Suppose you want to determine whether a particulargene X is important for specification of the pharynx,but mutations in this same gene disrupt embryonicdevelopment well before pharyngeal structures appear.How could you use myo-2::GFP, the myo-2 promoter,the DNA sequence of gene X, and your knowledge ofRNA interference (RNAi) to generate worms that lackgene X expression in the pharynx but express gene Xin all other tissues in which it is expressed in wildtype C. elegans?

Suppose you want to determine whether a particulargene X is important for specification of the pharynx,but mutations in this same gene disrupt embryonicdevelopment well before pharyngeal structures appear.How could you use myo-2::GFP, the myo-2 promoter,the DNA sequence of gene X, and your knowledge ofRNA interference (RNAi) to generate worms that lackgene X expression in the pharynx but express gene Xin all other tissues in which it is expressed in wildtype C. elegans?

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Description: Suppose you want to determine whether a particulargene X is important for specification of the pharynx,but mutations in this same gene disrupt embryonicdevelopment well before pharyngeal structures appear.How could you use myo-2::GFP, the myo-2 promo

Biology Today and Tomorrow without Physiology (MindTap Course List)

5th Edition

ISBN:9781305117396

Author:Cecie Starr, Christine Evers, Lisa Starr

Publisher:Cecie Starr, Christine Evers, Lisa Starr

Chapter10: Biotechnology

Section: Chapter Questions

Problem 4DID

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The transcription factor Pax6 is required continuallyduring the life of a mouse (or a human) for the development and maintenance of the retina. HomozygousPax6 knockout mice die soon after birth because Pax6protein is also required in essential organs, such as thepancreas.a. In order to study the role of Pax6 in eye development, a researcher wants to generate a mouse thatexpresses Pax6 everywhere except in its eyes.Describe how you could construct such a mouse.b. Suppose the scientist wants to create a mousesimilar to that in part (a), but in which the eye cellsfrom which Pax6 function has been removed nowexpress GFP. Marking the cells in this way willallow the investigator to see the shapes of the Pax6−eye cells more easily than if they did not expressGFP. Diagram a Pax6 gene construct that wouldenable her to do this experiment.
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