Suppose you observed a bacterium growing on Mannitol Salt agar (MSA) with a yellow colony appearance in the clinical microbiology lab. Name the genus and species you are most likely observing; state its most likely oxygen needs and salt requirements; and predict if this organism would grow on MacConkey agar or Colistin Nalidixic Acid agar. If you want to control the growth of this organism in a healthcare setting, predict what disinfectants would be best to use and their modes of action. Explain your reasoning

Suppose you observed a bacterium growing on Mannitol Salt agar (MSA) with a yellow colony appearance in the clinical microbiology lab. Name the genus and species you are most likely observing; state its most likely oxygen needs and salt requirements; and predict if this organism would grow on MacConkey agar or Colistin Nalidixic Acid agar. If you want to control the growth of this organism in a healthcare setting, predict what disinfectants would be best to use and their modes of action. Explain your reasoning

Comprehensive Medical Assisting: Administrative and Clinical Competencies (MindTap Course List)

6th Edition

ISBN:9781305964792

Author:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Publisher:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Chapter42: Basic Microbiology

Section: Chapter Questions

Problem 4CR

See similar textbooks

Similar questions

MacConkey agar inhibits the growth of Gram-positive bacteria while encouraging the growth of Gram-negative bacteria. It also can determine if some bacteria use lactose. Define what type(s) of media MacConkey agar is, using as many terms in the answer list as are applicable.
If lysozyme is added to a microbial culture. Predict what will be observed if the culture is affected by the action of lysozyme. A change in color will be visible in the tube. An increase in absorbance will be observed over time. A decrease in absorbance will be observed over time. An increase in turbidity will be observed over time.
what information about the identity and physiology of a bacterium or yeast can be learned from examing colonies of unknown microorganisms growing on solid media (agar)
For the following agar media used, list their significant components and explain how they react. Provide one example of a bacterium that will show change to the components (positive), and one example of a bacterium that will not show change or grow on the medium (negative).
An unknown bacterium produces colorless colonies when inoculated onto an EMB plate. Predict what you would see if you inoculated that same unknown onto the Mannitol salt agar media?
Is the mannitol salt agar (MSA) a complex or defined medium? Explain based on Composition. What kind of media based on what kind of microorganisms it allows to grow
This diagram shows the filter paper method used to evaluate the inhibitory effect of chemical agents, heavy metals, and antibiotics on bacterial growth. A culture of a test bacterium is spread uniformly over the surface of an agar plate. Small filter paper discs containing the material to be tested are then placed on the surface of the medium. A disc that has been soaked in sterile distilled water is sometimes added as a control. After incubation, a lawn (film of growth) will cover the plate, but a clear zone will surround those discs that contain an inhibitory compound. The size of the zone reflects several factors, one of which is the effectiveness of the inhibitory agent. What are two other factors that might affect the size of the zone of inhibition? What is the purpose of the control disc? If a clear area were apparent around the control disc, how would you interpret the observation?
You inoculate a species of bacteria on a MacConkey agar. It normally shows positive for growth and ability to breakdown lactose, but does not. After talking to your lab technician about this you learn the MacConkey agar was not made the correct way. Please discuss what method(s) can be performed and the results that would help confirm what the MacConkey agar would normally show.
After quick-characterization by staining, specialized growth media can be used to indicate further biochemical characteristics of an unknown organism. These tests use visual outcomes, such as colors, patterns, and changes in appearance. The results can be interpreted to then assign a characteristic to the unknown organism. For example, a color change may happen when an unknown organism is incubated in a mixed sugar broth. The color could indicate which sugar was (or was not) used by the bacterium for energy. a) explain in your own words how to perform specialized media tests to find an unknown bacteria
Given the scenario, compute for the total volume of the culture media solution (milliliter or liter) and dehydrated media (grams). Scenario: The students of a Microbiology class were tasked to transfer or subculture a pure culture of Escherichia coli bacterium in five 7 mL nutrient broth and five petri dishes of nutrient agar with 20 mL capacity each. Based on the instruction bottles for nutrient broth and nutrient agar, preparation of the culture media is as follows. Nutrient broth: 8 g/liter Nutrient agar: 28 g/liter Formula: C1V1 = C2V2 *Concentration *Volume Computation: What are the answers to the following. Weight in grams of nutrient broth: _________ Distilled water in mL for nutrient broth: __________ Weight in grams of nutrient agar __________ Distilled water in mL for nutrient agar: ____________
Answer the question briefly and concisely. The Mannitol salt agar (MSA) only allows the growth of halophiles (salt-loving microbes);nonhalophiles will not grow. Among the halophiles, mannitol fermenters will produce acid that turnsthe pH indicator yellow; mannitol nonfermenters leave the medium red. Onto MSA you inoculate ahalophilic mannitol fermenter and a halophilic mannitol nonfermenter. In this case, the medium isacting as (a) __________ medium(s).A. SelectiveB. DifferentialC. Selective and differentialD. Enriched
For each of the following situations, describe how bacteria on these growth. media would appear on the plate: A. On MacConkey agar, a bacterium that grows on the medium but is negative for lactose fermentation B. On EMB agar, a bacterium that grows on the medium but is positive for lactose fermentation C. On mannitol salt agar, a bacterium that failed to grow on the medium D. On mannitol salt agar, a bacterium that grew on the medium and fermented mannitol