Review Topic [References) The isoelectric point, pl, of the protein lipase A is 4.9 , while that of nitrate reductase is 4.2. What is the net charge of lipase A at pH 5.1 ? What is the net charge of nitrate reductase at pH 2.5 The isoelectric point of glycine is 5.97 ; arginine, 10.76. During paper electrophoresis at pH 7.1 , toward which electrode does glycine migrate? During paper electrophoresis at pH 7.5 , toward which electrode does arginine migrate?

Introduction to General, Organic and Biochemistry
11th Edition
ISBN:9781285869759
Author:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres
Publisher:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres
Chapter31: Immunochemistry
Section: Chapter Questions
Problem 31.72P
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[Review Topics]
(References)
The isoelectric point, pl, of the protein lipase A is 4.9 , while that of nitrate reductase is 4.2 .
What is the net charge of lipase A at pH 5.1 ?
What is the net charge of nitrate reductase at pH 2.5 ?
The isoelectric point of glycine is 5.97 ; arginine, 10.76.
During paper electrophoresis at pH 7.1 , toward which electrode does glycine migrate?
During paper electrophoresis at pH 7.5 , toward which electrode does arginine migrate?
Transcribed Image Text:[Review Topics] (References) The isoelectric point, pl, of the protein lipase A is 4.9 , while that of nitrate reductase is 4.2 . What is the net charge of lipase A at pH 5.1 ? What is the net charge of nitrate reductase at pH 2.5 ? The isoelectric point of glycine is 5.97 ; arginine, 10.76. During paper electrophoresis at pH 7.1 , toward which electrode does glycine migrate? During paper electrophoresis at pH 7.5 , toward which electrode does arginine migrate?
Revlew Topics]
References)
The isoelectric point, pl, of the protein 3a hydroxysteroid dehydrogenase is 6.1, while that of chymotrypsinogen A is 9.1
What is the net charge of 3a hydroxysteroid dehydrogenase at pH 5.1
What is the net charge of chymotrypsinogen A at pH 8.5 7
The isoelectric point of histidine is 7.59 ; threonine, 5.6.
During paper electrophoresis at pH 7.1, toward which electrode does histidine migrate?
During paper electrophoresis at pH 4.5 , toward which electrode does threonine migrate?
Transcribed Image Text:Revlew Topics] References) The isoelectric point, pl, of the protein 3a hydroxysteroid dehydrogenase is 6.1, while that of chymotrypsinogen A is 9.1 What is the net charge of 3a hydroxysteroid dehydrogenase at pH 5.1 What is the net charge of chymotrypsinogen A at pH 8.5 7 The isoelectric point of histidine is 7.59 ; threonine, 5.6. During paper electrophoresis at pH 7.1, toward which electrode does histidine migrate? During paper electrophoresis at pH 4.5 , toward which electrode does threonine migrate?
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