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Substrate Concentration (mM) Velocity (mM/sec) 2.5 2 4 6.3 7.6 10 20 1. Using this information construct a Michaelis-Menten curve. 2. Using this information construct a Lineweaver-Burk plot. 3. Using the Lineweaver-Burk plot determine the values of Km and Vmax-

Substrate Concentration (mM) Velocity (mM/sec) 2.5 2 4 6.3 7.6 10 20 1. Using this information construct a Michaelis-Menten curve. 2. Using this information construct a Lineweaver-Burk plot. 3. Using the Lineweaver-Burk plot determine the values of Km and Vmax-

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Hello can someone please help me graph 1&2 and help find the vmax and km

Lisa is running an experiment to determine the kinetic parameters (Km and Vmax) of the enzyme that she is studying. She has run a series of experiments where she changed the amount of substrate added and measured the accumulation of product over a 5 second time interval. She used this data to calculate the dependence of the rate of the reaction on substrate concentration. Her results listed below. Substrate Concentration (mM) Velocity (mM/sec) 2.5 4 6.3 5 10 20 7.6 6. 1. Using this information construct a Michaelis-Menten curve. 2. Using this information construct a Lineweaver-Burk plot. 3. Using the Lineweaver-Burk plot determine the values of Km and Vmax.
Transcribed Image Text:Lisa is running an experiment to determine the kinetic parameters (Km and Vmax) of the enzyme that she is studying. She has run a series of experiments where she changed the amount of substrate added and measured the accumulation of product over a 5 second time interval. She used this data to calculate the dependence of the rate of the reaction on substrate concentration. Her results listed below. Substrate Concentration (mM) Velocity (mM/sec) 2.5 4 6.3 5 10 20 7.6 6. 1. Using this information construct a Michaelis-Menten curve. 2. Using this information construct a Lineweaver-Burk plot. 3. Using the Lineweaver-Burk plot determine the values of Km and Vmax.
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