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state two factors that determine which ions predominate in a primary absorbed layer
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- Explain why is FAAS a good method of quantifying Ca in milk?A direct ELISA experiment was done to determine the amount of protein present in a sample. A standard curve was prepared by plotting log Absorbance versus log concentration of protein in (ng/mL). The equation of the line is Y= 0.895 X -1.25. If an unknown protein shows an absorbance of 1.23. Calculate the concentration of this protein in the sample in (ng/mL).Tabulate five advantages and limitations of microwave acid digestion over hot plate acid digestion sample preparation methods prior to spectroscopic metal detection.
- A medical lab technician creates a standard solutions of percent hemoglobin in whole blood. The technician measures the absorbance of each solution at 520 nm and creates a standard curve of the absorbance versus percent hemoglobin in whole blood and finds the linear equation below, with an R2 of 0.9985. y= 0.0523x + 0.072 A patient sample has an absorbance of 0.893. Using the standard curve data, calculate the percent hemoglobin in the patient sample.A group of students made 5 standard solutions and measured their corresponding absorbance values at a wavelength of 525.0 nm to generate the graph below. Absorbance of Standard Solutions to Find Concentration of Unknown X Solution 0.50 y = 75.9x + 0.0045 R? = 0.9946 0.40 %3D 0.30 0.20 0.10 0.00 0.001 0.002 0.003 0.004 0.005 0.006 Concentration of X (mole/L) The colorless reactants V and W form unknown X from the reaction: 2V + W 5 X A solution is prepared by mixing together the following: Volume 0.0150 M V (mL) Volume 0.0150 M W (mL) 6.00 4.00 This solution reaches equilibrium, and then is placed in a 1.00 cm wide cuvet and inserted into the spectrometer, producing an absorbance reading of 0.275 at a wavelength of 525.0 nm. Calculate Keg for the above reaction, including units. AbsorbanceA medical lab technician creates a standard solutions of percent hemoglobin in whole blood. The technician measures the absorbance of each solution at 520 nm and creates a standard curve of the absorbance versus percent hemoglobin in whole blood and finds the linear equation below, with an R2 of 0.9985. y=0.0523x+0.072 A patient sample has an absorbance of 0.915. Using the standard data curve data, calculate the percent hemoglobin in the sample
- Calculate the specific absorbance ratio (SAR), where A620 = 0.166 and A280 = 0.557When using cuvettes to measure the absorbance of a solution, why is it necessary to only hold the cuvettes near the top?The intensity of red light (680 nm) and sorted by a solution of green dye was found to be 1.99 V and the intensity of light when no dye was present (the blank) was found to be 2.69 V.What is the absorbable of the solution of green dye in three significant figures
- The same water sample from the cave was subjected to an analysis to determine its nitrite concentration. Using a spectrophotometer, the following results were obtained for standard solutions of nitrite and for the unknown. Solution Absorbance at 550 nm 2.00 µM 0.065 6.00 µM 0.205 10.00 μΜ 0.338 14.00 μΜ 0.474 18.00 μΜ 0.598 Unknown 0.402 a. Draw a calibration curve base on the data from the standards. b. Find the slope and intercept of the curve. c. Determine the concentration of nitrite in the water sample.The best fit curve line to an absorbance vs. concentration plot for standard solutions of a dye has a slope of 0.1815 mL/mg and intercept of 0.0477. A sample solution prepared of the same dye has an absorbance of 0.464 at the wavelength used to establish the Beer's law plot. What is the concentration of dye in the prepared solution? Select one: O a. 0.132 mg/mL b. 2.29 mg/mL O c. 0.464 mg/mL O d. 2.82 mg/mL e. 0.0365 mg/mL Next page Law Tutorial Part 2 Jump to... Exp #9 - Laboratory Practical► Worksheet étv AChosen wavelength: 400 EDTA Volume (mL) Absorbance O mL 0.445 1 ml 0.439 2 ml 0.434 3 ml 0.428 4 ml 0.388 5 ml 0.352 6 ml 0.351 7 ml 0.320 8 ml 0.289 9 ml 0.275 10 mL 0.263 11 mL 0.256 Explain why the selected wavelength was chosen for inspection of the titration.