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Genetics Q9
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- Question 45 Once the RNA primers are replaced the fragments in the lagging strands are sealed by DNA pol I. A True B) FalseQuestion 44 Plasmids to become effective vectors and cloning really successful, they must be double stranded and generate blunt ends after restriction enzyme digestion. A True B) FalseQuèstion 34 Enzyme that joins the DNA fragments O Recombinant DNA Technology O Restriction enzymes O Ligase O Palindromic sequences
- QUESTION NO. 1 Antisense nucleic acids A. complementary to mRNA would enhance translation . B. could result if a gene is inserted downstream of a promoter but in opposite direction to normal. C. can have no clinical uses. D. react only with DNA. E. are necessary for recombinant DNA technology.QUESTION NO. 2 There is a window in which the effect is primarily on viral replication since AZT is much less effective at competing with dTTP for incorporation by cellular DNA polymerases because of the proofreading ability of DNA polymerases. Proofreading activity co maintain the fidelity of DNA synthesis A. occurs after the synthesis has been completed. B. is a function of 3' co 5' exonuclease activity intrinsic to or associated with DNA polymerases. C. requires the presence of an enzyme separate from the DNA polymerases. D. removes mismatched bases in the interior of the chain. E. does nor occur in prokaryotes. QUESTION NO. 3…QUESTION NO. 1 Antisense nucleic acids A. complementary to mRNA would enhance translation . B. could result if a gene is inserted downstream of a promoter but in opposite direction to normal. C. can have no clinical uses. D. react only with DNA. E. are necessary for recombinant DNA technology. QUESTION NO. 2 There is a window in which the effect is primarily on viral replication since AZT is much less effective at competing with dTTP for incorporation by cellular DNA polymerases because of the proofreading ability of DNA polymerases. Proofreading activity co maintain the fidelity of DNA synthesis A. occurs after the synthesis has been completed. B. is a function of 3' co 5' exonuclease activity intrinsic to or associated with DNA polymerases. C. requires the presence of an enzyme separate from the DNA polymerases. D. removes mismatched bases in the interior of the chain. E. does nor occur in prokaryotes.Question 13 If a recombinant plasmid (below) was obtained inserting DNA into the BamHI site, screening for the recombinant plasmid can be done by which of the following technique? A) Plate on agar plates containing tetracycline and ampicillin. (B) Plate on agar plates containing ampicillin, C) Plate the cells on agar containing ampicillin then surviving colonies are plated on another agar plates with tetracycline. D) Plate on agar plates containing tetracycline. Pal Pord- ampr EcoRI ПРИ Ano pBR322 (4363 bases) Prull BamHI Sall let" Aval -Sall
- QUESTION 19 Match the major enzyme involved in the techniques/steps we discussed in class v PCR A. Reverse Transcriptase v CDNA synthesis B. any enzyme that can cleave a chromogenic substrate v Cutting a plasmid for cloning C. Cas9 v "Gluing" an insert into a plasmid D. DNA polymerase v ELISA E. Restriction enzymes v Knocking out a gene at a specific targeted sequence F. LigaseQuestion 11 Each cycle of amplification in PCR involves all of the steps EXCEPT: A) annealing of oligodeoxyribonucleotide primers to DNA. B) the addition of fresh dNTPs to the reaction mixture. C reaction with DNA polymerase at approximately 70°C. D) thermal denaturation of the target duplex DNA.Question 12 The "natural" function of restriction endonucleases is to A help bacteriophages infect cells. B) regulate gene expression from specific promoters. protect bacterial cells which have them. D) remove chromatin from histones.
- Question 36 DNA molecules can perform their function in replication and transcription as long as the hydrogen bonds between the bases remain intact. A) True B) FalseQuestion 11 1. An 9 kb circular plasmid is cut with the EcoRI restriction enzyme, and the reaction products are run on a DNA gel and stained with ethidium bromide. Bands of 1.0, 3.0 and 5.0 kb are seen. 2. When the same plasmid is cut with both the EcoRI and BamHl restriction enzymes (a double digest), bands of 1.0, 1.5, 3.0, and 3.5 kb are seen. From these results, it can be concluded tha v[ Select ] cuts within the BamHI [ Select ] v kb [Select EcoRIQuestion 8B When scientists first discovered SARS-COV2, they were able to generate a sequence of the viral genome quickly. However, before they were able to do next-gen DNA sequencing on the virus, they had to do a major step before they could get a genome sequence. What did they have to do before carrying out DNA sequencing? Why was this step necessary? (hint: consider what type of virus this is)