M KMnO4 0.00117 M KMnO4 0.00153M KMnO4
- 6. Blue Blue dye stock solution 0.293 M Absorbance at 630 nm 0.00265 Calibration curve y = 0.0833x A solution is prepared by diluting 2.79 mL of the blue dye stock solution to 25.00 mL. The measured absorbance for the prepared solution is listed in the data table. (a) What is the theoretical molar concentration? [Blue]theoretical x 10 |M (b) What is the experimental molar concentration? [Blue]experimental x 10 M (c) What is the percent error? Percent error (blue) = %To test a spectrophotometer’s accuracy, a solution of 60.06 ppm K2Cr2O7 is prepared and analyzed. This solution has an expected absorbance of 0.641 at 350.0 nm. Several aliquots of the solution produce the following absorbance values. 0.640 0.638 0.640 0.639 0.640 0.639 0.638 is there any significant difference between the experimental mean and the expected value at a 99.9% confidence level? Question 21 options: Yes because tcal<tcritical Yes because tcal>tcritical No because tcal<tcritical No because tcal>tcriticalAfter determining the absorbance of several standards of known concentration, the trendline for a calibration curve (AKA a standard curve) plotting absorbance (y-axis) against concentration (M, x-axis) is determined to be y = 4.943x + -0.0001 The absorbance of a solution of unknown concentration is determined to be 0.48. Calculate the concentration of the unknown solution in M. Give your answer to three decimal places.
- After determining the absorbance of several standards of known concentration, the trend line for a calibration curve plotting absorbance (y-axis) against concentration (uM, x-axis) is determined to be y=4.244x+0.0000. The absorbance of a solution of unknown concentration is determined to be 0.604. Calculate the concentration of the unknown solution in microM. Give answer to 3 decimal placesThis graph shows an absorbance vs concentration curve for Co2*(aq) solution. Sample Standard Curve 1.20 1.00 800 .600 a 400 e 200 20 02 0 080 100 Co2. conc. (M) .020 040 OGO 120 140 160 • Slope - 6.414 Intercept -7.799991E 03 Con. Coef - 9791 Assuming that Beer's Law holds, A = Elc, and the cuvette was a standard 1-cm optical path, what is the molar absorptivity coefficient for the Co2* solution? a. 3.58 x 103 cm" o b.3.58 x 103 cm M-1 OC 7.80 x 103 cm1M-1 O d. 6,414 cm1M1Blue Blue dye stock solution 0.293 M Absorbance at 630 nm 0.00265 Calibration curve y = 0.0833x A solution is prepared by diluting 2.81 mL of the blue dye stock solution to 25.00 mL. The measure absorbance for the prepared solution is listed in the data table. (a) What is the theoretical molar concentration? [Blue]theoretical | × 10 |M (b) What is the experimental molar concentration? [Bluelexperimental x 10 M (c) What is the percent error? Percent error (blue) =
- A liquid sample was analyzed for Fe³+. A plot of absorbance (y-axis) vs. concentration (ppm; x-axis) of the external standards produced a calibration line with an equation y = 0.2256x -0.0903 (R² = 0.9973). The absorbance of the sample was 1.25. Determine the amount of Fe³+ in the sample (in ppm).A solution of potassium permanganate of unknown concentration was analyzed against a standard of various concentrations. What is the concentration of KMNO4 if its absorbance is 0.678? Table 1. Data for the Standard Calibration Curve KMNO4 Standard Solution (mol/L) Absorbance at 525 nm 0.000400 0.112 0.000800 0.295 0.000120 l0.443 0.000160 0.701 0.00200 0.932 O 0.00173 M 0.00194 M O 0,00189 O 0.00155 M10. For the following absorbance data, determine the concentration range that corresponds to the linear range of the standard curve. [MM] 0.001 0.005 0.01 0.05 0.1 0.5 1.0 1.5 Absorbance 0.05 0.06 0.15 0.30 0.70 0.90 0.92 0.97