Select the correct statements about CRISPR-Cas9 CRISPR-Cas9 can only introduce mutations in the target DNA by introducing a mutated sequence it includes a nuclease which cuts DNA at a specified location it is a naturally occurring system present in eukaryotes for defense against bacteria it contains a guide RNA which can be altered to find the desired target within the genome

Select the correct statements about CRISPR-Cas9 CRISPR-Cas9 can only introduce mutations in the target DNA by introducing a mutated sequence it includes a nuclease which cuts DNA at a specified location it is a naturally occurring system present in eukaryotes for defense against bacteria it contains a guide RNA which can be altered to find the desired target within the genome

Name: 58. (more than one correct answer) Select the correct statements about
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Description: 58. (more than one correct answer) Select the correct statements about CRISPR-Cas9CRISPR-Cas9 can only introduce mutations in the target DNA by introducing a mutated sequenceit includes a nuclease which cuts DNA at a specified locationit is a natural

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter16: Reproductive Technology, Genetic Testing, And Gene Therapy

Section: Chapter Questions

Problem 13QP: The prospect of using gene therapy to alleviate genetic conditions is still a vision of the future....

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The prospect of using gene therapy to alleviate genetic conditions is still a vision of the future. Gene therapy for adenosine deaminase deficiency has proved to be quite promising, but many obstacles remain to be overcome. Currently, the correction of human genetic defects is done using retroviruses as vectors. For this purpose, viral genes are removed from the retroviral genome, creating a vector capable of transferring human structural genes into sites on human chromosomes within target-tissue cells. Do you see any potential problems with inserting pieces of a retroviral genome into humans? If so, are there ways to combat or prevent these problems?
Which of the following functions can be fulfilled by Cas proteins? Form sticky ends to insert genes Create mutations in target DNA Cut RNA Cut sequences at an alternative site Guide CRISPR to the target DNA Cut target DNA
Matching type Choices are in the picture 1. simultaneous and rapid process producing mRNA and polypeptide 2. cleaving the polypeptide by adding water 3. three initiation factors are required to commence the process 4. removal of gene segment disrupting the message 5. single mRNA codes for the proteome
Transcribe the given DNA sequences to mRNA. DNA: 5'-GATCCGC-3' DNA: 5'-TTACGCTAA-3' DNA: 5'-ACGTCAATGGA-3' DNA: 5'-GCGCGGATTAGCGAT-3' mRNA: 3'- mRNA: 3'- mRNA: 3'- mRNA: 3'- -5' -5' -5' -5'
Match the regulatory RNA to each of the following descriptions. Regulatory RNA that prevents transcription by recruiting DNMT enzymes Regulatory RNA that prevents translation by acting either in cis- or trans- but not by binding RISC Regulatory RNA found only in eukaryotes that is not perfectly complementary to the target RNA [Choose ] [Choose ] [Choose ] antisense RNA siRNA IncRNA miRNA riboswitch <
What is the guide RNA for? It is an RNA sequence that binds to a DNA 5'-NGG sequence where N can be any nucleotide (A, T, C, or G). It is the RNA code upon which the DNA to be inserted is built It connects the DNA sequence to the Cas9 protein It binds to the target DNA sequence, defining where Cas9 should cut the DNA It is inserted into the target DNA sequence, causing the desired gene edit
1. You are investigating a protein that has the amino acid sequence N ... Ala – Thr - Asn – Trp – Lys - Arg - Gly – Phe – Thr ... C within its primary structure. You found that several of the mutations affecting this protein produced shortened protein molecules that terminated within this region. In one of the mutants, the Asn became the terminal (last) amino acid. (a) What DNA single-base changes(s) would cause the protein to terminate at the Asn residue? (b) What other potential sites do you see in the DNA sequence encoding this protein where mutation of a single base pair would cause premature termination of translation? >
Matching type Choices are in the picture 6. RF1 and RF2 recognize the three bases to terminate the process 7. CAAT box is found 80 nucleotides from the actual site 8. rho factor and sequence of uracil in a loop conclude the process 9. sigma factor binds to RNA polymerase in the promoter region 10. methionine is removed
During the [X] phase of CRISPR-Cas9 defense, sequences from a new bacteriophage that infects the cell are chopped by a Cas1/Cas2 complex and integrated into the CRISPR locus, >A Moving to another question will save this response.
The sigma subunit of bacterial RNA polymerase ________________________. is required for the initiation of transcription binds to the RNA polymerase core enzyme must be released from the RNA polymerase for transcription to enter the elongation phase binds to the bacterial promoter sequence All of the other answers are correct.
Which of the following computational tools would you use to determine the amino acid sequence encoded by your expression plasmid insert sequence? Protein Blast ExPASy Translate ExPASy pI/Mw T-COFFEE
Cas9 is a protein that cuts nucleic acids a delivery vector a small DNA binding protein clusters of short palindromic DNA