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- 12) If gloves are not worn when handling the cuvette in a spectrophotometry xperiment, how would this affect the absorbance reading (higher, or lower, or same) and ow would it make the concentration look, higher or lower?A mass spectrum with M+ at m/z = 101 with a minor M+1 peak contains what diagnostic atom? I believe the substance talked about may be C6H15N but I am not sure. Please explain.The maximum number of spectra that can be recorded per second by ToF-MS is limited by the time taken by the slowest ions to go from the source to the detector. If the slowest ion took 45.6 microsecond to travel, at what frequency could you record spectra if a new extraction cycle were begun each time this heaviest ion reached the detector? 30000 spectra/s 10000 spectra/s 11000 spectra/s 500 spectra/s 22000 spectra/s
- A researcher is studying cytochrome oxidase, a protein that uses iron to bind diatomic oxygen. She collects two IR spectra, shown below. In the first spectrum, no isotopic labeling is used (top). In the second, 10 has been replaced with 180 (bottom). 820 655 790 0.5- 714 695 733 820 655 755 0.5- 714 695 733 0+ 600 650 700 750 800 850 Vo (cm") Based on the above ratio, which is the most likely source of this vibration, (a) the Fe-O stretch in Fel-O=0, or (b) the O-O stretch in Fel"-O=O? You can assume that the atomic weight of Fe is 56. ww n wwm % TransmissionQ3/Which of the following IR-spectrum has the most suitable for isopropanol, explain? Transmittance % (a) Transmittance % (b) 4000 3000 2500 2009 100 Wave number (cm) при 2008 11:00 Wave number (cm-¹) 1000 1006 M 5406. Relative Intensity Determine the molecular formula that goes with the following mass spectrum. Write this on the spectrum, and draw a structure that fits with this molecular formula. 100 80 69 20 m/z 51.0 52.0 53.0 9.7 6.6 3.3 77.0 18.8 78.0 7.3 79.0 8.9 89.0 7.5 104.0 7.4 105.0 3.2 106.0 72.5 107.0 5.9 140.0 32.0 141.0 100.0 142.0 8.0 143.0 31.7 144.0 2.3 tarantorpille 25 50 75 m/z 100 abou 125 [M]+ 141 amu
- 3. Fill in the following for the correspondence between % transmittance and absorbance: 100.0 % T = 0.0 absorbance 1.00 absorbance = (0.0 % T 25.0 % T = absorbance 0.25 absorbance = % TQUESTION 7 Determine the functional group present in a molecule with the formula C4H9NO and the IR spectrum seen below: LOD TRANSMITTANCE1%1 O 50 D 4000 3291 3086 21 21 M 12 1667 1440 31 21 1373 Alcohol Amide Amine Ketone B 21 15 2977 2936 32 1366 30 2877 42 1297 16 3000 2594 86 1153 32 1655 4 1118 64 1076 70 1041 64 1002 82 936 64 891 81 720 52 603 34 T 2000 466 70 4 HAVENUMBERI-11 1500 1000 500A researcher is studying cytochrome oxidase, a protein that uses iron to bind diatomic oxygen. She collects two IR spectra, shown below. In the first spectrum, no isotopic labeling is used (top). In the second, 160 has been replaced with 180 (bottom). 820 655 790 0.5- 714 695 733 820 655 755 0.5- 714 695 733 600 650 700 750 800 850 V, (cm") Calculate the change in the ratio of reduced masses for this isotope shift. % Transmission
- Mass spectroscopic. What is the isotopic peak pattern of each : Organic compound with 2 bromine atoms. Organic compound with 2 chlorine atoms.Which is the parent ion (M*) in this mass spectrum? 100- 80- Relative Intensity 60 T T T 40- 20- 0 mm 10 20 30 40 43 57 50 60 72 70 m/z 80 90 100 110 120 start NA researcher is studying cytochrome oxidase, a protein that uses iron to bind diatomic oxygen. She collects two IR spectra, shown below. In the first spectrum, no isotopic labeling is used (top). In the second, 160 has been replaced with 180 (bottom). 820 655 790 0.5- 714 695 733 820 655 755 0.5- 714 695 733 0- 600 650 700 750 800 850 Vo (cm") Based on the above ratio, which is the most likely source of this vibration, (a) the Fe-O stretch in Fell-O=0, or (b) the O-O stretch in Fell-O=O? You can assume that the atomic weight of Fe is 56. % Transmission